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Öğe Combining anti-cancer drugs with artificial sweeteners: Synthesis and anti-cancer activity of saccharinate (sac) and thiosaccharinate (tsac) complexes cis-[Pt(sac)2(NH3)2] and cis-[Pt(tsac)2(NH3)2](Elsevier Inc., 2014) Al-Jibori S.A.; Al-Jibori G.H.; Al-Hayaly L.J.; Wagner C.; Schmidt H.; Timur S.; Barlas F.B.; Subasi E.; Ghosh S.; Hogarth G.The new platinum(II) complexes cis-[Pt(sac)2(NH3)2] (sac = saccharinate) and cis-[Pt(tsac)2(NH3)2] (tsac = thiosaccharinate) have been prepared, the X-ray crystal structure of cis-[Pt(sac)2(NH3)2].H2O reveals that both saccharinate anions are N-bound in a cis-arrangement being inequivalent in both the solid-state and in solution at room temperature. Preliminary anti-cancer activity has been assessed against A549 human alveolar type-II like cell lines with the thiosaccharinate complex showing good activity. © 2014 Elsevier B.V. All rights reserved.Öğe Gold nanoparticle conjugated poly(p-phenylene-?-cyclodextrin)-graft-poly(ethylene glycol) for theranostic applications(John Wiley and Sons Inc., 2019) Barlas F.B.; Aydindogan E.; Arslan M.; Timur S.; Yagci Y.Fluorescent conjugated polymers gained interest in the last decades for both imaging and targeting tumor cells for the purpose of diagnosis and treatment of cancer. In the light of this objective conjugated poly(p-phenylene) possessing ?-cyclodextrin (?-CD) units in the main chain and poly(ethylene glycol) side chains is used as an imaging and therapeutic agent to target U87 and Vero cells. Additionally, imaging quality and therapy efficiency of the bare graft copolymer and its gold nanoparticle (AuNP) conjugated form were investigated and compared. It is observed that ?-CD is effective not only for the imaging of the tumor cells, but also as a radiotherapy agent. Conjugation of the polymer with the AuNPs provides significant improvement in the therapeutic efficiency. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47250. © 2018 Wiley Periodicals, Inc.Öğe Herbal infusions of black seed and wheat germ oil: Their chemical profiles, in vitro bio-investigations and effective formulations as Phyto-Nanoemulsions(Elsevier, 2015) Gumus Z.P.; Guler E.; Demir B.; Barlas F.B.; Yavuz M.; Colpankan D.; Senisik A.M.; Teksoz S.; Unak P.; Coskunol H.; Timur S.The reported studies related to black seed oil (BSO) and wheat germ oil (WGO) have illustrated that they have a wide range of biological activities. Therefore, enhancing the amount of bio-active compounds that caused higher cell based anti-oxidative effect as well as cell proliferation, etc. in seed oils, infusion of crude plant material has been gained importance as a traditional technique. Herein, we accomplished the infusion of Calendula flowers that also contains many phyto-constituents into BSO and WGO. After the infusion of oils, the change of phytochemical amount was investigated and evaluated according to the oils by chromatography, radical scavenging activity. Subsequently, for investigating the biological impact upon live cells, cytotoxicity, cell-based antioxidant capacity, wound healing and radioprotective activity were tested with monkey kidney fibroblast like cells (Vero) and HaCaT keratinocytes. In vitro cell based experiments (wound healing and radioprotective activity) confirmed that Calendula infused BSO and WGO have greater bio-activity when compared to those plain forms. The herbal oils prepared with an effective extraction technique were incorporated into nanoemulsion systems which will be then called as 'Phyto-Nanoemulsion'. After herbal oil biomolecules were encapsulated into nanoemulsion based delivery systems, the designed formulations were investigated in terms of biological activities. In conclusion, these preparations could be a good candidate as a part of dermal cosmetic products or food supplements which have the therapeutic efficiency, especially after radio- or chemotherapy. © 2015 Elsevier B.V..Öğe Multimodal theranostic assemblies: Double encapsulation of protoporphyrine-IX/Gd3+ inniosomes(Royal Society of Chemistry, 2016) Barlas F.B.; Demir B.; Guler E.; Senisik A.M.; Arican H.A.; Unake P.; Timur S.Theranostic therapy is one of the most promising methods in cancer research, which simultaneously allows the treating and real-time monitoring of cancer. In the present study, a new method was developed to achieve advanced theranostic therapy by double encapsulation of gadolinium nanoparticles (GdNP) and protoporphyrin IX (PpIX) into niosomes. Hereby, niosomes are used for encapsulation of GdNP and PpIX, which will be called 'Gd-PpIX-NI'. Niosomes are chosen as the encapsulation material owing to their high biocompatibility, physical and chemical stability, and fair price. On the other hand, GdNP and PpIX are good sensitizers for radiotherapy (RT) and, particularly, porphyrin structures are one of the most studied agents for photodynamic therapy (PDT). In this study, a multimodal treatment was performed with the combination of PDT and RT by using human alveolar type-II (ATII)-like cells (A549) and human cervical cancer cell line (HeLa). Moreover, 'Gd-PpIX-NI' serves as a dual cell imaging probe that provides both fluorescence and magnetic resonance imaging. Characterization of the sizes and zeta potential of the niosomal vesicles was carried out by dynamic light scattering and atomic force microscopy. To determine the cell viability after treatment with Gd-PpIX-NI, followed by PDT and RT application, the MTT method was used. The results showed that Gd-PpIX-NI assembling was homogeneous and consistent in terms of particle size, which is less than 100 nm. This material has potential as a good candidate for both PDT and RT, as well as diagnosis. © The Royal Society of Chemistry 2016.Öğe Niosomes of Nerium oleander extracts: In vitro assessment of bioactive nanovesicular structures(Editions de Sante, 2017) Gunes A.; Guler E.; Un R.N.; Demir B.; Barlas F.B.; Yavuz M.; Coskunol H.; Timur S.Niosomes are known as non-ionic surfactant-based vesicles and have been used quite a lot for medical applications as a novel drug delivery system in recent years. Herein we describe, the preparation and characterization of niosomes containing oleander (Nerium oleander) (ONs) extract as the herbal compound. In this context, niosomal formulations of two different extracts obtained from oleander roots were prepared by a thin film hydration technique using Tween 60 (non-ionic surfactant) with a cholesterol mixture at 1:1 M ratio. These vesicular structures were characterised by various techniques such as atomic force microscopy, zeta potential and dynamic light scattering size measurements. ONs vesicles are less than 100 nm in size with a good physical stability more than 50 days. Initially, total phenolic and flavonoid contents and antioxidant activities of the extracts were investigated. The methanol extract of oleander roots (MOE) showed higher polyphenolic content and exhibits a better antioxidant activity in compared to the hydro-methanol (20% methanol) extract (MOWE). Total phenolic contents in the MOE and MOWE were calculated as 64.51 ± 0.945 µg/mg and 65.05 ± 0.37 µg/mg gallic acid equivalents, respectively. Encapsulation efficiencies of the vesicles were found as 16.2% for MON (contain MOE) and 13.24% for MWON (contain MOWE). The significant linear correlation was confirmed between the antioxidant activity and total phenolic content of extracts as well as ONs. Cell based cytotoxic activities of methanol extract and MON formulations were also assessed via MTT assay using HeLa and A549 cell lines. © 2016 Elsevier B.V.Öğe A novel DAD type and folic acid conjugated fluorescent monomer as a targeting probe for imaging of folate receptor overexpressed cells(John Wiley and Sons Inc., 2014) Ekiz Kanik F.; Ag D.; Seleci M.; Barlas F.B.; Kesik M.; Hizalan G.; Akpinar H.; Timur S.; Toppare L.We describe a modification and post-functionalization technique for a donor-acceptor-donor type monomer; 6-(4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-2H-benzo[d][1,2, 3]triazol-2-yl)hexan-1-amine. Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and Fourier transform infrared analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via fluorescence microscopy. © 2014 American Institute of Chemical Engineers.Öğe One-pot photo-induced sequential CuAAC and thiol-ene click strategy for bioactive macromolecular synthesis(American Chemical Society, 2014) Doran S.; Murtezi E.; Barlas F.B.; Timur S.; Yagci Y.Conceptually new one-pot photoinduced sequential click reactions were implemented to yield novel block copolymers with the ability for cell adhesion. Poly(?-caprolcatone) possessing clickable functional groups at the chain ends, namely ?-alkynyl-alkenyl-poly(?-caprolactone) (A-PCL-MA), was prepared by ring-opening polymerization of ?-caprolactone using propargyl alcohol in the presence of stannous octoate at 110 °C followed by esterification with methacrylic acid. Azide-functional poly(methyl methacrylate) (PMMA-N3) was prepared independently by atom transfer radical polymerization (ATRP) followed by an azidation process using sodium azide. Finally, A-PCL-MA was reacted with PMMA-N3 and N-acetyl-l-cysteine (NAC) in a one-pot process through photoinduced sequential click reactions to furnish desired bioactive block copolymer (PMMA-b-PCL-NAC). A matrix for cell adhesion was then prepared from the yielded block copolymer PMMA-b-PCL-NAC and cell proliferation on the matrix was measured. Cells from the Vero cell line (African green monkey kidney epithelial) were incubated on the matrix, and after 48 h, they showed greater cell proliferation than the commercially available cell culture plates used as comparison. © 2014 American Chemical Society.Öğe Radioiodinated magnetic nanoparticles conjugated with moxifloxacin: Synthesis and in vitro biological affinities(Taylor and Francis Inc., 2015) Guldu O.K.; Unak P.; Medine E.I.; Barlas F.B.; Muftuler F.Z.B.; Timur S.Magnetic nanoparticles (MNPs) were synthesized and coated with tetraethyl orthosilicate and aminosilane to create functional amino groups. Moxifloxacin (MXF) was conjugated to the modified MNPs using glutaraldehyde as crosslinker. Finally, MXF conjugated magnetic nanoparticles were radiolabeled and their biological affinities such as cell incorporation ratio, cytotoxicity, and their potential as cell imaging probe were investigated using A-549 cells. © 2015 Copyright Taylor & Francis Group, LLC.Öğe Synthesis, characterization and biological application of dinuclear Cu(II) complexes of Schiff base ligands of galactochloralose and ?-chloralose(Elsevier S.A., 2018) Salman Y.; Barlas F.B.; Yavuz M.; Kaya K.; Timur S.; Telli F.Ç.Four novel dinuclear Cu(II) complexes (1a–4a) have been prepared from ONO-tridentate chiral Schiff base derivatives of chloralose (1–4). All the new complexes (1a–4a) have been characterized by Fourier transform infrared spectroscopy, UV–visible spectroscopy and elemental analysis. The complex 2a was further characterized by X-Ray crystallography. And also, the magnetic susceptibility measurement of this complex (2a, µeff 2.61 BM/dimer) was measured at room temperature. The activities of ONO-tridentate chiral Schiff base derivatives of chloralose (1–4) and their dinuclear Cu(II) complexes (1a–4a) on viability of human cervical cancer cell line (HeLa) were investigated by MTT assay. The obtained results indicated that the Schiff base ligand 1 and its dinuclear Cu(II) complex (1a) have excellent cytotoxic activity. Also, dinuclear Cu(II) complexes of the galactochloralose and ?-chloralose derivative Schiff base ligands were screened for their in vitro antimicrobial activity against eight bacterial species as well as yeast species Candida albicans ATCC 10231 using disc diffusion method. Complex 4a exhibited promising in vitro antimicrobial activity with inhibition zone diameter from 10 to 22 mm against the tested Gram-positive bacterial strains and yeast C. albicans. © 2018 Elsevier B.V.Öğe Synthesis, characterization and biological application of dinuclear Cu(II) complexes of Schiff base ligands of galactochloralose and ?-chloralose(Elsevier S.A., 2018) Salman Y.; Barlas F.B.; Yavuz M.; Kaya K.; Timur S.; Telli F.Ç.Four novel dinuclear Cu(II) complexes (1a–4a) have been prepared from ONO-tridentate chiral Schiff base derivatives of chloralose (1–4). All the new complexes (1a–4a) have been characterized by Fourier transform infrared spectroscopy, UV–visible spectroscopy and elemental analysis. The complex 2a was further characterized by X-Ray crystallography. And also, the magnetic susceptibility measurement of this complex (2a, ?eff 2.61 BM/dimer) was measured at room temperature. The activities of ONO-tridentate chiral Schiff base derivatives of chloralose (1–4) and their dinuclear Cu(II) complexes (1a–4a) on viability of human cervical cancer cell line (HeLa) were investigated by MTT assay. The obtained results indicated that the Schiff base ligand 1 and its dinuclear Cu(II) complex (1a) have excellent cytotoxic activity. Also, dinuclear Cu(II) complexes of the galactochloralose and ?-chloralose derivative Schiff base ligands were screened for their in vitro antimicrobial activity against eight bacterial species as well as yeast species Candida albicans ATCC 10231 using disc diffusion method. Complex 4a exhibited promising in vitro antimicrobial activity with inhibition zone diameter from 10 to 22 mm against the tested Gram-positive bacterial strains and yeast C. albicans. © 2018 Elsevier B.V.Öğe Theranostic niosomes as a promising tool for combined therapy and diagnosis: "all-in-one" approach(American Chemical Society, 2018) Demir B.; Barlas F.B.; Gumus Z.P.; Unak P.; Timur S.Because of the great achievement and progress made for the generation of novel nanostructures, theranostic nanoplatforms have been the trending topic because of their intensive capability of therapy and diagnosis. Hence, theranostics have also recently been a generic strategy for personalized medicine. Moreover, traditional therapy modalities limit the use of chemotherapeutic agents for every patient, and this requires more effective drug-carrier systems by designing the formulation of drug in a specified way. Herein, we performed a generic theranostic platform in an "all-in-one" concept by the combination of two therapy modalities with an active targeting approach. To achieve this, 10 nm gold nanoparticles (AuNPs) and protoporphyrin IX (PpIX) were encapsulated into folic acid (FA-)tagged niosome vesicles. The resulting AuNP-PpIX-FA niosomes were characterized, and their particle size was93 ± 17 nm with a high surface charge and encapsulation efficiency (around 85%). In the case of bioapplications for AuNP-PpIX-FA niosomes, folate-receptor-positive [FR(+)] human cervical cancer (HeLa) and FR-negative [FR(-)] human alveolar type II (A549)-like cell lines were examined with the relative control groups of theranostic vesicles. By testing the toxicity of vesicles, nontoxic concentrations were successfully introduced to the cell with the combined treatment of radiotherapy and photodynamic therapy. On the other hand, the cellular uptake of niosomes also showed great potential for FR(+) HeLa cells as the theranostic platform with an all-in-one approach. Copyright © 2018 American Chemical Society.
