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Adjuvant effects of Astragalus saponins Macrophyllosaponin B and Astragaloside VII
(Elsevier Ireland Ltd, 2011) Nalbantsoy, Ayse; Nesil, Tuna; Erden, Secil; Calis, Ihsan; Bedir, Erdal
Aim of the study: The present study was undertaken to evaluate the hemolytic activities of two immunomodulator Astragalus saponins [Macrophyllosaponin B (MacB) from Astragalus oleifolius DC. and Astragaloside VII (Ast VII) from Astragalus trojanus Stev.], and their adjuvant potentials on the cellular and humoral immune responses of Swiss albino mice against BSA. Materials and methods: The hemolytic activity of Mac B and Ast VII was determined using 0.5% rabbit red blood cell. For adjuvant activity, Swiss albino mice were immunized subcutaneously with BSA 100 mu g alone or with BSA 100 mu g dissolved in saline containing Ast VII (30, 60, 120 and 240 mu g), Mac B (30, 60, 90 and 120 mu g) or Freund's adjuvant on Days 1 and 15. Sera and splenocytes were collected 2 weeks after the last immunization for concanavalin A (Con A)-, lipopolysaccharide (LPS)- and BSA-stimulated splenocyte proliferation assay and measurement of BSA-specific antibodies in serum. Results: Mac B and Ast VII showed a slight hemolytic effect, with 0.42% and 0.54% values, respectively, at the highest concentration of 500 mu g/ml. Mac B and Ast VII significantly enhanced the Con A-, LPS-, and BSA-induced splenocyte proliferation in the BSA-immunized mice especially at 120 and 240 mu g (P < 0.001), and 60, 90 and 120 mu g (P < 0.05, P < 0.01 or P < 0.001) doses, respectively. BSA-specific IgG, IgG1 and IgG2b antibody titers in serum were also significantly enhanced by Ast VII (120 mu g). Mac B (90 mu g) and Freund's as compared to the control group (P < 0.01 or P < 0.001). Moreover, the IFN-gamma and IL-4 levels in the sera were detected using ELISA two weeks after the last immunization. Ast VII and Mac B were also found to stimulate IFN-gamma production such as Freund's, two weeks after the last immunization at doses of 120 mu g and 90 mu g, respectively, as compared to the control. Conclusion: Results show that Ast VII and Mac B generate important specific antibody and cellular response against BSA in mice, proving their potentials as a new class saponin adjuvant. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
Adjuvant potency of Astragaloside VII embedded cholesterol nanoparticles for H3N2 influenza vaccine
(Tubitak Scientific & Technical Research Council Turkey, 2020) Genc, Rukan; Yakubogullari, Nilgun; Nalbantsoy, Ayse; Coven, Fethiye; Bedir, Erdal
Adjuvants are substances that increase the immune response to a given antigen. in the development of novel vaccine adjuvants/systems, saponins are one of the most attractive molecules due to their altered immunomodulatory activities. in this study, we tried to develop PEG (polyethylene glycol)/cholesterol-based lipid nanoparticles (LNPs) to deliver the Astragaloside VII (AST-VII) and potentiate adjuvant properties of AST-VII for the influenza vaccine. in the formation of PEG/cholesterol/AST-VII-based LNPs (PEG300: Chol-AST-VII LNPs), 3 different primary solvents (acetone, ethanol, and chloroform) were evaluated, employing their effects on hydrodynamic particle size, distribution, surface chemistry, and colloidal stability. Prepared nanoparticles were simply admixtured with inactivated influenza antigen (H3N2) and applied to PMA (phorbol 12-myristate 13-acetate)-ionomycin treated human whole blood to evaluate their cytokine release profile. PEG300: Chol-AST-VII LNPs (80.2 +/- 7.7 nm) were obtained using chloroform as a desolvation agent. Co-treatment of PMA-ionomycin with AST-VII and PEG300: Chol-AST-VII LNPs significantly increased the levels of IL-2 and IFN-gamma, compared to PMA-ionomycin alone. in the presence of H3N2, AST-VII was able to augment IL-17A, while PEG300: Chol-AST-VII LNPs stimulated the production of IFN-gamma. Hemolysis was only observed in PEG300: Chol-AST-VII LNPs (250 mu g/mL) treatment. AST-VII and AST-VII-integrated LNPs could be used as efficacious adjuvants for an inactivated H3N2 vaccine in vitro, and cytokine response through Th1/Th17 route was reported.
Anthemis tricolor Containing Unusual Totarol with Cytotoxic and Acetylcholinesterase-Inhibitory Activity
(Wiley-V C H Verlag Gmbh, 2024) Yucel, Yasemin Yucel; Servi, Hueseyin; Polatoglu, Kaan; Nalbantsoy, Ayse
Anthemis tricolor is an endemic species of Cyprus, and there is very limited information on its chemistry and pharmacological activities. The study aims to identify the in-vitro cytotoxic and acetylcholinesterase activities of Anthemis tricolor. The compounds responsible for the activities were also identified. Potent extracts of A. tricolor were subjected to preparative isolation and spectral structure determination studies. The chloroform extract contained many components, and due to the small quantity of extract available, enough pure compound(s) cannot be obtained for structure determination studies, though the n-hexane extract afforded two known compounds, totarol (1) and taraxasterol (2). The structures of the compounds (1 and 2) were determined by H-1 and( 13)C NMR experiments. The pure compounds were also tested for their acetylcholinesterase inhibitory properties. For compound 1, the IC50 value was found to be 87.88 mu g/mL. However, no inhibition was seen for 2. Anthemis tricolor was established to be a valuable source of pharmacologically active compounds and requires further studies.
Anti-inflammatory phenolic glycosides from endemic Marrubium rotundifolium Boiss.
(Taylor & Francis Ltd, 2024) Akbulut, Tugce Demiroz; Demir, Serdar; Alsakini, Karrar Ali Mohammed Hasan; Nalbantsoy, Ayse; Baykan, Sura
Marrubium rotundifolium Boiss. is an endemic plant distributed in Aegean Region of T & uuml;rkiye. It's traditionally used in Anatolian medicine for treatment of cold and flu, dyspepsia, costiveness and intestinal spasms. However, phytochemical studies on the plant are limited. In the present study, five phenolic glycosides 4',5,6,7-tetramethoxy scutellarein (1), apigenin-7-O-(3-O-E-p-coumaroyl)-beta-d-glucopyranoside (2), tiliroside (3), 4-(beta-d-glucopyranosyloxy) benzoic acid (4), and astragalin (5) were isolated from the aerial parts of M. rotundifolium and their structures were elucidated on the basis of spectroscopic methods (1D and 2D NMR, and MALDI-TOF/MS). Moreover, anti-inflammatory activities of the isolated compounds were evaluated by measurements of interleukins (IL-1 beta, IL-6) and tumour necrosis factor-alpha (TNF-alpha) levels. Tiliroside exhibited the highest potency in all pathways. At concentrations of 3 and 6 mu g/mL, it significantly decreased the levels of pro-inflammatory cytokines IL-1 beta (192.53 and 175.54 pg/mL), IL-6 (925.52 and 946.81 pg/mL), and TNF- alpha (6465.86 and 6267.67 pg/mL). This is the first report on phenolic profile of endemic M. rotundifolium. The anti-inflammatory and antidiabetic activity potential of the plant should be investigated in further studies. {Graphical Abstract}
Anti-inflammatory properties of Centaurea calolepis Boiss. and cnicin against Macrovipera lebetina obtusa (Dwigubsky, 1832) and Montivipera xanthina (Gray, 1849) venoms in rat
(Pergamon-Elsevier Science Ltd, 2018) Demiroz, Tugce; Albayrak, Gokay; Nalbantsoy, Ayse; Gocmen, Bayram; Baykan, Sura
Macrovipera lebetina obtusa (Dwigubsky, 1832) and Montivipera xanthina (Gray, 1849) (Ottoman Viper) are viper snakes from Viperidae family and found in various locations in Anatolia. Both snakes are responsible for major snake bite cases in Turkey Their venoms cause necrosis, hemorrhage, pain and local edema. Centaurea L. (Asteraceae) species draw attention as potential anti-inflammatory sources due to their traditional uses and accomplished studies on this field. C. calolepis Boiss. is an endemic taxon distributed in Aegean and Antalya regions in Turkey. Chloroform extract of C. calolepis and its major compound cnicin, a sesquiterpene lactone, are reported to have strong anti-inflammatory activities in-vitro, by previous studies. In the present study, in-vivo anti-inflammatory activities of C. calolepis chloroform extract and the sesquiterpenoid cnicin against edema induced by Macrovipera lebetina obtusa and Montivipera xanthina venoms were evaluated in the rat model. Protein contents and induction doses of the venoms were determined. Carrageenan and snake venoms were used as inducing agents in paw edema tests. Extract demonstrated strong inhibition on edema at all doses and hours against M. xanthina venom and carrageenan. Inhibition ratio of extract at 25 mg/kg dose (84.13% inhibition) after 0.5 h M. xanthina venom injection was more than indomethacin's value (45.4% inhibition). The extract also showed significant effect also on inflammation caused by M. lebetina obtusa venom at all doses. However, 2.5 mg/kg cnicin was more effective than total extract of C. calolepis against rat paw edema induced by (27.31%) M. lebetina obtusa venom. This is the first study reported therapeutic potential of C. calolepis, an endemic plant of Turkey, in case of snake-bites cause inflammation by venomous species in natural fauna of Anatolia.
Anticancer effect evaluation of nitron complexes
(Elsevier, 2024) Ozdemir, Vedat Tolga; Kavukcu, Serdar Batikan; Caglar, Ayse Dila; Nalbantsoy, Ayse; Turkmen, Hayati
This investigation scrutinizes the cytotoxic properties inherent in nitron metal complexes, a commercially available 1,2,4-triazole derivative. The study assesses the anticancer potential of ruthenium(II) (1), rhodium(III) (2), iridium(I/III) (3 and 5), palladium(II) (4), and platinum(II) (6) complexes of nitron by subjecting them to examination as agents against cancer cell lines, namely HeLa, MDA-MB-231, PC3, and non-cancerous CCD-34Lu cells. Results derived from the MTT assay substantiate that each nitron complex manifests cytotoxic activity against a spectrum of cancer cells. Notably, the ruthenium (Ru) and rhodium (Rh) complexes exhibit pronounced cytotoxic effects on cancer cells, suggesting their potential as impactful chemotherapeutic agents. Flow cytometry studies further reveal a predilection of these complexes towards inducing early apoptosis in the target cells. The investigation extends to exploring the stability and aquation dynamics of complex (1), in addition to elucidating the binding model between complex (1) and DNA.
Antimicrobial and antioxidant activities with acute toxicity, cytotoxicity and mutagenicity of Cystoseira compressa (Esper) Gerloff & Nizamuddin from the coast of Urla (Izmir, Turkey)
(Springer, 2015) Guner, Adem; Koksal, Cinel; Erel, Sura Baykan; Kayalar, Husniye; Nalbantsoy, Ayse; Sukatar, Atakan; Yavasoglu, N. Ulku Karabay
The aim of the study was to evaluate the biological activities with toxic properties of the methanol, hexane, and chloroform extracts of Cystoseira compressa (Esper) Gerloff & Nizamuddin from the Coast of Urla in the Aegean Sea. The extracts of C. compressa were tested for their antimicrobial and antioxidant activities in this study. Cytotoxic and mutagenic potentials of the extracts were also evaluated using cell culture and mutagenicity assays. Hexane extract was found to have higher total flavonoid and phenolic contents than the other extracts and exerted higher antioxidant activity than other extracts. All extracts exhibited moderate antimicrobial activity against tested microorganisms (minimum inhibitory concentration ranges are 32-256 mu g/mL). The results indicated that the extracts had no significant cytotoxic activity against human hepatocellular carcinoma Hep 3B cell line in all treated concentrations (5-50 mu g/mL) and did not show mutagenicity in the Ames test. Lethality was not observed among mice treated with oral doses of the extracts. In conclusion, results of investigations indicate that brown alga C. compressa is a natural source of antioxidant. It has moderate antimicrobial activities with no toxicity.
Antioxidant, cytotoxic and anti-inflammatory properties of Anthemis tricolor Boiss. through a series of cellular assays and inhibition of Turkish Macrovipera lebetina obtusa venom induced inflammation in rat
(Istanbul Univ, Fac Pharmacy, 2023) Askin, Dilara; Akbulut, Tugce Demiroz; Baykan, Sura; Gocmen, Bayram; Khan, Shabana; Kirmizibayrak, Petek Ballar; Nalbantsoy, Ayse
Background and Aims: Venom of Macrovipera lebetina obtusa (Viperidae), Turkey's biggest viper, is highly toxic to humans and causes inflammation. In Anatolian traditional medicine, manyAnthemis L. species are used in wound healing and against inflammatory diseases. In the present study, antioxidant, cytotoxic and anti-inflammatory activities of different endemic A. tricolor Boiss. extracts were evaluated by in vitro assays. Besides, the anti-inflammatory activities of chloroform extract against carrageenan and snake venom induced-edema were investigated in rats. Methods: Antioxidant and anti-inflammatory activities were evaluated by DCFH-DA, iNOS, NF-kappa B inhibitions and NAG-1 gene activation, while WST-1 assay was preferred for cytotoxic activity. Besides, the hind paw edema test was used for in vivo studies. Results: Chloroform extract exhibited the strongest NAG-1-inducing activity. In addition, this extract showed potent iNOS and NF-kappa B inhibition (IC50:14.0 and 10.75 mu g/mL, respectively) and cytotoxic effect against human osteosarcoma (U2OS; IC50: 15.18 mu g/mL) and human cervical cancer (HeLa; IC50: 18.3 mu g/mL) cell lines. Moreover, chloroform extract had stronger anti-inflammatory effects against both carrageenan and snake venom induced-edema formation than indomethacin. Fifty and 100 mg/kg extracts reduced the paw edema to 1-3% at 4 h after the snake venom injection. On the other hand, 50, 25 and 12.5 mg/kg of the extract completely inhibited inflammation induced by carrageenan.Conclusion: This is the first report on the antioxidant, cytotoxic and anti-inflammatory effects of endemic A. tricolor by in vitro studies and snake venom-induced paw edema in rats. The plant exhibits strong potential for treating local tissue damage in snake bites.
Antioxidant, cytotoxic and iNOS activity of Liquidambar orientalis Mill. resin extracts
(Walter De Gruyter Gmbh, 2016) Nalbantsoy, Ayse; Karis, Mert; Karakaya, Leyla; Akgul, Yurdanur
Objective: The aim of this study is to investigate the in vitro cytotoxicity and iNOS (inducible nitric oxide synthase) inhibitory, and antioxidant activity in order to assess the traditional usage of Liquidambar orientalis Mill resin extract. Methods: Different solvent extracts of Liquidambar orientalis Mill resin were prepared. The cytotoxicity of extracts was determined using MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide) assay. HeLa (Human cervix adenocarcinoma), A-549 (Human alveolar adenocarcinoma), MCF-7 (Human breast adenocarcinoma), CaCo-2 (Human colon colorectal adenocarcinoma), mPANC96 (Human pancreas adenocarcinoma), PC-3 (Human prostate adenocarcinoma), U87MG (Human glioblastoma-astrocytoma) and as a normal cell line HEK293 (Human embryonic kidney cells) and Vero (African green monkey kidney epithelial cells) were used for testing cytotoxicity. RAW 264.7 (murine macrophage cell lines) was used to determine the inhibition levels of inducible nitric oxide synthase (iNOS). HL-60 (human acute myeloid leukemia) was used to determine antioxidant activity as DCF production per cent. Results: Hexane, dichloromethane, methanol and water extracts were prepared, and their iNOS inhibitory, cytotoxic and antioxidant activity were investigated. The estimated IC 50 values of extracts varied from 6.68 to 48.90 mu g/ml after treatment with different doses of extracts for 48 h. Inhibition of the hexane, dichloromethane, methanol, and water extracts on LPS-induced NO production in RAW 264.7 macrophage were showed that the all extracts inhibited NO production in activated RAW 264.7 cells, except methanol extract. Hexane, dichloromethane and methanol extracts inhibited NO production with IC 50 values of 22 mu g/ml, 22 mu g/ml, and 21/ml mu g, respectively. The antioxidant activity was determined by the production of DCF in HL-60 cells. The extracts showed a potent antioxidant effect, with an IC 50 of in between from 19.01 to 39.77 mu g/ml. Conclusion: This study is the first report showing the potential of Liquidambar orientalis Mill resin extracts for cytotoxicity, iNOS inhibition and the antioxidant activity as an alternative therapeutic approach for traditional uses. The results demonstrated that Liquidambar orientalis dichloromethane resin extracts showed strongest cytotoxic effect while all extracts except methanolic extracts exhibited moderate iNOS inhibition. All extracts other than hexane have a potent antioxidant effect in the cellular-based assay. In conclusion, further studies should focus on the purification of the active components of this extracts and to investigate the possible mode of action to obtain a better understanding of their potential use as cytotoxic, anti-inflammatory and antioxidant agents.
Apoptosis-inducing activities of Halopteris scoparia L. Sauvageau (Brown algae) on cancer cells and its biosafety and antioxidant properties
(Springer, 2019) Guner, Adem; Nalbantsoy, Ayse; Sukatar, Atakan; Yavasoglu, Nefise Ulku Karabay
The aim of this study was to reveal the biological activities and in vivo toxicity profiles of n-hexane, chloroform and methanol extracts of brown algae Halopteris scoparia L. Sauvageau. In this study, extracts were tested for their phytochemical contents and antioxidant activities. The cytotoxic activities of the extracts against cervical adenocarcinoma (HeLa), colon colorectal adenocarcinoma (CaCo-2) and breast adenocarcinoma (MCF7) cells were assessed by MTT assay and total RNAs derived from cell lines to analyze gene expression were analyzed by Real Time Ready Human Apoptosis Panel 96. Also, in vivo toxicity and irritation effects of extracts were evaluated by LD50 acute toxicity test and Hen's egg test chorioallantoic membrane (HET-CAM) assay, respectively. Our results showed that the phenolic and flavonoid contents were determined only in methanol extract (33.20 +/- 1.41mg GAE/g and 1.26 +/- 0.95mg QE/g). Also, n-hexane has a broader spectrum of content than methanol and chloroform extracts. Furthermore, n-hexane extract in DPPH and methanol extract in ABTS(+) exhibited the best antioxidant activity. In addition, MTT results revealed that each three extracts cause a significant reduction in cell viability, especially in HeLa cells. When the apoptotic gene expressions were examined after treatment of extracts, the expression of many pro-apoptotic genes in both caspase-independent and caspase-dependent intrinsic and extrinsic pathways increased. These findings suggest that, considering that it had not led to irritation and toxicity in vivo, edible H. scoparia is a natural antioxidant and its apoptotic/cytotoxic activities can potentially be used against human cancers.
Apoptosis-inducing activity of safflower (Carthamus tinctorius L.) seed oil in lung, colorectal and cervix cancer cells
(Springer, 2020) Guner, Adem; Kizilsahin, Sadi; Nalbantsoy, Ayse; Yavasoglu, Nefise Ulku Karabay
Carthamus tinctorius L. (Safflower) has been often preferred because of rich fatty acid, flavonoid, alkaloid, and polysaccharide contents in its different parts in medicine and industrial area. Although its antioxidant, antienflamatuar, and antitumor properties have been proven in many studies, the mechanism underlying the anticancer activity is still more unclear. This study was first conducted to elucidate the apoptotic gene expression changes in human colorectal (CaCo-2), lung (A549), and cervix cancer (HeLa) cells after exposure to safflower seed oil (SFO). Cytotoxic activity of cancer cells was evaluated by MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl2H-tetrazoliumbromide) assay and then, total RNA derived from cell lines to analyze the gene expression profile on Real-Time Ready Human Apoptosis Panel 96 was used. MTT results showed that SFO greatly inhibited A549, CaCo-2 and HeLa cell proliferation, with a value of IC50 of 1.26, 3.92 and 13.12 mu g/ml, respectively. According to the cDNA microarray analysis, 56 genes were interpreted in connection with extrinsic, intrinsic, PI3K/AKT, JAK/STAT, and NF kappa B pathways. SFO treatments triggered apoptosis through the caspase-dependently pathway along with upregulated the expressions of many pro-apoptotic genes in the extrinsic and intrinsic pathway in HeLa cells. However, in A549 and CaCo-2 cells, SFO treatments were inhibited cell survival mechanism through frequently caspase-independent genes following downregulated the expression of anti-apoptotic genes. It is noteworthy that although cancer cells have different sensitivity, SFO induced apoptosis through different pathways. Taken together, SFO, as a natural resource, has the potential to be used as a promising agent against cancer, especially in gene therapy level.
Assessment of in vitro Cytotoxic, iNOS, Antioxidant and Photodynamic Antimicrobial Activities of Water-soluble Sulfonated Phthalocyanines
(Wiley, 2022) Akin, Mustafa; Saki, Neslihan; Guzel, Emre; Orman, Batuhan; Nalbantsoy, Ayse; Kocak, Makbule B.
In recent years, much effort has been devoted to the development of effective anticancer agents. In this manner, the utilization of water-soluble sulfonated phthalocyanines is crucial for many cancer cell lines. In this study, phthalonitrile and metallophthalocyanine compounds linked by benzenesulfonic acid groups have been prepared. Antimicrobial behaviors of those compounds were investigated by performing disk diffusion and photodynamic assays on gram-positive and negative bacteria. Indium phthalocyanine (InClPc) (3) showed inhibition activity against B. cereus, B. subtilis and S. aureus with disk diffusion assay. Also, gallium and indium phthalocyanines (2 and 3) exhibited inhibitory activity on both gram-positive and -negative microorganisms after light activation. Increasing the inhibitor concentration and light exposure time increased the inhibition activity for both molecules. GaClPc (2) demonstrated the maximum reducing power capacity among studied compounds, and CoPc (4) showed even better DPPH radical scavenging ability than the standard molecule Trolox at 2000 mu g mL(-1) concentration. The dose-dependent effect of compounds on cytotoxicity was studied against cancer cells PANC-1, MDA-MB-231, HepG2, A549, HeLa, CaCo-2 and non-tumorigenic cells HEK-293. All compounds showed no significant cytotoxic effect on any cell line up to the highest treated concentration at 50 mu g mL(-1). However, all phthalocyanines had significant nitric oxide inhibition activity, and only in copper phthalocyanine (CuPc) (5), the MTT IC50 value was reached on LPS-activated RAW 264.7 macrophage cells. The lowest inducible nitric oxide synthase (iNOS) IC50 values were defined as 6 +/- 1 mu g mL(-1) and 7 +/- 0.5 mu g mL(-1) for CuPc (5) and InClPc (3), respectively.
Assessment of probiotic, biosafety and technological properties of lactic acid bacteria isolated from tarhana
(Springer, 2024) Atlama, Kivanc; Sengun, Ilkin Yucel; Nalbantsoy, Ayse
This research investigated the probiotic potential of lactic acid bacteria isolated from tarhana, a traditional Turkish food. In this context, the probiotic, biosafety and technological properties of the strains previously identified as Enterococcus faecium, Lacticaseibacillus casei, Lactiplantibacillus plantarum, Leuconostoc citreum, L. mesenteroides, L. pseudomesenteroides, Limosilactibacillus fermentum, Pediococcus acidilactici, P. pentosaceus, Streptococcus thermophilus and Weissella cibaria were tested. All strains (except W. cibaria IST29) demonstrated tolerance to bile salts (83.78-115.30%), pepsin (65.27-126.52%), pancreatin (72.66-106.15%), and phenol (67.70-115.81%) at various levels. None of them (except L. plantarum IST7) showed hemolytic activity. The strains demonstrated different levels of sensitivity to the tested antibiotics. The proteolytic activity of the strains varied between 0.011 and 0.067 mg tyrosine/mL. The strains also showed antimicrobial activity (inhibition zone between 7 and 30 mm) against test microorganisms. The isolates showing the best gastrointestinal tolerance were tested for their ability to produce short-chain fatty acids and produced acetate, butyrate and propionate in the range of 11945-17715, 0.0580-0.1110 and 14335-22650 mu g/mL, respectively. The auto-aggregation and co-aggregation values of the strains ranged from 7.51 to 55.86% and 0.34-62.09%, respectively, while their xylene and ethyl acetate hydrophobicity were in the range of 69.40-91.81% and 72.36-95.21%, respectively. Their CaCo-2 cell adhesions ranged from 0.233 to 12.347%. Consequently, L. citreum IST9, L. fermentum IST13, L. fermentum IST14, and P. pentosaceus IST21 were identified as the most promising probiotic strains.
Bioactivity screening of five Centaurea species and in vivo anti-inflammatory activity of C. athoa
(Taylor & Francis Ltd, 2014) Erel, Sura Baykan; Demir, Serdar; Nalbantsoy, Ayse; Ballar, Petek; Khan, Shabana; Yavasoglu, N. Ulku Karabay; Karaalp, Canan
Context: Centaurea L. (Asteraceae) species used as herbal remedies in Turkish traditional medicine have shown several biological properties. Objective: Extracts obtained from the aerial parts of Centaurea aphrodisea Boiss., Centaurea athoa DC., Centaurea hyalolepis Boiss., Centaurea iberica Trev. and Centaurea polyclada DC. were evaluated for their antioxidant, cytotoxic and anti-inflammatory activities. Materials and methods: Extracts of Centaurea species were tested for their antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) screening assays and for in vitro anti-inflammatory activity by Nf-kappa B and iNOS inhibition assays. The extracts were tested for their in vitro cytotoxicities against a panel of human solid tumor cell lines (SK-MEL: malignant melanoma, KB: oral epidermal carcinoma, BT-549: breast ductal carcinoma and SK-OV-3: ovary carcinoma) as well as non-cancerous kidney fibroblast (Vero) and kidney epithelial cells (LLC-PK1) by Neutral Red assay. In vivo anti-inflammatory activity of C. athoa was evaluated by the carrageenan-induced paw edema test in rats. Results: Antioxidant activities were observed for methanol extracts of plants. C. polyclada had the strongest effect on BT-549, KB and SK-OV-3 cell lines (30, 33 and 47 mu g/ml, respectively). Nf-kappa B inhibition of chloroform extract of C. athoa was determined equivalent to positive control parthenolide (IC50: 6 mu g/ml). This extract also showed anti-inflammatory activity by the carrageenan-induced paw edema test in rats, in all hours at a dose of 50 mg/kg compared to the control group. Discussion and conclusion: C. athoa is suggested to be a potential source of lead compounds for inflammatory diseases due to the significant in vitro and in vivo anti-inflammatory results.
Bioassay-guided isolation and identification of Aedes aegypti larvicidal and biting deterrent compounds from Veratrum lobelianum
(De Gruyter Poland Sp Zoo, 2018) Tabanca, Nurhayat; Ali, Zulfiqar; Bernier, Ulrich R.; Epsky, Nancy; Nalbantsoy, Ayse; Khan, Ikhlas A.; Ali, Abbas
An ethanol extract from Veratrum lobelianum Bernh. rhizomes showed larvicidal activity with LC50 values of 11.79 ppm and 89.9 ppm against rand 4 1 b instar larvae, respectively, at 24 h post-treatment. The extract also showed proportion not biting (PNB) value of 0.76 at 100 mu g/cm(2) against females of Ae. aegypti. Systematic bioassay-guided fractionation of V. lobelianum extract resulted in the isolation of five compounds that were identified as ethyl palmitate (1), ethyl linoleate (2), p-sitosterol (3), resveratrol (4) and oxyresveratrol (5) by GC-MS, H-1-NMR, and C-13-NMR techniques, comparison with literature data, and confirmation with authentic compounds. Compound 2 exhibited larvicidal activity with an LC50 value of 24.1 (22.0-26.2) ppm whereas 1 was inactive. B-Sitosterol (3) displayed the highest larvicidal activity with LC50 = 1.7 (1.3-2.3) ppm and LC90 = 5.1 (3.4-13.8) ppm. Compounds 4 and 5 had larvicidal activity with LC50 values of 18.5 (15.3-22.3) and 22.6 (19.0-26.8) ppm, respectively, and had an equivalent PNB values of 0.75 at 25 nmol/cm(2). In addition, 4 and 5 were explored for their human-based repellency against Ae. aegypti, attractiveness against male medflies Ceratitis capitata, and also evaluated against series of human carcinoma cells (A549, HEK293, HeLa, SH-SY5Y); however, no significant activity was found.
Biological activities of skin and parotoid gland secretions of bufonid toads (Bufo bufo, Bufo verrucosissimus and Bufotes variabilis) from Turkey
(Elsevier France-Editions Scientifiques Medicales Elsevier, 2016) Nalbantsoy, Ayse; Karis, Mert; Yalcin, Husniye Tansel; Gocmen, Bayram
Toad glandular secretions and skin extractions contain numerous natural agents which may provide unique resources for novel drug development. Especially the skin-parotoid gland secretions of toads from genus Bufo contain as many as 86 different types of active compounds, each with the potential of becoming a potent drug. In the present study, crude skin-parotoid gland secretions from Bufo bufo, Bufo verrucosissimus and Bufotes variabilis from Turkey were screened against various cancer cells together with normal cells using MTT assay. Furthermore, the antimicrobial properties of skin secretions were tested on selected bacterial and fungal species for assessing the possible medical applications. Antimicrobial activity of skin secretions was studied by determining minimal inhibitory concentration ( MIC) in broth dilution method. Hemolytic activity of each skin-secretion was also estimated for evaluating pharmaceutical potential. Both skin-parotoid gland secretions showed high cytotoxic effect on all cancerous and non-cancerous cell lines with IC50 values varying between <0.1 mu g/ml and 6.02 mu g/ml. MIC results of antimicrobial activity tests were found to be between 3.9 mu g/ml and 250 mu g/ml. No hemolytic activities on rabbit red blood cells at concentrations between 0.5 mu g/ml and 50 mu g/ml were observed. In conclusion, skin-parotoid secretions of bufonid toads might be remarkable candidates for anti-cancer and antimicrobial agents without hemolytic activities. (C) 2016 Elsevier Masson SAS. All rights reserved.
Chemical Characterization and Biological Activity of the Mastic Gum Essential Oils of Pistacia lentiscus var. chia from Turkey
(Mdpi, 2020) Tabanca, Nurhayat; Nalbantsoy, Ayse; Kendra, Paul E.; Demirci, Fatih; Demirci, Betul
The essential oils (EOs) were isolated by hydrodistillation from wild and cultivated Pistacia lentiscus L. var. chia-mastic gum tree (Anacardiaceae) from two natural habitats, namely from Cesme-Uzunkoy (1) and Mordogan (2), and one cultivated source, Cesme-Germiyan (3), in Izmir, Turkey. This comparative study evaluated the chemical composition and biological activity of mastic gum essential oils (MGEOs). For this purpose, MGEOs 1-3 were analyzed by gas chromatography-flame ionization detection (GC-FID), gas chromatography-mass spectrometry (GC-MS), and chiral GC for alpha-pinene. Laboratory assays were conducted to assess for potential in vitro cytotoxicity (multiple in vitro cancer cell lines), antimicrobial properties (five bacterial species and yeast), anti-inflammatory activity (inhibition of inducible nitric oxide synthase, iNOS), and the attraction of Ceratitis capitata (Mediterranean fruit fly, medfly), respectively. Chemical analysis indicated that MGEOs 1 and 2 were rich in alpha-pinene (56.2% and 51.9%), myrcene (20.1% and 18.6%), and beta-pinene (2.7% and 3.1%), respectively; whereas MGEO-3 was characterized by a high level of alpha-pinene (70.8%), followed by beta-pinene (5.7%) and myrcene (2.5%). Chiral GC analyses showed that concentration ratios between (-)/(+)-alpha-pinene and (-)-alpha-pinene/myrcene allowed for differentiation between wild and cultivated MGEO sources. in biological assays, MGEOs 1-3 did not exhibit significant antimicrobial effects against the pathogens evaluated and were not strong attractants of male medflies; however, all three MGEOs displayed a dose-dependent inhibition of iNOS, and MGEOs 1 and 2 exhibited selective in vitro cytotoxicity against human cancer cells. These results suggest that wild-type mastic gum oils from Cesme and Mordogan (MGEOs 1 and 2) are potential sources of beneficial products and warrant further investigation.
Chemical composition and biological activities of Cypriot propolis
(Taylor & Francis Ltd, 2022) Nalbantsoy, Ayse; Sarikahya, Nazli Boke; Ozverel, Cenk Serhan; Barlas, Ayse Bercin; Kirci, Damla; Akgun, Ismail Hakki; Yalcin, Tansel
Propolis compositions are highly variable, depending on the geographic region and the season of collection. In this study, propolis samples from seven different regions of Cyprus were studied for the first time by means of chemical content and biological activities. Secondary metabolite composition was determined by LC-HRMS. While the major flavonoids found were isosakuranetin, naringenin, rhamnocitrin, diosmetin, chrysin and acacetin, interestingly verbascoside, a phenylethanoid glycoside, and chlorogenic acid were identified as the major compounds in the ethanol-water extracts. alpha-Pinene was detected as the major compound of propolis extracts according to the volatile compositions via GC-MS. Karaoglanoglu and Tirmen extracts, presenting different chemical profiles, exerted enormous cytotoxic activity by MTT assay (IC50: 2.36-11.56 mu g/mL; 1.44-9.33 mu g/mL, respectively). The highest iNOS inhibition potential was detected in the Karpaz extract (IC50:2.6 mu g/mL) in LPS induced RAW 264.7 cells whereas the Guzelyurt sample demonstrated remarkable antioxidant (88.82 +/- 0.10%) and antimicrobial activities (with a MIC value of 31.2 mu g/mL against S. aureus, S. epidermidis, E. faecium, and E. faecalis).
Chemical constituents and biological activities of Dianthus elegans var. elegans
(Taylor & Francis Ltd, 2018) Mutlu, Kiymet; Sarikahya, Nazli Boke; Nalbantsoy, Ayse; Kirmizigul, Suheyla
Chemical investigation of the aerial parts of Dianthus elegans var. elegans afforded two previously undescribed saponins, named dianosides M-N (1-2), together with four oleanane-type triterpenoid glycosides (3-6). Their structures were elucidated as 3-O--L-arabinofuranosyl-16-hydroxyolean-12-ene-23, 28-dioic acid (1) and 3-O--L-arabinofuranosyl-(13)--D-glucopyranosyl 16-hydroxyolean-12-ene-23-oic acid, 28-O--D-glucopyranosyl-(16)--D-glycosyl ester (2) by chemical and extensive spectroscopic methods including IR, 1D, 2D NMR and HRESIMS. Both of the saponins were evaluated for their cytotoxicities against HEK-293, A-549 and HeLa human cancer cells using the MTT method. All compounds showed no substantial cytotoxic activity against tested cell lines. However, dianosides M-N and the n-butanol fraction exhibited considerable haemolysis in human erythrocyte cells. The immunomodulatory properties of dianosides M-N were also evaluated in activated whole blood cells by PMA plus ionomycin. Dianosides M-N increased IL-1 concentration significantly whereas the n-butanol fraction slightly augmented IL-1 secretion. All compounds did not change IL-2 and IFN- levels considerably. [GRAPHICS]
Chemical constituents and in vitro biological activities of Eremurus spectabilis leaves
(Taylor & Francis Ltd, 2017) Karakaya, Leyla; Akgul, Yurdanur; Nalbantsoy, Ayse
In the present study, phytochemical investigation of the young leaves of Eremurus spectabilis let to the isolation six compounds (1-6). Their structure was determined by spectroscopic analysis and comparison with the literature data. All compounds were isolated for the first time from E. spectabilis. Isolated compounds and prepared extracts did not show cytotoxic activity against HeLa (Human cervix adenocarcinoma), A-549 (Human alveolar adenocarcinoma), MCF-7 (Human breast adenocarcinoma), mPANC96 (Human pancreas adenocarcinoma), U87MG (Glioblastoma), PC3 (Prostate), CaCo-2 (Colon), HEK293 (Human embryonic kidney cells) cell lines. RAW 264.7 (murine macrophage cell lines) was used to determine the inhibition levels of inducible nitric oxide synthase (iNOS).The hexane extract was found to be active in terms of inhibition of iNOS (IC50: 25g/ml). [GRAPHICS] .

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