Anti-inflammatory and antioxidant properties of betaine protect against sepsis-induced acute lung injury: CT and histological evidence
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Associacao Brasileira de Divulgacao Cientifica
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this research was to determine the anti-inflammatory effect of betaine on sepsis-induced acute respiratory distress syndrome (ARDS) in rats through histopathological examination, radiologic imaging, and biochemical analysis. Eight rats were included in the control group, and no procedure was performed. Feces intraperitoneal procedure (FIP) was performed on 24 rats to create a sepsis-induced ARDS model. These rats were separated into three groups as follows: FIP alone (sepsis group, n=8), FIP + saline (1 mL/kg, placebo group, n=8), and FIP + betaine (500 mg/kg, n=8). Computed tomography (CT) was performed after FIP, and the Hounsfield units (HU) value of the lungs was measured. The plasma levels of tumor necrosis factor (TNF)-a, interleukin-1b (IL-1b), IL-6, C-reactive protein, malondialdehyde (MDA), and lactic acid (LA) were determined, and arterial oxygen pressure (PaO2) and arterial CO2 pressure (PaCO2) were measured from an arterial blood sample. Histopathology was used to evaluate lung damage. This study completed all histopathological and biochemical evaluations in 3 months. All evaluated biomarkers were decreased in the FIP + betaine group compared to FIP + saline and FIP alone (all P<0.05). Also, the parenchymal density of the rat lung on CT and histopathological scores were increased in FIP + saline and FIP alone compared to control and these findings were reversed by betaine treatment (all P<0.05). Our study demonstrated that betaine suppressed the inflammation and ameliorated acute lung injury in a rat model of sepsis. © 2023, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.
Açıklama
Anahtar Kelimeler
Acute lung injury, Betaine, Rat model, Sepsis, betaine, C reactive protein, interleukin 1beta, interleukin 6, lactic acid, malonaldehyde, tumor necrosis factor, antiinflammatory agent, antioxidant, betaine, tumor necrosis factor, animal experiment, animal model, animal tissue, antiinflammatory activity, antioxidant activity, arterial carbon dioxide tension, arterial oxygen tension, Article, biochemistry, blood sampling, cardiac puncture, computer assisted tomography, controlled study, enzyme linked immunosorbent assay, histopathology, immunohistochemistry, lung injury, nonhuman, rat, sepsis-induced acute lung injury, acute lung injury, animal, complication, lung, lung injury, pathology, respiratory distress syndrome, sepsis, Sprague Dawley rat, x-ray computed tomography, Acute Lung Injury, Animals, Anti-Inflammatory Agents, Antioxidants, Betaine, Lung, Lung Injury, Rats, Rats, Sprague-Dawley, Respiratory Distress Syndrome, Sepsis, Tomography, X-Ray Computed, Tumor Necrosis Factor-alpha
Kaynak
Brazilian Journal of Medical and Biological Research
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
56
