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Öğe Developing an interprofessional identity complementary to a professional identity - findings related to Extended Professional Identity Theory (EPIT)(Frontiers Media SA, 2024) Reinders, Jan Jaap; Baser Kolcu, Mukadder Inci; Kolcu, GirayCollaboration among various professions often faces barriers owing to divergent perspectives, priorities, and expertise shaped by distinct socialization processes. These differences can hinder effectiveness, efficiency, and workforce well-being. The Extended Professional Identity Theory (EPIT) addresses this issue by fostering an interprofessional identity without weakening professional identities. Drawing from psychological theories, EPIT explains the coexistence of interprofessional and professional identities, and predicts associated behaviors. It also emphasizes the importance of combining interprofessional identity formation with developing interprofessional competencies and adapting to environmental factors to achieve synergy in (temporary or permanent) mixed profession groups. Introduced in 2018, EPIT research initially relied on the measurement of congruent interprofessional behaviors as indirect indicators of interprofessional identity that could not yet be measured. An experiment demonstrated that enhancing social identification in mixed profession groups with interprofessional assignments reduced the social hierarchy within 6 h across three meetings. The 2020 development of the Extended Professional Identity Scale (EPIS) confirmed interprofessional identity as a three-dimensional social construct. So far, several scientific studies have supported many propositions of EPIT. These propositions are related to dimensionality and various psychometric properties, cross-cultural similarities, evidence and clues for interprofessional identity formation, and its predictive validity in interprofessional education and collaborative practice. T & uuml;rkiye is among several countries (e.g., the Netherlands, Belgium, Germany, Lithuania, Finland, and Indonesia) where EPIT-based interprofessional identity is being investigated. To illustrate contextual differences and their potential cross-cultural implications, it is valuable to explore how interprofessional identity adds value in the Turkish context. This approach facilitates understanding the regional implications of interprofessional identity, including interprofessional education initiatives, increased university engagement, the development of measurement instruments, challenges and future directions, and national and international collaborations. This paper aims to explain and clarify EPIT propositions compared to other theories, describe current evidence, and outline future research directions, with a focus on developments within the Turkish context as a showcase.Öğe Comparative analysis of protein profiles in skin secretions of some Rana species: Preliminary insights into antimicrobial activity(Pergamon-Elsevier Science Ltd, 2024) Tanriverdi, O. Ebru; Ayaz, Dincer; Terzi, YigitProtein profiles of skin secretions of Rana dalmatina (Agile Frog), Rana macrocnemis (Uludag Frog), Rana tavasensis (Tavas Frog) and Rana holtzi (Taurus Frog) frog species belonging to the Rana genus distributed in the Anatolian region of Turkiye were determined for the first time using the Tricine-SDS-PAGE Electrophoresis method and Coomassie Brilliant Blue (CBB) staining. By the results, some peptides with mass <5 kDa were detected. Just one peptide with mass <5 kDa was found in the secretion of each R. dalmatina, R. macrocnemis, and R. tavasensis while there was two in R. holtzi secretion. The antibacterial activity of secretions was determined using plate well diffusion assay on E. coli, S. typhimurium, S. aureus, B. cereus and L. monocytogenes bacteria. R. dalmatina created the inhibition zone for S. typhimurium, S. aureus, B. cereus, and L. monocytogenes. The zones of inhibition by R. tavasensis and R. macrocnemis species secretions were observed on S. aureus, B. cereus, and L. monocytogenes. It was found that R. holtzi creates an inhibition zone only on B. cereus. The results showed that the secretion of none of the species doesn't have antibacterial activity on E. coli. The skin secretion of R. dalmatina showed the most activity against bacteria, while R. holtzi had the least.Öğe In Vitro Antagonistic Activity of Plant Growth Promoting Rhizobacteria Against Aggressive Biotypes of the Green Mold(Wiley, 2024) Mis, Baran; Karaca, Kemal; Eltem, RenginDuring the cultivation of button mushrooms, the green mold epidemic, which causes a decrease in productivity, is a very important problem. The environmental harm of chemicals used in the control of such epidemics and the demand of consumers for organic products without chemicals have brought environmentally friendly biological control to the fore. Biological control can be achieved by the use of antagonistic microorganisms and their metabolites. In this study, the effectiveness of Bacillus spp. and Pseudomonas spp. for the biological control of the aggressive biotypes of the green mold disease agent Trichoderma aggressivum strains was examined in vitro. For this purpose, the antifungal effects of Bacillus spp. and Pseudomonas spp. against T. aggressivum strains were examined by in vitro dual culture test. Afterward, the antifungal activity of Bacillus spp. metabolites was assessed further using the agar well diffusion method. Then, it was determined whether the bacterial strains showing antifungal activity showed antagonistic activity against A. bisporus. Although none of the Pseudomonas spp. showed antifungal activity against T. aggressivum strains, most of the Bacillus spp. were found to have high activity. It has been concluded that Bacillus sp. & Ouml;-4-82 may be potential biological control agent for button mushroom cultivation.Öğe Psychometric properties of the Turkish version of the developmental care scale for neonates with congenital heart disease(Cambridge University Press, 2024) Seckin Kolak, Sevgi; Kahraman, AyseObjective: Developmental care for newborns with congenital heart disease (CHD) improves cardiac and respiratory patterns. According to the American Heart Association, developmental care in newborns with CHD is important for improving neurodevelopmental outcomes. This study aimed to evaluate the validity and reliability of the Turkish version of the Developmental Care Scale for Neonates with Congenital Heart Disease. Methods: This was a methodological, descriptive study conducted with 169 nurses from a tertiary-level NICU. The Demographical Information Form and the Developmental Care Scale for Neonates with Congenital Heart Disease were used to collect the data. The scales’ language and content validity, construct validity, and internal consistency were also assessed. Results: The scale consists of 31 items and four subscales. Factor loadings ranged from 0.44 to 0.82 and explained 65% of the total variance. Fit indices indicate that the model is acceptable. Cronbach’s α was 0.95 for the entire instrument, 0.91 for developing the external environment subscale, 0.94 for assessing family well-being, 0.86 for the caregiver activities toward the neonate, and 0.82 for the basic need subscale. Item–total correlations ranged between 0.34 and 0.75, according to the item analysis results. Conclusions: The Turkish version of the Developmental Care Scale for Neonates with Congenital Heart Disease is valid and reliable. The use of this scale could improve the performance of neonatal intensive care nurses in providing developmental care to newborns with CHD as well as the quality of care.Öğe Deep masseteric triangular area to define masseter neurovascular bundle: A cadaveric study(Elsevier, 2024) Coban, Istemihan; Yaprak, Fulya; Pinar, YeldaIntroduction: Facial reanimation procedures are used in the treatment of the disorder that impairs mimetic function and jeopardizes physical and psychological health, and one of the most important instruments of these techniques is the masseteric neurovascular bundle (NVB) and proper identification at the mandibular notch level. In the current study, a triangular area (deep masseteric triangle, DMT) on the lateral surface of the masseter muscle that was identified to help reliable determination of the masseteric NVB at the mandibular notch level. Material and methods: 40 parotideomasseteric region dissections were performed in 10 female and 10 male donated cadavers. Structures lateral to the masseter muscle were removed. The edge length of the masseter muscle on the zygomatic arch side was measured. After the edges of the DMT were measured, the masseteric NVB was found by dissection and its distance (depth) from the skin line was measured. Results: The mean lengths of the superior, posterior, and anterior margins were 17.3 (±4.5) mm, 25.9 (±6.2) mm, and 26.3 (±6.5) mm, respectively. The total length of the upper edge of the masseteric muscle attached to the zygomatic arch averaged 52.7 (±5.2) mm. The masseteric neurovascular bundle was detected at a depth of approximately 17 mm from the skin of the parotideamasseteric region. Discussion: The visualization of the DMT can be used as an important landmark for access to branch-free part of the masseteric nerve. Moreover, an specific approach for masseteric NVB localization can be established by drawing a line between the mandibular angle and the midpoint of the upper edge of the DMT. This technique can greatly improve the accuracy of both masseteric nerve harvesting and masseteric nerve block procedures.Öğe Effectiveness of surgical approach in the management of non-traumatic corneal perforations(Taylor and Francis Ltd, 2024) Furundaoturan, Onur; Palamar, Melis; Egrilmez, Sait; Yagci, Ayse; Barut Selver, OzlemClinical relevance: Non-traumatic aetiologies are one of the leading causes of corneal perforations. The management of corneal perforation is quite challenging and complex for anterior segment surgeons. The appropriate surgical approach for each case is usually determined on the basis of a combination of many different parameters. Background: The study aimed to evaluate surgical approach options and outcomes in the treatment of non-traumatic corneal perforations. Methods: Patient data who underwent surgery for non-traumatic corneal perforation between 2016 and 2023 were retrospectively evaluated. Medical records were assessed in terms of age, gender, perforation aetiology, the first and last examination notes, surgical approach, follow-up time, and additional surgeries and outcomes according to anatomical, therapeutic and functional success. Anterior segment photographs were investigated for thorough explanation of the examinations. Results: Forty-five eyes of 45 patients were included (mean age 61.2 ± 22.4 (90-2), female/male ratio 20/25). Surgical approaches applied according to the size and location of the perforation site included fibrin glue application (6), amniotic membrane transplantation (AMT) (9), corneal patch graft application(15), and tectonic keratoplasty (15). The ratio of inflammatory and infectious causes as the two main indications was 29/16. Globe integrity was ensured with the first surgery in 27 eyes. However, 17 eyes required secondary surgical attempts due to failure of the first approach and 1 eye underwent evisceration. AMT was the least successful method among other methods in anatomical, therapeutic, and functional assessment. Conclusion: There are various surgical approaches for repairing non-traumatic corneal perforations, each with its own advantages and disadvantages. These include high tissue resistance, the ability to remove necrotic tissue, ease of access, and anti-inflammatory activity. It is possible to successfully repair corneal perforations with single and combined methods, considering the above-mentioned features, especially depending on the size and location of the defect. While AMT is a viable and time-saving choice − especially in the lack of donor tissues − further interventions are necessary in most circumstances.Öğe A machine learning approach to predict foot care self-management in older adults with diabetes(BMC, 2024) Ozgur, Su; Mum, Serpilay; Benzer, Hilal; Toran, Meryem Kocaslan; Toygar, IsmailBackgroundFoot care self-management is underutilized in older adults and diabetic foot ulcers are more common in older adults. It is important to identify predictors of foot care self-management in older adults with diabetes in order to identify and support vulnerable groups. This study aimed to identify predictors of foot care self-management in older adults with diabetes using a machine learning approach.MethodThis cross-sectional study was conducted between November 2023 and February 2024. The data were collected in the endocrinology and metabolic diseases departments of three hospitals in Turkey. Patient identification form and the Foot Care Scale for Older Diabetics (FCS-OD) were used for data collection. Gradient boosting algorithms were used to predict the variable importance. Three machine learning algorithms were used in the study: XGBoost, LightGBM and Random Forest. The algorithms were used to predict patients with a score below or above the mean FCS-OD score.ResultsXGBoost had the best performance (AUC: 0.7469). The common predictors of the models were age (0.0534), gender (0.0038), perceived health status (0.0218), and treatment regimen (0.0027). The XGBoost model, which had the highest AUC value, also identified income level (0.0055) and A1c (0.0020) as predictors of the FCS-OD score.ConclusionThe study identified age, gender, perceived health status, treatment regimen, income level and A1c as predictors of foot care self-management in older adults with diabetes. Attention should be given to improving foot care self-management among this vulnerable group.Öğe Editorial: Understanding the mesenchymal to epithelial transition: a much needed angle for epithelial mesenchymal plasticity(Frontiers Media SA, 2024) Alotaibi, Hani; Meuwissen, Ralph; Sayan, A. EmreThe epithelial-mesenchymal transition (EMT) and the mesenchymal-epithelial transition (MET) are two critical biological processes with functions that extend beyond their role during development to wound healing and cancer metastasis. However, despite the wealth of research on EMT, MET remains underexplored. Recent studies have highlighted the importance of transcriptional and epigenetic regulation of MET, particularly in cancer biology. This review summarizes key developments in the regulation of MET at the transcriptional level, focusing on findings from recent research, including the Research Topic “Understanding the Mesenchymal to Epithelial Transition: A Much Needed Angle for Epithelial-Mesenchymal Plasticity” published in Frontiers in Cell and Developmental Biology.Öğe The impact of disgust learning on memory processes for neutral stimuli: a classical conditioning approach(Routledge journals, 2024) Soylemez, Sinem; Kapucu, AycanDisgust is a basic emotion that promotes pathogen avoidance and can contaminate nearby neutral stimuli. This study investigates how neutral stimuli, which have acquired disgust value through classical conditioning, are processed in episodic memory. The Category Conditioning paradigm was utilised to assign emotional significance to neutral stimuli, followed by a recognition test conducted immediately or 24 h after conditioning (Experiment 1). The results revealed that neutral stimuli that acquired disgust value were recognised with greater accuracy and higher liberal bias compared to other neutral stimuli in the recognition test conducted after 24 h, but not immediately. Present study also indicates that the memory enhancement observed with disgust did not manifest in the context of fear (Experiment 2). Additionally, the results varied when neutral stimuli associated with disgust were presented with disgusting stimuli in recognition test (Experiment 3). Thus, the present study demonstrates that the memory advantage of disgust extends to associated stimuli when they are presented in a list without disgusting stimuli.Öğe Mitochondrial toxicity of selected natural compounds: in vitro assessment and in silico molecular docking and dynamics simulation(Taylor & Francis Ltd, 2024) Erguc, Ali; Albayrak, Gokay; Muhammed, Muhammed Tilahun; Karakus, Fuat; Arzuk, EgePrangos uechtritzii Boiss & Hausskn stands out for its rich bioactive constituents including prantschimgin (PRA), imperatorin (IMP), suberosin (SUB), adicardin (ADI), and oxypeucedanin hydrate (OPH) in the Apiaceae family. Although these molecules contribute to several biological activities, their mitochondrial toxicity were not illuminated in depth with the appropriate in vitro and in silico models. Cell viability studies investigated the cytotoxic activities of molecules in HepG2 cells by replacing glucose with galactose due to Warburg effects. Mitochondrial toxicity (mitotoxicity) parameters such as cellular adenosine triphosphate (ATP) and mitochondrial membrane potential (MMP) levels were assessed with cytotoxic concentrations of selected molecules. Molecular docking and dynamics studies were also conducted against mitochondrial electron transport chain (ETC) complexes (I-V) with selected compounds. In vitro results showed that PRA, SUB, and IMP reduced cell viability more in galactose media compared to high glucose media in a dose-dependent manner. PRA, IMP, and SUB decreased ATP levels and MMP, especially in the galactose medium. The in silico study revealed that PRA, IMP, and SUB might bind to complexes I-V at different levels. The docking study demonstrated that PRA had the highest binding potential with the complexes, higher than the standard ligands in some cases. The molecular dynamics (MD) simulation study showed that PRA formed stable complexes with complexes II, III, and IV. In addition, PRA was anticipated to remain inside the binding site of complex II most stably during the 230 ns simulation period. Our study suggests that PRA, IMP, and SUB exhibit mitotoxicity.Öğe The preventive health management self-efficacy related to preterm labor scale in reproductive-aged women: Validation of Turkish reliability and factor structure(Wiley, 2024) Cirban Ekrem, Ebru; Dasikan, Zeynep; Unsal Atan, SenayAimThis study aims to test and adapt the Turkish psychometric properties of the preventive health management self-efficacy related to premature labor (PHMSE-PL) scale for women of reproductive age.MethodsThe study follows a methodological approach. The original scale comprises 34 items distributed across 5 subscales and is structured on a five-point Likert scale. The study sample consisted of 351 women aged 19 to 49, all without any physical or mental disabilities, irrespective of marital status or pregnancy status. Exclusion criteria encompassed women engaged in healthcare professions such as medical faculty, nursing, or midwifery, as well as those with a history of preterm birth during pregnancy.ResultsDuring the validity assessment of the scale, language, content, and construct validity were scrutinized. In the reliability phase, internal consistency and stability over time -analyses were conducted. Following confirmation of language validity, all items achieved a content validity index value exceeding 0.80. Exploratory and confirmatory factor analyses were employed to assess structural validity, revealing that the scale retained its original grouping into five sub-factors. These factors collectively accounted for 81.5% of the variance. The Cronbach's alpha coefficient for the entire scale reached 0.98, indicating high internal consistency. Test-retest analyses yielded a correlation coefficient of 0.99 between overall scale scores, demonstrating excellent consistency between the two measurement measurements.ConclusionsThe PHMSE-PL scale adapted to Turkish culture demonstrated high levels of validity and reliability.Öğe Can wheel polishers improve surface properties and color stability of monochromatic resin composites?(BMC, 2024) Turkun, Lezize Sebnem; Canevi, Cankut; Degirmenci, Alperen; Boyacioglu, HayalBackground: To overcome the color layering procedure, monochromatic resin composites have been introduced. However, little is known about their polishability, gloss and color stability. This study aimed to investigate the surface roughness, gloss, and color change of monochromatic resin composites polished with wheel systems after being immersed in coffee. Materials and methods: Omnichroma, Zenchroma, Essentia Universal, Charisma Diamond One and NeoSpectra ST were used to obtain 120-disc samples of 8 x 2 mm. Only one side of the sample was polished with Twist Dia (TWD) or Nova Twist (NOV). The samples were examined for surface roughness, gloss, and color (Delta E and Delta E-00) before and after 7 days of immersion in coffee and subsequent repolishing. The discs were examined via SEM. Surface roughness and gloss values were analyzed using ANOVA, Tukey and Pearson correlation tests. Delta E and Delta E00 values were evaluated using T tests, multivariate ANOVA, and Dunnett's post-hoc tests. Results: For TWD groups, the smoothest material was Omnichroma (p < 0.05), while for NOV groups, it was Omnichroma and Zenchroma. Omnichroma was the glossiest, while Charisma Diamond One was the least glossy. In TWD groups, Charisma Diamond One and Essentia Universal were the most discolored, while Zenchroma and Omnichroma were the least. For NOV groups, Essentia Universal and Charisma Diamond One were the most discolored, while NeoSpectra ST, Omnichroma and Zenchroma were the least. After repolishing, Charisma Diamond One did not reach the level of Delta E < 2, while the other groups showed values below. Color evaluation with the CIELab and CIEDE2000 systems revealed similar results for the TWD groups after post-staining. Conclusions: Smooth and glossy surfaces could be achieved with the wheel system regardless of the composite resin. Repolishing after discoloration ensures that the color recovery is below the acceptable limit. Color evaluations with CIELab and CIEDE2000 yielded similar results.Öğe Through the eyes of the parents: a transdiagnostic psychiatric perspective for children with differences of sexual development(Walter de Gruyter GMBH, 2024) Ozbaran, Burcu; Senturk Pilan, Birsen; Yavuz Kan, Oyku; Yulug Tas, Begum; Inal Kaleli, Ipek; Er, Eren; Ozen, Samim; Goksen, Damla; Darcan, SukranPurpose Existing literature lacks data on a subgroup exhibiting psychiatric symptoms below the DSM-5 diagnostic threshold within DSD cases. Our study aims to assess parental knowledge, attitudes toward DSD, and parental perceptions of emotional and behavioral states through a transdiagnostic perspective. Methods The study was conducted with a total of 35 parents of children with DSD. Two groups were established via k-means clustering, based on psychiatric symptomatology levels, derived from The Strength and Difficulties Questionnaire - Parent Form and The Revised Children's Anxiety and Depression Scale - Parent Form: with one group exhibiting lower reported psychiatric symptoms (LPS=27) and the other demonstrating higher psychiatric symptoms (HPS=8) by parents. Results Our study found that many parents were hesitant to disclose DSD diagnoses to their children, believing them to be too young to comprehend the information (42.9 %) and that they were unaware of the available support that could be provided by the medical team in disclosing the diagnosis (25.7 %). Our study found no differences in DSM-5 diagnoses between HPS and LPS groups (p>0.05), with ADHD being the most prevalent diagnosis (21.7 %) and a significant overrepresentation of children with a discrepancy between assigned gender at birth and gender upbringing in the HPS group compared to the LPS group (p<0.001). Conclusions Our study emphasizes the necessity of a transdiagnostic approach in psychiatry to move beyond binary conceptualizations and better understand the complexities of individuals with DSD.Öğe The effects of balance training in patients with total hip arthroplasty: A randomized controlled pilot study(Springer, 2024) Elibol, Nuray; Unver, Bayram; Karatosun, VasfiBackground: Total hip arthroplasty (THA) causes damage to hip joint mechanoceptors, which in turn leads to decrease in proprioception and increase in balance disorders. Few research has focused on balance training in patients with arthroplasty and none investigated the long-term effect of balance training using objective balance assessment methods in THA patients. The purpose of our study was to investigate the effects of balance training in patients with THA until 26 weeks postoperatively. Method: For this study 24 patients with hip osteoarthritis, who were candidates for THA were recruited. Of the patients with THA 16 completed the study protocol and the patients were randomized to 2 groups: conventional rehabilitation (CR, n = 8) or conventional rehabilitation plus balance training (CR + BT, n = 8). The CR group completed typical surgery-specific exercise programs, while the CR + BT group completed the CR plus balance exercises. The patients were evaluated by a hand-held dynamometer, single leg stance test (SLST), Tetrax balance system, Harris hip score, lower extremity function scale, 5 times sit to stand test and 50-foot timed walk test preoperatively and 8, 14 and 26 weeks postoperatively. ResultsWhile the CR + BT group showed significant improvement for the right extremity eyes closed SLST score (p < 0.05), there was no significant difference for other assessment parameters between the CR and CR + BT groups (p > 0.05). There were significant improvements in both groups until 26 weeks following THA (p < 0.05). ConclusionThe results of our study indicate that there were similar improvements in the balance and functional parameters in the CR and CR + BT groups. There was no additional benefit after 26 weeks of controlled balance exercises following THA.Öğe Prognostic impact of next-generation sequencing on myelodysplastic syndrome: A single-center experience(Lippincott Williams & Wilkins, 2024) Bulbul, Hale; Kaya, Ozge Ozer; Karadag, Fatma Keklik; Olgun, Aybuke; Demirci, Zuhal; Ceylan, CengizMyelodysplastic syndromes (MDS) are clinically heterogeneous disorders characterized by peripheral blood cytopenias, poor differentiation, clonal hematopoiesis, and increased risk of developing acute myeloid leukemia (AML). While somatic mutations do not currently feature in prognostic scoring systems, they may impact the clinical phenotype. In recent years, next-generation sequencing (NGS) has enabled the opportunity to identify an increasing number of genetic abnormalities, including recurrent modifications in the TP53, DNMT3A, NRAS, NPM1, RUNX1, and FLT3 genes. Bone marrow aspirate samples of 56 patients with MDS were investigated for mutations using NGS. We compared the relationship between gene mutation status and laboratory characteristics, such as certain cytopenias, the revised international prognostic scoring system, MDS subtypes, karyotypes, AML development, and overall survival. Twenty-one genes were found to have gene mutations, including ASXL1, TET2, SRSF2, EZH2, CSF3R, NRAS, ETV6, SETBP1, RUNX1, DDX41, U2AF1, JAK2, FLT3ITD, SF3B1, DNAMT3A, PHF6, TP53, CEBPA, CBL, IDH2, and GATA2. At least one point mutation occurred in 64.2% of all patients, including 58.3% of those with normal cytogenetics. Thrombocytopenia (P = .016), anemia (P = .018), decreased overall survival (P = .017), and increased AML transformation (P = .023) have been revealed to be linked to non-SF3B1 mutations. MDS are frequently associated with somatic point mutations. According to early findings, NGS panels are extremely effective instruments that provide an entirely new viewpoint on the disease for particular individuals. Future prognostications will depend more on NGS because those who exhibit normal cytogenetics may additionally have gene mutations.Öğe Preparation of dual drug-loaded polymer nanoconjugate to enhance treatment efficacy for ovarian cancer cells(Elsevier, 2024) Ozel, Buket; Sanlier, Senay; Gunduz, Cumhur; Gunel, Nur SelviOvarian cancer is the deadliest gynecological malignancy, representing 2.5 % of all female cancers and accounting for 5 % of female cancer-related fatalities. Despite numerous strategies in its treatment, the disease shows a high recurrence rate and a low survival rate. Consequently, there is a growing focus on targeted therapies in ovarian cancer treatment. It is well-known that VEGFR and LPA pathways undergo alterations in ovarian cancer and stimulate survival, adhesion, migration, invasion, tumor growth and angiogenesis. Cabozantinib (CBZ) is a multi-receptor tyrosine kinase inhibitor that effectively targets MET, VEGFR-1, 2, 3, FLT3, c-KIT, and RET. Ki16425 is a selective inhibitor of LPA receptors 1, 2, and 3. Therefore, targeting LPA receptors and combining with VEGFR inhibitor is a strategic approach for ovarian cancer treatment. In this study, it was aimed to prepare polymer-drug nanoconjugate for both VEGFR and LPAR inhibition. For this, O-(2-Carboxyethyl) polyethylene glycol (PEG5000) 5000 ) which advantages are known in cancer studies, was chosen as the carrier system, and a nanoconjugate containing Ki16425 and CBZ (Ki-PEG-CBZ) was synthesized and its potential was evaluated. Initially, CBZ and Ki16425 were conjugated to the PEG 5000 through pH-sensitive hydrazone and ester bonds. After nanoconjugate characterization, in vitro release and its ovarian cancer treatment potential were evaluated on A2780, OVCAR3 and SKOV3 ovarian cancer cell lines. A nanoconjugate was obtained with a particle size of 169 f 15.23 nm, a zeta potential of-13.5 f 1.21 mV, and a release profile lasting 48 h, containing CBZ and Ki16425 with drug loading efficiencies of 73.71 f 0.53% and 77.72 f 2.51 %, respectively. In vitro studies have demonstrated that Ki-PEG-CBZ is highly effective against ovarian cancer. We suggest that the developed polymer-drug nanoconjugate is an effective and safe nanoconjugate for the treatment of ovarian cancer.Öğe Optimized aptamer-based next generation biosensor for the ultra-sensitive determination of SARS-CoV-2 S1 protein in saliva samples(Wiley, 2024) Erdem, Arzum; Senturk, Huseyin; Yildiz, Esma; Maral, MeltemCOVID-19 is an infectious disease caused by the SARS-CoV-2 virus, which rapidly spread worldwide and resulted in a pandemic. Efficient and sensitive detection techniques have been devised since the onset of the epidemic and continue to be improved at present. Due to the crucial role of the SARS-CoV-2 S1 protein in facilitating the virus's entry into cells, efforts in detection and treatment have primarily centered upon this protein. In this study, a rapid, ultrasensitive, disposable, easy-to-use, cost-effective next generation biosensor based on optimized aptamer (Optimer, OPT) was developed by using a disposable pencil graphite electrode (PGE) and applied for the impedimetric determination of SARS-CoV-2 S1 protein. The S1 protein interacted with the OPT in the solution phase and then immobilized onto the PGE surface. Subsequently, measurements using electrochemical impedance spectroscopy (EIS) were conducted in a solution containing a redox probe of 1 mM [Fe(CN)6]3- /4- . Under optimum conditions, the limit of detection (LOD) for the S1 protein in buffer medium at concentrations ranging from 101 to 106 ag/mL was calculated as 8.80 ag/mL (0.11 aM). The selectivity of the developed biosensor was studied against MERS-CoV-S1 protein (MERS) and Influenza Hemagglutinin antigen (HA). Furthermore, the application of the biosensor in artificial saliva medium is demonstrated. The LOD was also calculated in artificial saliva medium in the concentration range of 101-105 ag/mL and calculated as 2.01 ag/mL (0.025 aM). This medium was also used to assess the selectivity of optimized-aptamer based biosensor.Öğe Exploring the efficacy and tolerability of two-injection start regimen of long-acting aripiprazole: A descriptive case series analysis(Wiley, 2024) Sungur, Ibrahim; Keskin, Kaan; Aktas, Elif Ozge; Eker, Mehmet CagdasBipolar disorder is the fourth most debilitating psychiatric illness in the world regarding Disability Adjusted Life Years and manic episodes frequently lead to lengthy hospitalizations which restricts the freedom of patients. Therefore, decreasing the length of hospitalization with safer agents is of utmost importance in the treatment of manic episodes. Aripiprazole is a medication known for its efficacy in managing mania associated with bipolar disorder. Aripiprazole long-acting injection is approved for the treatment of mania associated with bipolar disorder in adults and found efficacious as a maintenance treatment. In the treatment of schizophrenia, European Medicines Agency has approved a simplified starting strategy of aripiprazole once a month, with two 400 mg injections and a single oral 20 mg dose of aripiprazole. To the best of our knowledge, no previous study has reported the safety, tolerability, and efficacy of this regimen in adult bipolar disorder patients. We present a case series of eight patients who were admitted to the hospital in a manic episode with psychotic features. We observed that the double injection start regimen was effective in treating manic symptoms with no specific severe adverse events. We conclude from a small sample of manic patients that a double injection start regimen has good efficacy and tolerability. Evaluate the safety, tolerability, and efficacy of the TIS regimen of Aripiprazole in treating manic episodes in bipolar disorder. Retrospective observational study involving eight hospitalized patients with bipolar disorder in a manic episode.imageÖğe Turkish translation and validation of an interprofessional identity measure: EPIS-TR(Taylor & Francis Inc, 2024) Kolcu, Giray; Baser Kolcu, Mukadder Inci; Krijnen, Wim; Reinders, Jan-JaapResearch on interprofessional identity using the Extended Professional Identity Theory (EPIT) has shown promising results in measuring this identity, predicting interprofessional collaboration, and improving team outcomes. However, EPIT-based interprofessional identity has not been studied in T & uuml;rkiye due to the absence of a Turkish version of the Extended Professional Identity Scale (EPIS). We aimed to develop and validate a Turkish EPIS, the EPIS-TR, and explore interprofessional identities across eight different professions. A cross-cultural adaptation of EPIS was made by linguistic validation, followed by analysis of psychometric properties using a sample of students from four Turkish universities and eight professions/programs (n = 405). Confirmatory factor analysis and reliability analyses were performed. Differences between professions were explored with ANOVA and a post hoc test. Three out of seven goodness-of-fit indices showed acceptable fit (x2/df = .004; RMSEA = .07; GFI = .94), and others showed excellent fit (SRMR = .04; AGFI = .91; CFI = .97; NNFI = .96). The internal consistency of the EPIS-TR is .93. The EPIS-TR scale has strong psychometric properties. The construct validity of the EPIS-TR was similar to that of the original version. The interprofessional identity scores of the different professions were mostly similar. Based on these findings, the EPIS-TR is well suited for measuring interprofessional identity.Öğe Outcomes of HeartMate 3 in pediatric patients with end-stage heart failure: a single-center preliminary experience from Turkey(Frontiers Media SA, 2024) Dogan, Eser; Ulger Tutar, Zulal; Tuncer, Osman Nuri; Levent, Resit E.; Engin, Cagatay; Yagdi, Tahir; Atay, Yuksel; Ozbaran, MustafaObjectives We aim to evaluate our initial experience with the HeartMate 3 (HM3) device (Abbott, USA) for palliating pediatric patients with end-stage heart failure (ESHF).Methods We conducted a retrospective review of clinical data from pediatric patients (aged 7-18 years) who underwent HM3 implantation for ESHF at our institution between 2022 and 2024. Patient demographics and follow-up data were comprehensively analyzed.Results We identified 11 patients (45% males) with a median age of 14 years (IQR 11-17), a median weight of 47 kg (IQR 28-50), a median height of 159 cm (IQR 135-165), and a median body surface area of 1.36 m2 (IQR 1.07-1.53) at the time of the intervention. All patients were diagnosed with dilated cardiomyopathy and categorized with PEDIMACS profiles ranging from one to three. The median ICU stay was 14 days (IQR 6-32), with 11 patients receiving inotropic support for a median of four postoperative days (IQR 3-8). The median follow-up period was 150 days (IQR 90-210). Early complications included two cases of pleural effusion, 1 case of cardiac tamponade, 3 cases of polyuria, and one instance of positive blood cultures. One patient, who was non-compliant with warfarin therapy, developed a thrombus in the right atrium that was resolved with a revision of anticoagulant therapy, and did not experience pump thrombosis. During follow-up, one patient died after 28 days from sepsis, one underwent heart transplantation after 10 days, and nine patients remained alive on the device. Notably, there were no reported cases of pump thrombosis, ischemia, or stroke post- implantation.Conclusions The HM3 device appears to be a safe and effective palliative option for pediatric patients with ESHF.
