Preparation of glutathione loaded nanoemulsions and testing of hepatoprotective activity on THLE-2 cells

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dc.authoridGeyik, Caner/0000-0002-8382-2186
dc.authoridTimur, Suna/0000-0002-1981-7577
dc.authoridYesiltepe, Ozan/0000-0003-1914-1201
dc.authorscopusid57190496212
dc.authorscopusid57201134997
dc.authorscopusid36175828200
dc.authorscopusid56322932500
dc.authorscopusid57219316379
dc.authorscopusid7801443298
dc.authorscopusid6701638388
dc.authorwosidGeyik, Caner/I-8384-2014
dc.authorwosidTimur, Suna/CAG-0914-2022
dc.contributor.authorYesiltepe, Ozan
dc.contributor.authorCelik, Emine Guler
dc.contributor.authorGeyik, Caner
dc.contributor.authorGumus, Zinar Pinar
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorCoskunol, Hakan
dc.contributor.authorTimur, Suna
dc.date.accessioned2023-01-12T20:16:03Z
dc.date.available2023-01-12T20:16:03Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractTo improve bioavailability and stability of hydrophobic and hydrophilic compounds, nanoemulsions are good alternatives as delivery systems because of their nontoxic and nonirritant nature. Glutathione (GSH) suffers from low stability in water, where its encapsulation in nanoemulsions is a powerful strategy to its stability in aqueous systems. The aim of this study was to obtain nanoemulsions from the hydrophobic/hydrophilic contents of N. sativa seed oil so as to improve GSH stability along with bioavailability of N. sativa seed oil. Then, the prepared nanoemulsions were tested for in vitro hepatoprotective activity against ethanol toxicity. To the best of our knowledge, there is no study on the test of nanoemulsions by the combination of Nigella sativa seed oils and GSH in hepatoprotective activity. Here, nanoemulsions with different contents were prepared using Nigella sativa seed oils. Content analyses and characterisation studies of prepared nanoemulsions were carried out. In order to investigate the protective effects against to ethanol exposure, THLE-2 cells were pretreated with nanoemulsions for 2 h with the maximum benign dose (0.5 mg/mL of nanoemulsions). Ethanol (400 mM) was introduced to pretreated cells and nontreated cells for 48- or 72-h periods, followed by cell viability assay was carried out. Fluorescence microscopy tests revealed the introduction of the nanoemulsions into THLE-2 cells. The findings show that nanoformulations have promising in vitro hepatoprotective effects on the THLE-2 cell line against ethanol exposure.en_US
dc.identifier.doi10.3906/kim-2007-54
dc.identifier.endpage451en_US
dc.identifier.issn1300-0527
dc.identifier.issue2en_US
dc.identifier.pmid34104055en_US
dc.identifier.scopus2-s2.0-85106285342en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage436en_US
dc.identifier.trdizinid525477en_US
dc.identifier.urihttps://doi.org/10.3906/kim-2007-54
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/525477
dc.identifier.urihttps://hdl.handle.net/11454/78635
dc.identifier.volume45en_US
dc.identifier.wosWOS:000644696500016en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherScientific Technical Research Council Turkey-Tubitaken_US
dc.relation.ispartofTurkish Journal of Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEthanolen_US
dc.subjectNigella sativaen_US
dc.subjectglutathioneen_US
dc.subjectnanoemulsionen_US
dc.subjecthepatoprotective activityen_US
dc.subjectNigella-Sativaen_US
dc.subjectIn-Vitroen_US
dc.subjectAntioxidant Propertiesen_US
dc.subjectColorimetric Assayen_US
dc.subjectGold Nanoparticlesen_US
dc.subjectLiver-Injuryen_US
dc.subjectEthanolen_US
dc.subjectSeeden_US
dc.subjectOilen_US
dc.subjectMetabolismen_US
dc.titlePreparation of glutathione loaded nanoemulsions and testing of hepatoprotective activity on THLE-2 cellsen_US
dc.typeArticleen_US

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