Bacterial production of ciprofloxacin and potential usage as a radiotracer

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dc.authorscopusid57216968604
dc.authorscopusid55911862900
dc.authorscopusid58621752700
dc.authorscopusid57192664561
dc.authorscopusid55668684600
dc.contributor.authorKaratay, K.B.
dc.contributor.authorGungor, N.D.
dc.contributor.authorColak, B.
dc.contributor.authorMuftuler, F.Z.B.
dc.contributor.authorAras, O.
dc.date.accessioned2024-08-25T18:48:03Z
dc.date.available2024-08-25T18:48:03Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractInfectious diseases caused by bacteria that have become resistant to antibiotics have increased in prevalence, necessitating new methods for their diagnosis and treatment. The aim of this study was to compare the efficacy of synthetic ciprofloxacin to that of organic ciprofloxacin produced by cave microorganisms, as well as to evaluate the feasibility of using organic ciprofloxacin radiolabeled with technetium-99m as an imaging agent. Organic ciprofloxacin produced by cave bacteria isolated from sediment taken from the dark zone of Antalya's "Yark Sinkhole,"(Turkey's 14th deepest cave), was purified using high-performance liquid chromatography. Purified organic ciprofloxacin and standard ciprofloxacin were radiolabeled with technetium-99m (99mTc), and their uptake by pathogenic microorganisms as well as potential as an imaging agent were examined. According to thin-layer radiochromatography, radiolabeling efficiencies were 98.99 ± 0.34 (n = 7) and 91.25 ± 1.84 (n = 7) for radiolabeled organic ciprofloxacin and standard ciprofloxacin respectively. The binding efficiency of radiolabeled organic ciprofloxacin at the 240th minute was higher compared with radiolabeled standard ciprofloxacin, especially with P.aeruginosa, MRSA, VRE and E.coli. The results demonstrate that radiolabeling with 99mTc does not alter the biological behavior of organic ciprofloxacin, and radiolabeled organic ciprofloxacin has potential as an imaging agent for the detection of bacterial infection. The original value of the study is the monitoring of the antibiofilm effects of untouched cave-derived organic antibiotics by radiolabeling with a radionuclide. © 2023 Karatay et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.identifier.doi10.1371/journal.pone.0291342
dc.identifier.issn1932-6203
dc.identifier.issue11 Novemberen_US
dc.identifier.pmid37943851en_US
dc.identifier.scopus2-s2.0-85176445764en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0291342
dc.identifier.urihttps://hdl.handle.net/11454/102121
dc.identifier.volume18en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240825_Gen_US
dc.subjectantibiotic agenten_US
dc.subjectbacterial DNAen_US
dc.subjectciprofloxacinen_US
dc.subjectradioisotopeen_US
dc.subjecttechnetium 99men_US
dc.subjecttraceren_US
dc.subjectantiinfective agenten_US
dc.subjectciprofloxacinen_US
dc.subjectradiopharmaceutical agenten_US
dc.subjecttechnetiumen_US
dc.subjectantibiofilm activityen_US
dc.subjectantibiotic resistanceen_US
dc.subjectantimicrobial activityen_US
dc.subjectArticleen_US
dc.subjectbacterial infectionen_US
dc.subjectbacterial strainen_US
dc.subjectbacteriumen_US
dc.subjectbacterium isolationen_US
dc.subjectCandida albicansen_US
dc.subjectcaveen_US
dc.subjectcolony forming uniten_US
dc.subjectDNA extractionen_US
dc.subjectdrug efficacyen_US
dc.subjectelectrosprayen_US
dc.subjectEscherichia colien_US
dc.subjecthigh performance liquid chromatographyen_US
dc.subjectlinear regression analysisen_US
dc.subjectliquid chromatography-mass spectrometryen_US
dc.subjectmethicillin resistant Staphylococcus aureusen_US
dc.subjectMicrococcus luteusen_US
dc.subjectminimum inhibitory concentrationen_US
dc.subjectnonhumanen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectprevalenceen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectquality controlen_US
dc.subjectradiochromatographyen_US
dc.subjectradiolabelingen_US
dc.subjectsedimenten_US
dc.subjectStaphylococcus aureusen_US
dc.subjectthin layer chromatographyen_US
dc.subjectvancomycin resistant Enterococcusen_US
dc.subjectchemistryen_US
dc.subjectmetabolismen_US
dc.subjectscintiscanningen_US
dc.subjectAnti-Bacterial Agentsen_US
dc.subjectCiprofloxacinen_US
dc.subjectEscherichia colien_US
dc.subjectRadionuclide Imagingen_US
dc.subjectRadiopharmaceuticalsen_US
dc.subjectTechnetiumen_US
dc.titleBacterial production of ciprofloxacin and potential usage as a radiotraceren_US
dc.typeArticleen_US

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