Bacterial production of ciprofloxacin and potential usage as a radiotracer
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Public Library of Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Infectious diseases caused by bacteria that have become resistant to antibiotics have increased in prevalence, necessitating new methods for their diagnosis and treatment. The aim of this study was to compare the efficacy of synthetic ciprofloxacin to that of organic ciprofloxacin produced by cave microorganisms, as well as to evaluate the feasibility of using organic ciprofloxacin radiolabeled with technetium-99m as an imaging agent. Organic ciprofloxacin produced by cave bacteria isolated from sediment taken from the dark zone of Antalya's "Yark Sinkhole,"(Turkey's 14th deepest cave), was purified using high-performance liquid chromatography. Purified organic ciprofloxacin and standard ciprofloxacin were radiolabeled with technetium-99m (99mTc), and their uptake by pathogenic microorganisms as well as potential as an imaging agent were examined. According to thin-layer radiochromatography, radiolabeling efficiencies were 98.99 ± 0.34 (n = 7) and 91.25 ± 1.84 (n = 7) for radiolabeled organic ciprofloxacin and standard ciprofloxacin respectively. The binding efficiency of radiolabeled organic ciprofloxacin at the 240th minute was higher compared with radiolabeled standard ciprofloxacin, especially with P.aeruginosa, MRSA, VRE and E.coli. The results demonstrate that radiolabeling with 99mTc does not alter the biological behavior of organic ciprofloxacin, and radiolabeled organic ciprofloxacin has potential as an imaging agent for the detection of bacterial infection. The original value of the study is the monitoring of the antibiofilm effects of untouched cave-derived organic antibiotics by radiolabeling with a radionuclide. © 2023 Karatay et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Açıklama
Anahtar Kelimeler
antibiotic agent, bacterial DNA, ciprofloxacin, radioisotope, technetium 99m, tracer, antiinfective agent, ciprofloxacin, radiopharmaceutical agent, technetium, antibiofilm activity, antibiotic resistance, antimicrobial activity, Article, bacterial infection, bacterial strain, bacterium, bacterium isolation, Candida albicans, cave, colony forming unit, DNA extraction, drug efficacy, electrospray, Escherichia coli, high performance liquid chromatography, linear regression analysis, liquid chromatography-mass spectrometry, methicillin resistant Staphylococcus aureus, Micrococcus luteus, minimum inhibitory concentration, nonhuman, polymerase chain reaction, prevalence, Pseudomonas aeruginosa, quality control, radiochromatography, radiolabeling, sediment, Staphylococcus aureus, thin layer chromatography, vancomycin resistant Enterococcus, chemistry, metabolism, scintiscanning, Anti-Bacterial Agents, Ciprofloxacin, Escherichia coli, Radionuclide Imaging, Radiopharmaceuticals, Technetium
Kaynak
PLoS ONE
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
18
Sayı
11 November
