The effects of mesenchymal stem cells on lymphoblastic leukemia cell proliferation

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dc.contributor.authorCetintas, Vildan Bozok
dc.contributor.authorAktug, Huseyin
dc.contributor.authorOltulu, Fatih
dc.contributor.authorKeskinoglu, Ahmet
dc.contributor.authorDel Castello, Buket Erer
dc.contributor.authorTaskiran, Dilek
dc.date.accessioned2019-10-27T22:13:01Z
dc.date.available2019-10-27T22:13:01Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractPurpose: Mesenchymal stein cells (MSCs) represent a new approach to the treatment of several neoplastic or non-neoplastic disorders. Their potential to repair damaged tissues through trans differentiation in conjunction with their immunomodulatory ability made them promising candidates for cell-based immunotherapy and regenerative medicine. In the present study, we aimed to determine the effects of MSCs on proliferation, apoptosis and gene expression profile of the acute lymphoblastic leukemia (ALL) cell line CCRF-CEM. Methods: The experiments were performed after MSCs and CCRF-CEM cells were co-cultured for 72hrs. We analyzed the gene expression patterns to predict oncogenic pathway dysregulation in the cell groups by quantitative RT-PCR and immunohistochemical staining. Results: Cell proliferation was significantly inhibited in co-cultured CCRF-CE.M cells compared to the control. Furthermore, growth factors, p53, Box and Caspase-9 expressions were increased and cell-signaling gene expressions decreased significantly. Despite increased levels of growth factors (CTGF, VEGF, FGF, EGFR), the increased apoptosis level was triggered by p53/ Bax. Conclusion: In this study we have shown that human MSCs have inhibitory effect on their neighboring malignant leukemia cells.en_US
dc.description.sponsorshipEge University Research FundEge University [2010-TIP-070]en_US
dc.description.sponsorshipThis work was supported by the Ege University Research Fund (2010-TIP-070).en_US
dc.identifier.endpage1017en_US
dc.identifier.issn1107-0625
dc.identifier.issn2241-6293
dc.identifier.issue4en_US
dc.identifier.pmid25536609en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1006en_US
dc.identifier.urihttps://hdl.handle.net/11454/49670
dc.identifier.volume19en_US
dc.identifier.wosWOS:000347742000023en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherImprimatur Publicationsen_US
dc.relation.ispartofJournal of Buonen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectacute lymphoblastic leukemiaen_US
dc.subjectapoptosisen_US
dc.subjectCCRF-CEM cell lineen_US
dc.subjectco-cultureen_US
dc.subjectmesenchymal stem cellsen_US
dc.titleThe effects of mesenchymal stem cells on lymphoblastic leukemia cell proliferationen_US
dc.typeArticleen_US

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