The effects of mesenchymal stem cells on lymphoblastic leukemia cell proliferation
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Imprimatur Publications
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose: Mesenchymal stein cells (MSCs) represent a new approach to the treatment of several neoplastic or non-neoplastic disorders. Their potential to repair damaged tissues through trans differentiation in conjunction with their immunomodulatory ability made them promising candidates for cell-based immunotherapy and regenerative medicine. In the present study, we aimed to determine the effects of MSCs on proliferation, apoptosis and gene expression profile of the acute lymphoblastic leukemia (ALL) cell line CCRF-CEM. Methods: The experiments were performed after MSCs and CCRF-CEM cells were co-cultured for 72hrs. We analyzed the gene expression patterns to predict oncogenic pathway dysregulation in the cell groups by quantitative RT-PCR and immunohistochemical staining. Results: Cell proliferation was significantly inhibited in co-cultured CCRF-CE.M cells compared to the control. Furthermore, growth factors, p53, Box and Caspase-9 expressions were increased and cell-signaling gene expressions decreased significantly. Despite increased levels of growth factors (CTGF, VEGF, FGF, EGFR), the increased apoptosis level was triggered by p53/ Bax. Conclusion: In this study we have shown that human MSCs have inhibitory effect on their neighboring malignant leukemia cells.
Açıklama
Anahtar Kelimeler
acute lymphoblastic leukemia, apoptosis, CCRF-CEM cell line, co-culture, mesenchymal stem cells
Kaynak
Journal of Buon
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
19
Sayı
4
