A new approach for in vitro imaging of breast cancer cells by anti-metadherin targeted PVA-pyrene
Küçük Resim Yok
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-VCH Verlag
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Poly(vinyl alcohol)-pyrene-anti-metadherin (PVA-Py-(Anti-MTDH)), a novel antibody based water soluble probe containing both fluorescent and target sites in the structure for in vitro imaging of breast cancer cells is reported here. Since breast cancer cells have an excess of MDTH protein expressed on the surface, a PVA-Py prepared by "Click chemistry" approach is targeted by Anti-MTDH antibody and applied to the MCF-7 cell line. After characterization, the designed architecture was evaluated in terms of cell incorporation efficiency and compared with a non-targeted structure (PVA-Py). Atomic force microscopy (AFM) and fluorescence microscopy images of cells after incubation of the probe molecules were also obtained to monitor the interaction of the probes with the cancerous cells. (Figure Presented). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Açıklama
Anahtar Kelimeler
Drug delivery systems, Fluorescence, Functionalization of polymers, Imaging, Synthesis
Kaynak
Macromolecular Bioscience
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
10
Sayı
6
