Autophagy Markers Induced by Influenza Virus and MUC1 Expressions in Cancer-Derived Cell Lines

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dc.authorscopusid57549113900
dc.authorscopusid57414918400
dc.authorscopusid57250647600
dc.authorscopusid57208211340
dc.authorscopusid35611300300
dc.contributor.authorÖzkan, H.S.
dc.contributor.authorÖzarslan, A.
dc.contributor.authorVatansever, G.
dc.contributor.authorDurmaz, S.
dc.contributor.authorÇiçek, C.
dc.date.accessioned2024-08-25T18:53:39Z
dc.date.available2024-08-25T18:53:39Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractInfluenza virus-induced autophagy is often accompanied by apoptosis and results in cell death in virus-infected cells. It is well known that autophagy is modulated by the mTOR/PI3K/Akt pathway, which plays an important role in the response to the presence of energy sources and external stimuli. This pathway is modulated by mucin 1 (MUC1), which has extracellular and intracellular components and plays an important role in metastasis and chemotherapeutic resistance. In this study, it was aimed to investigate the expression of MUC1 after the inoculation of influenza viruses into the cancer-derived cell cultures and, accordingly, the changes in autophagy markers such as mTOR and LC3B. In this study, MCF-7, HeLa and A-549 cell lines were used which have adenocarcinoma origin. To control the growth of influenza virus in these cells, the MDCK cell line was also inoculated. Centrifuge-enhanced shell-vial cell culture method was used in all experiments. Influenza A (H1N1) pdm09 strain was inoculated into these cell lines then the expressions of viral nucleic acid and cycle threshold (Ct) of MUC1, mTOR, LC3B associated genes were investigated by quantitative real-time reverse transcriptase polymerase chain reaction (qRTPCR) method in the samples taken from the supernatants of all cells at the end of the 48-hour incubation period. To investigate whether these markers were present in cells, after all cells were permeabilized with paraformaldehyde, cell-coated infected coverslips were stained with fluorescent labeled monoclonal antibodies developed against MUC1, mTOR, LC3B and influenza virus antigens. In the examination of fluorescence microscopy, all of the cell cultures (MCF-7, He-La and A-549) infected with influenza virus yielded positive results in terms of LC3B, mTOR and MUC1 monoclonal antibody staining, whereas all of the non-infected cells were found negative. Cycle threshold values of MUC1, LC3B and mTOR associated genes were found to be lower in A-549 cell line inoculated with influenza virus. Although protein expression was demonstrated in MCF-7 and He-La cell lines, similar changes were not detected in the 1/Ct values of genes in the autophagy pathway. The Ct value of the MUC1 gene was found to be higher only in the MCF-7 cell line after inoculation. In conclusion, it was observed that the specific expression pattern for influenza virus-induced autophagy was formed only in the A-549 cell line among the adenocarcinoma cells. It was thought that this relationship could constitute a dataset in further research on lung adenocarcinoma. However, in future studies, the determination of the expression of these genes at the protein level by using further tests will provide better comparison of the results.en_US
dc.identifier.doi10.5578/mb.20239932
dc.identifier.endpage400en_US
dc.identifier.issn0374-9096
dc.identifier.issue3en_US
dc.identifier.pmid37462303en_US
dc.identifier.scopus2-s2.0-85164997648en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage390en_US
dc.identifier.urihttps://doi.org/10.5578/mb.20239932
dc.identifier.urihttps://hdl.handle.net/11454/103195
dc.identifier.volume57en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherNLM (Medline)en_US
dc.relation.ispartofMikrobiyoloji bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240825_Gen_US
dc.subjectMUC1 protein, humanen_US
dc.subjectmucin 1en_US
dc.subjectphosphatidylinositol 3 kinaseen_US
dc.subjecttarget of rapamycin kinaseen_US
dc.subjectadenocarcinomaen_US
dc.subjectcell lineen_US
dc.subjectgeneticsen_US
dc.subjecthumanen_US
dc.subjectInfluenza A virus (H1N1)en_US
dc.subjectmetabolismen_US
dc.subjectAdenocarcinomaen_US
dc.subjectAutophagyen_US
dc.subjectCell Lineen_US
dc.subjectHumansen_US
dc.subjectInfluenza A Virus, H1N1 Subtypeen_US
dc.subjectMucin-1en_US
dc.subjectPhosphatidylinositol 3-Kinasesen_US
dc.subjectTOR Serine-Threonine Kinasesen_US
dc.titleAutophagy Markers Induced by Influenza Virus and MUC1 Expressions in Cancer-Derived Cell Linesen_US
dc.title.alternativeKanser Kökenli Hücre Dizilerinde İnfluenza Virüsünün İndüklediği Otofaji Belirteçleri ile MUC1 Ekspresyonu Arasındaki İlişkinin Araştırılmasıen_US
dc.typeArticleen_US

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