In vitro T lymphocyte proliferation by carboxyfluorescein diacetate succinimidyl ester method is helpful in diagnosing and managing primary immunodeficiencies

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dc.contributor.authorAzarsiz, Elif
dc.contributor.authorKaraca, Neslihan
dc.contributor.authorErgun, Birgul
dc.contributor.authorDurmuscan, Mehmet
dc.contributor.authorKutukculer, Necil
dc.contributor.authorAksu, Guzide
dc.date.accessioned2019-10-27T10:46:46Z
dc.date.available2019-10-27T10:46:46Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractBackgroundFunctional studies besides routine laboratory tests for the definitive diagnosis of T lymphocyte disorders with isolated T or combined T/B-cell immunodeficiencies are important. We hereby summarized our experience with a carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay for the assessment of mitogenic T-cell proliferation responses in primary immunodeficiency (PID) patients who have not been diagnosed yet or genetically analyzed, but classified as probably having T-cell defects. MethodsUnclassified patients (n=46) and controls (n=25) were evaluated for T-cell disorders with CFSE-based assay. ResultsCD3(+) blast cells after PHA-L stimulation were significantly lower in patients (31.128.8) than controls (67.9 +/- 8.79; P<.001). Nine patients with low and four patients with normal CD3 values had severely decreased blastic transformation. The proliferation response decreased mostly in combined immunodeficiency group. Sixteen of them had impaired proliferation responses. Appropriate molecular genetical analyses were planned after thorough evaluation of each patient. ConclusionsIn vitro lymphocyte cell proliferation analysis by CFSE method is a reliable and practical choice for the assessment of mitogenic T lymphocyte responses in yet unclassified PID patients for targeting further genetical analyses.en_US
dc.identifier.doi10.1002/jcla.22216en_US
dc.identifier.issn0887-8013
dc.identifier.issn1098-2825
dc.identifier.issue1en_US
dc.identifier.pmid28383134en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1002/jcla.22216
dc.identifier.urihttps://hdl.handle.net/11454/31343
dc.identifier.volume32en_US
dc.identifier.wosWOS:000423046100063en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Clinical Laboratory Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcarboxyfluorescein diacetate succinimidyl esteren_US
dc.subjectimmunodeficiencyen_US
dc.subjectT lymphocyte proliferationen_US
dc.titleIn vitro T lymphocyte proliferation by carboxyfluorescein diacetate succinimidyl ester method is helpful in diagnosing and managing primary immunodeficienciesen_US
dc.typeArticleen_US

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