In vitro T lymphocyte proliferation by carboxyfluorescein diacetate succinimidyl ester method is helpful in diagnosing and managing primary immunodeficiencies
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
BackgroundFunctional studies besides routine laboratory tests for the definitive diagnosis of T lymphocyte disorders with isolated T or combined T/B-cell immunodeficiencies are important. We hereby summarized our experience with a carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay for the assessment of mitogenic T-cell proliferation responses in primary immunodeficiency (PID) patients who have not been diagnosed yet or genetically analyzed, but classified as probably having T-cell defects. MethodsUnclassified patients (n=46) and controls (n=25) were evaluated for T-cell disorders with CFSE-based assay. ResultsCD3(+) blast cells after PHA-L stimulation were significantly lower in patients (31.128.8) than controls (67.9 +/- 8.79; P<.001). Nine patients with low and four patients with normal CD3 values had severely decreased blastic transformation. The proliferation response decreased mostly in combined immunodeficiency group. Sixteen of them had impaired proliferation responses. Appropriate molecular genetical analyses were planned after thorough evaluation of each patient. ConclusionsIn vitro lymphocyte cell proliferation analysis by CFSE method is a reliable and practical choice for the assessment of mitogenic T lymphocyte responses in yet unclassified PID patients for targeting further genetical analyses.
Açıklama
Anahtar Kelimeler
carboxyfluorescein diacetate succinimidyl ester, immunodeficiency, T lymphocyte proliferation
Kaynak
Journal of Clinical Laboratory Analysis
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
32
Sayı
1
