DNA insitu hybridization in the diagnosis of human papillomavirus infection

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dc.contributor.authorErensoy, S
dc.contributor.authorErhan, Y
dc.contributor.authorZeytinoglu, A
dc.contributor.authorOzacar, T
dc.contributor.authorOzdemir, N
dc.contributor.authorBilgic, A
dc.date.accessioned2019-10-27T11:55:40Z
dc.date.available2019-10-27T11:55:40Z
dc.date.issued1996
dc.departmentEge Üniversitesien_US
dc.descriptionInternational Symposium on Recent Advances in the Diagnosis of Viral Diseases -- JUL 20-22, 1995 -- ISTANBUL, TURKEYen_US
dc.description.abstractBackground: Certain types of human papillomavirus (HPV) are shown to be associated with the development of genital lesions. DNA hybridization methods are used for the diagnosis of HPV infections. Objective: To use a nonradioactive DNA in situ hybridization system for the investigation of HPV infections responsible for the development of genital lesions in women. Study design: Sections from archival paraffin embedded biopsy specimens of 59 cases were screened for the presence of HPV DNA sequences by using digoxigenin labeled DNA probe which is specific for all types of HPVs and digoxigenin detection system. The study group consisted of samples diagnosed as squamous hyperplasia of the vulva (group 1), koilocytosis (group 2), condyloma acuminatum/koilocytotic atypy (group 3), cervical intraepithelial neoplasia (CIN), and epidermoid carcinoma (group 4). Results: No HPV DNA was detected in groups 1 and 2 which consisted of 3 and 13 specimens respectively. Seven of 11 (63.6%) specimens in group 3 and 7 of 32 (21.9%) in group 4 were found to be positive for in situ HPV DNA. Seven positive samples in group 3 and one positive sample in group 4 were typed as HPV 6/11. Five samples of the remaining positives in group 4 were typed as HPV 16/18. One case was found to be positive with both 16/18 and 31/33. Conclusion: Nonradioactive DNA in situ hybridization is an easy and efficient method to be performed for the diagnosis of HPV infections. Koilocytosis with atypy is directly correlated with HPV infection and it is suggested to monitor the CIN cases with HPV type 16/18 infection since the pathology can be progressive.en_US
dc.description.sponsorshipFederat European Microbiol Socen_US
dc.identifier.doi10.1016/0928-0197(96)00226-7en_US
dc.identifier.endpage223en_US
dc.identifier.issn0928-0197
dc.identifier.issue02.Maren_US
dc.identifier.pmid15566883en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage219en_US
dc.identifier.urihttps://doi.org/10.1016/0928-0197(96)00226-7
dc.identifier.urihttps://hdl.handle.net/11454/35167
dc.identifier.volume5en_US
dc.identifier.wosWOS:A1996UW22400019en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofClinical and Diagnostic Virologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjecthuman papillomavirusen_US
dc.subjectHPV DNAen_US
dc.subjectin situ hybridizationen_US
dc.titleDNA insitu hybridization in the diagnosis of human papillomavirus infectionen_US
dc.typeArticleen_US

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