DNA insitu hybridization in the diagnosis of human papillomavirus infection
Küçük Resim Yok
Tarih
1996
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: Certain types of human papillomavirus (HPV) are shown to be associated with the development of genital lesions. DNA hybridization methods are used for the diagnosis of HPV infections. Objective: To use a nonradioactive DNA in situ hybridization system for the investigation of HPV infections responsible for the development of genital lesions in women. Study design: Sections from archival paraffin embedded biopsy specimens of 59 cases were screened for the presence of HPV DNA sequences by using digoxigenin labeled DNA probe which is specific for all types of HPVs and digoxigenin detection system. The study group consisted of samples diagnosed as squamous hyperplasia of the vulva (group 1), koilocytosis (group 2), condyloma acuminatum/koilocytotic atypy (group 3), cervical intraepithelial neoplasia (CIN), and epidermoid carcinoma (group 4). Results: No HPV DNA was detected in groups 1 and 2 which consisted of 3 and 13 specimens respectively. Seven of 11 (63.6%) specimens in group 3 and 7 of 32 (21.9%) in group 4 were found to be positive for in situ HPV DNA. Seven positive samples in group 3 and one positive sample in group 4 were typed as HPV 6/11. Five samples of the remaining positives in group 4 were typed as HPV 16/18. One case was found to be positive with both 16/18 and 31/33. Conclusion: Nonradioactive DNA in situ hybridization is an easy and efficient method to be performed for the diagnosis of HPV infections. Koilocytosis with atypy is directly correlated with HPV infection and it is suggested to monitor the CIN cases with HPV type 16/18 infection since the pathology can be progressive.
Açıklama
International Symposium on Recent Advances in the Diagnosis of Viral Diseases -- JUL 20-22, 1995 -- ISTANBUL, TURKEY
Anahtar Kelimeler
human papillomavirus, HPV DNA, in situ hybridization
Kaynak
Clinical and Diagnostic Virology
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
5
Sayı
02.Mar
