Noncovalently galactose imprinted polymer for the recognition of different saccharides
Küçük Resim Yok
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Molecularly imprinted polymers (MIPs) represent a new class of materials possessing high selectivity and affinity for the target molecule. The main goal of this study was to prepare a galactose imprinted polymer and its potential application for the recognition of different saccharides. The selectivity of galactose imprinted polymer for several saccharides: glucose, mannose, fructose, maltose, lactose, Sucrose and raffinose was investigated. Macroporous polymer was prepared utilizing ethyleneglycoldimethacrylate as a crosslinking agent, in the presence of galactose as a template molecule with acrylamide as a functional monomer. After the synthesis of polymer, galactose was removed by methanol:acetic acid washing. The selectivity of galactose imprinted polymer for other saccharides was utilized by batch rebinding assay. The arrangement of functional groups within cavities versus shape selectivity is discussed. The results showed the orientation of the functional groups was the dominating factor for the selectivity of galactose that, imprinted polymer. The dissociation constants of polymer were determined by Scatchard analysis. (c) 2009 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Molecular imprinting, Molecularly imprinted polymer, Galactose imprinting, Carbohydrate, Shape selectivity
Kaynak
Talanta
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
78
Sayı
3
