Blockade of PD-1/PD-L1 Axis May Improve NK-92 CellInhibition Caused by Mesenchymal Stem Cells

Basit Öğe Kaydı
dc.contributor.authorÖzdemir, Rabia Bilge Özgül
dc.contributor.authorÖzdemir, Alper Tunga
dc.contributor.authorBerdeli, Afig
dc.contributor.authorKırmaz, Cengiz
dc.contributor.authorVural, Kamil
dc.contributor.authorTuğlu, Mehmet İbrahim
dc.contributor.authorÖztatlıcı, Mustafa
dc.date.accessioned2023-01-12T20:27:14Z
dc.date.available2023-01-12T20:27:14Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractObjectives: In this study, it was aimed to investigate how the effects of Mesenchymal stem cells (MSCs) on the anti tumor properties of NK-92 cells change with programmed death-ligand-1 (PD-L1) blocking antibodies. Methods: NK-92 cells were co-cultured with MDA-MB-231 breast tumor cells and MSCs. To evaluate the effect of anti PD-L1 antibodies, cells were cultured for 48 hours with and without the addition of 1, 5, and 10 µg/ml anti PD-L1antibody. IFN-?, TNF-?, IL-10 and IDO levels of medium supernatants were determined by ELISA. CCK-8 kit was used toevaluate cytotoxic activity. Results: IFN-? and TNF-? expressions of NK-92 cells co-cultured with MDA-MB-231 increased significantly, but thisincrease was significantly decreased in culture groups with MSCs. IDO expressions increased significantly in co-culturegroups with MSCs only. Cytotoxic effects of NK-92 cells were significantly reduced in culture groups with MSCs. How ever, the suppression effects caused by MSCs improved in the presence of anti-PD-L1 antibodies and in a dose depen dent manner. Conclusion: In our findings, we found that MSCs are a highly effective inhibitors, and the IDO enzyme they secrete mayplay a major role in this. However, the suppressive effects caused by MSCs may be significantly improved by blockingthe PD-1/PD-L1 axis.en_US
dc.identifier.doi10.14744/ejmi.2021.49785
dc.identifier.endpage340en_US
dc.identifier.issn2602-3164
dc.identifier.issue3en_US
dc.identifier.startpage334en_US
dc.identifier.trdizinid491628en_US
dc.identifier.urihttps://doi.org/10.14744/ejmi.2021.49785
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/491628
dc.identifier.urihttps://hdl.handle.net/11454/80219
dc.identifier.volume5en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofEurasian Journal of Medical Investigationen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleBlockade of PD-1/PD-L1 Axis May Improve NK-92 CellInhibition Caused by Mesenchymal Stem Cellsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu