Blockade of PD-1/PD-L1 Axis May Improve NK-92 CellInhibition Caused by Mesenchymal Stem Cells
Küçük Resim Yok
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objectives: In this study, it was aimed to investigate how the effects of Mesenchymal stem cells (MSCs) on the anti tumor properties of NK-92 cells change with programmed death-ligand-1 (PD-L1) blocking antibodies. Methods: NK-92 cells were co-cultured with MDA-MB-231 breast tumor cells and MSCs. To evaluate the effect of anti PD-L1 antibodies, cells were cultured for 48 hours with and without the addition of 1, 5, and 10 µg/ml anti PD-L1antibody. IFN-?, TNF-?, IL-10 and IDO levels of medium supernatants were determined by ELISA. CCK-8 kit was used toevaluate cytotoxic activity. Results: IFN-? and TNF-? expressions of NK-92 cells co-cultured with MDA-MB-231 increased significantly, but thisincrease was significantly decreased in culture groups with MSCs. IDO expressions increased significantly in co-culturegroups with MSCs only. Cytotoxic effects of NK-92 cells were significantly reduced in culture groups with MSCs. How ever, the suppression effects caused by MSCs improved in the presence of anti-PD-L1 antibodies and in a dose depen dent manner. Conclusion: In our findings, we found that MSCs are a highly effective inhibitors, and the IDO enzyme they secrete mayplay a major role in this. However, the suppressive effects caused by MSCs may be significantly improved by blockingthe PD-1/PD-L1 axis.
Açıklama
Anahtar Kelimeler
Kaynak
Eurasian Journal of Medical Investigation
WoS Q Değeri
Scopus Q Değeri
Cilt
5
Sayı
3
