Investigation of Bioactivity of Estragole Isolated from Basil Plant on Brain Cancer Cell Lines Using Nuclear Method
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bentham Science Publ Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background In recent years, there has been a significant increase in studies investigating the potential use of plant-origin products in the treatment and diagnosis of different types of cancer. Methods In this study, Estragole (EST) was isolated from basil leaves via ethanolic extraction using an 80% ethanol concentration. The isolation process was performed using the High Performance Liquid Chromatography (HPLC) method. The EST isolated from the basil plant was radiolabeled with I-131 using the iodogen method. Quality control studies of the radiolabeled EST (I-131-EST) were carried out by using Thin Layer Radio Chromatography (TLRC). Next, in vitro cell, culture studies were done to investigate the bio-affinity of plant-originated EST labeled with I-131 on human medulloblastoma (DAOY) and human glioblastoma-astrocytoma (U-87 MG) cell lines. Finally, the cytotoxicity of EST was determined, and cell uptake of I-131-EST was investigated on cancer cell lines by incorporation studies. Results As a result of these studies, it has been shown that I-131-EST has a significant uptake on the brain cells. Conclusion This result is very satisfying, and it has encouraged us to do in vivo studies for the molecule in the future.
Açıklama
Anahtar Kelimeler
Basil plant, estragole (EST), iodine-131 (I-131), estragole radiolabeled with I-131 (I-131-EST), human medulloblastoma (DAOY), human glioblastoma-astrocytoma (U87-MG), Medicinal-Plants, Biodistribution, Leaves, I-131, Radioiodination, Perspective, Morphine
Kaynak
Current Radiopharmaceuticals
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
16
Sayı
2
