Biocompatibility of MG-63 cells on collagen, poly-L-lactic acid, hydroxyapatite scaffolds with different parameters

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dc.contributor.authorCecen, Berivan
dc.contributor.authorKozaci, Didem
dc.contributor.authorYuksel, Mithat
dc.contributor.authorErdemli, Diler
dc.contributor.authorBagriyanik, Alper
dc.contributor.authorHavitcioglu, Hasan
dc.date.accessioned2019-10-27T22:12:15Z
dc.date.available2019-10-27T22:12:15Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractPurpose: In this study, osteoblast-like MG-63 cells were cultured on 3 different scaffold types composed of (a) collagen + poly-L-lactic acid (PLLA), (b) collagen + hydroxyapatite (HA; 30 degrees C) or (c) collagen + hydroxyapatite (HA; 37 degrees C) and produced with different porosities. Methods: Biomechanical properties of the scaffolds were characterized by tensile strength measurements. Properties of the cell-seeded scaffolds were evaluated with scanning electron microscopy (SEM). Cell adhesion and proliferation capacities were evaluated. Alkaline phosphatase (ALP) levels in media were measured. Transmission electron microscopy (TEM) and histological analyses were used to assess morphological characteristics. Results: Our results showed that collagen-based PLLA and HA scaffolds have good cell biocompatibility. MTT test showed that the scaffolds exhibited no cytotoxicity. According to the force and displacement data, collagen + HA at 37 degrees C showed the highest mechanical strength and displacement. Conclusion: The results suggest that collagen-based PLLA and HA scaffolds might improve osteoblastic growth in vitro and have biomaterial integration potential in possible therapeutic approaches for future clinical studies.en_US
dc.identifier.doi10.5301/jabfm.5000182en_US
dc.identifier.endpage16en_US
dc.identifier.issn2280-8000
dc.identifier.issue1en_US
dc.identifier.pmid24744232en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage10en_US
dc.identifier.urihttps://doi.org/10.5301/jabfm.5000182
dc.identifier.urihttps://hdl.handle.net/11454/49313
dc.identifier.volume13en_US
dc.identifier.wosWOS:000362370500002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWichtig Publishingen_US
dc.relation.ispartofJournal of Applied Biomaterials & Functional Materialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCollagenen_US
dc.subjectHydroxyapatiteen_US
dc.subjectMG-63en_US
dc.subjectPoly-L-lactic aciden_US
dc.subjectScaffolden_US
dc.titleBiocompatibility of MG-63 cells on collagen, poly-L-lactic acid, hydroxyapatite scaffolds with different parametersen_US
dc.typeArticleen_US

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