Biocompatibility of MG-63 cells on collagen, poly-L-lactic acid, hydroxyapatite scaffolds with different parameters

Küçük Resim Yok

Tarih

2015

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Wichtig Publishing

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Purpose: In this study, osteoblast-like MG-63 cells were cultured on 3 different scaffold types composed of (a) collagen + poly-L-lactic acid (PLLA), (b) collagen + hydroxyapatite (HA; 30 degrees C) or (c) collagen + hydroxyapatite (HA; 37 degrees C) and produced with different porosities. Methods: Biomechanical properties of the scaffolds were characterized by tensile strength measurements. Properties of the cell-seeded scaffolds were evaluated with scanning electron microscopy (SEM). Cell adhesion and proliferation capacities were evaluated. Alkaline phosphatase (ALP) levels in media were measured. Transmission electron microscopy (TEM) and histological analyses were used to assess morphological characteristics. Results: Our results showed that collagen-based PLLA and HA scaffolds have good cell biocompatibility. MTT test showed that the scaffolds exhibited no cytotoxicity. According to the force and displacement data, collagen + HA at 37 degrees C showed the highest mechanical strength and displacement. Conclusion: The results suggest that collagen-based PLLA and HA scaffolds might improve osteoblastic growth in vitro and have biomaterial integration potential in possible therapeutic approaches for future clinical studies.

Açıklama

Anahtar Kelimeler

Collagen, Hydroxyapatite, MG-63, Poly-L-lactic acid, Scaffold

Kaynak

Journal of Applied Biomaterials & Functional Materials

WoS Q Değeri

Q4

Scopus Q Değeri

Q3

Cilt

13

Sayı

1

Künye