Biodistribution of radiolabeled alpha-amanitin in mice: An Investigation
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pergamon-Elsevier Science Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Mushroom poisonings caused by Amanita phalloides are the leading cause of mushroom -related deaths worldwide. Alpha-Amanitin (alpha-AMA), a toxic substance present in these mushrooms, is responsible for the resulting hepatotoxicity and nephrotoxicity. The objective of our study was to determine the distribution of alpha-AMA in Balb/c mice by labeling with Iodine -131. Mice were injected with a toxic dose (1.4 mg/kg) of alpha-AMA labeled with Iodine -131. The mice were sacrificed at the 1st, 2nd, 4th, 8th, 24th, and 48th hours under anesthesia. The organs of the mice were removed, and their biodistribution was assessed in all experiments. The percent injected dose per gram (ID/g %) value for kidney, liver, lung, and heart tissues at 1st hour were 1.59 +/- 0.07, 1.25 +/- 0.33, 3.67 +/- 0.80 and 1.07 +/- 0.01 respectively. This study provides insights into the potential long-term effects of alpha-AMA accumulation in specific organs. Additionally, this study has generated essential data that can be used to demonstrate the impact of antidotes on the biological distribution of alpha-AMA in future toxicity models.
Açıklama
Anahtar Kelimeler
Alpha-Amanitin, Amanita Phalloides, Toxicity, In Vivo Biodistribution, I-131
Kaynak
Toxicon
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
241
