The expression of HDAC9 and P300 in papillary thyroid carcinoma cell line

Basit Öğe Kaydı
dc.authorscopusid56548536700
dc.authorscopusid56301503200
dc.authorscopusid53664427700
dc.authorscopusid56502085400
dc.authorscopusid26022436600
dc.authorscopusid6505803191
dc.authorscopusid12041941200
dc.contributor.authorOzisik, H.
dc.contributor.authorOzdil, B.
dc.contributor.authorSuner, A.
dc.contributor.authorSipahi, M.
dc.contributor.authorErdogan, M.
dc.contributor.authorCetinkalp, S.
dc.contributor.authorOzgen, G.
dc.date.accessioned2024-08-25T18:36:03Z
dc.date.available2024-08-25T18:36:03Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractPurpose: Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer and accounts for 85–90% of all thyroid cancers. Metastatic differentiated thyroid cancer, radioiodine-refractory thyroid cancer, and anaplastic thyroid cancer still lack effective therapeutic options. Here, we aimed to assess HDAC9 and P300 expression in the papillary thyroid carcinoma cell line and compare them with normal thyroid cells. Methods: Nthy-ori-3–1, a normal thyroid cell line, and BCPAP, a PTC cell line, were cultured for 24 and 48 h and immunofluorescence staining was used to determine the levels of HDAC9 and P300 protein expression. HDAC9 paracrine release was assessed using an ELISA assay. Results: HDAC9 protein expression was higher in both cell groups at the 48th hour than at the 24th hour; however, P300 protein expression was lower in BCPAP cells at the 48th hour than at the 24th hour. In comparison to Nthy-ori-3–1, BCPAP expressed more HDAC9 and P300 proteins. HDAC9 secretion slightly increased in Nthy-ori-3–1 cells from 24 to 48 h. Furthermore, HDAC9 secretion in BCPAP cells dramatically decreased from 24 to 48 h. Conclusion: Our findings revealed that the expression of HDAC9 and P300 was higher in the PTC cell line than in normal thyroid cells. This indicates that the acetylation mechanism in thyroid cancer cells is not the same as it is in healthy cells. Epigenetic studies may reveal the mechanisms underlying PTC with further analysis. © 2023 Elsevier GmbHen_US
dc.description.sponsorshipWe would like to thank to Ahmet Topacoglu for his linguistic revision. Not applicable.en_US
dc.identifier.doi10.1016/j.prp.2023.154385
dc.identifier.issn0344-0338
dc.identifier.pmid36857949en_US
dc.identifier.scopus2-s2.0-85149064343en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.prp.2023.154385
dc.identifier.urihttps://hdl.handle.net/11454/100507
dc.identifier.volume243en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier GmbHen_US
dc.relation.ispartofPathology Research and Practiceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240825_Gen_US
dc.subjectHDAC9en_US
dc.subjectP300en_US
dc.subjectPapillary thyroid canceren_US
dc.subjectE1A associated p300 proteinen_US
dc.subjecthistone deacetylase 9en_US
dc.subjectHDAC9 protein, humanen_US
dc.subjecthistone deacetylaseen_US
dc.subjectradioactive iodineen_US
dc.subjectrepressor proteinen_US
dc.subjectArticleen_US
dc.subjectB-CPAP cell lineen_US
dc.subjectcontrolled studyen_US
dc.subjectenzyme linked immunosorbent assayen_US
dc.subjectenzyme releaseen_US
dc.subjecthistone acetylationen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectimmunocytochemistryen_US
dc.subjectNthy-ori 3-1 cell lineen_US
dc.subjectparacrine signalingen_US
dc.subjectprotein expressionen_US
dc.subjectprotein expression levelen_US
dc.subjectthyroid papillary carcinomaen_US
dc.subjectthyroid papillary carcinoma cell lineen_US
dc.subjectcell proliferationen_US
dc.subjectpapillary carcinomaen_US
dc.subjectpathologyen_US
dc.subjectthyroid tumoren_US
dc.subjecttumor cell lineen_US
dc.subjectCarcinoma, Papillaryen_US
dc.subjectCell Line, Tumoren_US
dc.subjectCell Proliferationen_US
dc.subjectHistone Deacetylasesen_US
dc.subjectHumansen_US
dc.subjectIodine Radioisotopesen_US
dc.subjectRepressor Proteinsen_US
dc.subjectThyroid Cancer, Papillaryen_US
dc.subjectThyroid Neoplasmsen_US
dc.titleThe expression of HDAC9 and P300 in papillary thyroid carcinoma cell lineen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu