The expression of HDAC9 and P300 in papillary thyroid carcinoma cell line
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier GmbH
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose: Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer and accounts for 85–90% of all thyroid cancers. Metastatic differentiated thyroid cancer, radioiodine-refractory thyroid cancer, and anaplastic thyroid cancer still lack effective therapeutic options. Here, we aimed to assess HDAC9 and P300 expression in the papillary thyroid carcinoma cell line and compare them with normal thyroid cells. Methods: Nthy-ori-3–1, a normal thyroid cell line, and BCPAP, a PTC cell line, were cultured for 24 and 48 h and immunofluorescence staining was used to determine the levels of HDAC9 and P300 protein expression. HDAC9 paracrine release was assessed using an ELISA assay. Results: HDAC9 protein expression was higher in both cell groups at the 48th hour than at the 24th hour; however, P300 protein expression was lower in BCPAP cells at the 48th hour than at the 24th hour. In comparison to Nthy-ori-3–1, BCPAP expressed more HDAC9 and P300 proteins. HDAC9 secretion slightly increased in Nthy-ori-3–1 cells from 24 to 48 h. Furthermore, HDAC9 secretion in BCPAP cells dramatically decreased from 24 to 48 h. Conclusion: Our findings revealed that the expression of HDAC9 and P300 was higher in the PTC cell line than in normal thyroid cells. This indicates that the acetylation mechanism in thyroid cancer cells is not the same as it is in healthy cells. Epigenetic studies may reveal the mechanisms underlying PTC with further analysis. © 2023 Elsevier GmbH
Açıklama
Anahtar Kelimeler
HDAC9, P300, Papillary thyroid cancer, E1A associated p300 protein, histone deacetylase 9, HDAC9 protein, human, histone deacetylase, radioactive iodine, repressor protein, Article, B-CPAP cell line, controlled study, enzyme linked immunosorbent assay, enzyme release, histone acetylation, human, human cell, immunocytochemistry, Nthy-ori 3-1 cell line, paracrine signaling, protein expression, protein expression level, thyroid papillary carcinoma, thyroid papillary carcinoma cell line, cell proliferation, papillary carcinoma, pathology, thyroid tumor, tumor cell line, Carcinoma, Papillary, Cell Line, Tumor, Cell Proliferation, Histone Deacetylases, Humans, Iodine Radioisotopes, Repressor Proteins, Thyroid Cancer, Papillary, Thyroid Neoplasms
Kaynak
Pathology Research and Practice
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
243
