RT-PCR-based cytochrome P450 expression profile of oral tissue samples
Küçük Resim Yok
Tarih
2007
Yazarlar
Sarikaya, D.
Chiba, I.
Bilgen, C.
Kamatakil, T., I
Topcu, Z.
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Blackwell Publishing
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: To analyse the expression of individual forms of cytochrome P450 (CYP) at the mRNA level in five homogenized oral buccal tissue samples from four individuals with or without oral malignancy. Method: Individual forms of CYPs were studied by reverse transcriptase-polymerase chain reaction (RT-PCR), using specific primers for CYPs 2B6, 2C, 2D6, 2E1, 3A3/4 and 3A5, and oral CYP expressions were compared with CYP expression in liver tissue. Results: Consistent expression of CYPs 2C, 2E1 and 3A5 was observed in oral buccal tissue at mRNA level. Conclusions: These particular CYPs have possible roles in the protection of the body against orally ingested xenobiotics as well as influence the bioavailability of therapeutic compounds.
Açıklama
Anahtar Kelimeler
cytochrome P450, drug metabolizing enzymes, oral buccal tissue
Kaynak
Journal of Clinical Pharmacy and Therapeutics
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
32
Sayı
5
