Long-term Stability of Cationic Phytosphingosine Nanoemulsions as Delivery Systems for plasmid DNA

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dc.contributor.authorAkbaba, Hasan
dc.contributor.authorBaşpınar, Yücel
dc.contributor.authorErel-Akbaba, Gülşah
dc.contributor.authorİsar, Selen
dc.contributor.authorNalbantsoy, Ayşe
dc.contributor.authorŞahin, Yiğit
dc.date.accessioned2023-01-12T20:29:17Z
dc.date.available2023-01-12T20:29:17Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractGene delivery systems like cationic nanoemulsions are in general not ready to use for any time. Thus, delivery systems for genetic materials like nucleic acids have to be prepared freshly before application. This study is focused on the preparation and characterization of cationic nanoemulsions using phytosphingosine for plasmid DNA delivery. Repurposing of cationic agents guided us to phytosphingosine, previously used for enhanced interaction with negatively charged surfaces. It was reported that phytosphingosine may act anti-apoptotic, but without using it in an appropriate delivery system like nanoemulsions. This gap attracted our interest in preparing and characterizing long-term stable cationic nanoemulsions and their cytotoxic effects on MDA-MB-231 and MCF-7 breast cancer cells using phytosphingosine. The cationic nanoemulsions 1, 2, and 3 were prepared and characterized in terms of droplet size, polydispersity index, zeta potential, long-term stability after storage at 25 and 40 °C, complexation with pDNA, release and cytotoxicity on MDA-MB-231 and MCF-7 cells. The CNEs showed appropriate properties like a small droplet size (<200 nm), a narrow size distribution and a high zeta potential (>+30 mV). Unfortunately, each cationic nanoemulsion showed some disadvantages. Cationic nanoemulsion 1 decreased the viability of cancer cells to only 25 %. Phase separation was observed for cationic nanoemulsion 2 after storage of six months at 40 °C. And cationic nanoemulsion 3 was not able to form a complex with pDNA. To sum up, cationic nanoemulsion 1 is more appropriate than the other cationic nanoemulsions for delivering pDNA.en_US
dc.identifier.doi10.18466/cbayarfbe.948114
dc.identifier.endpage118en_US
dc.identifier.issn1305-130X
dc.identifier.issn1305-1385
dc.identifier.issue1en_US
dc.identifier.startpage107en_US
dc.identifier.trdizinid511206en_US
dc.identifier.urihttps://doi.org/10.18466/cbayarfbe.948114
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/511206
dc.identifier.urihttps://hdl.handle.net/11454/80422
dc.identifier.volume18en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofCelal Bayar Üniversitesi Fen Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcytotoxicityen_US
dc.subjectcationic nanoemulsionen_US
dc.subjectpDNAen_US
dc.subjectstabilityen_US
dc.subjectphytosphingosineen_US
dc.subjectcomplexationen_US
dc.titleLong-term Stability of Cationic Phytosphingosine Nanoemulsions as Delivery Systems for plasmid DNAen_US
dc.typeArticleen_US

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