Long-term Stability of Cationic Phytosphingosine Nanoemulsions as Delivery Systems for plasmid DNA
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Gene delivery systems like cationic nanoemulsions are in general not ready to use for any time. Thus, delivery systems for genetic materials like nucleic acids have to be prepared freshly before application. This study is focused on the preparation and characterization of cationic nanoemulsions using phytosphingosine for plasmid DNA delivery. Repurposing of cationic agents guided us to phytosphingosine, previously used for enhanced interaction with negatively charged surfaces. It was reported that phytosphingosine may act anti-apoptotic, but without using it in an appropriate delivery system like nanoemulsions. This gap attracted our interest in preparing and characterizing long-term stable cationic nanoemulsions and their cytotoxic effects on MDA-MB-231 and MCF-7 breast cancer cells using phytosphingosine. The cationic nanoemulsions 1, 2, and 3 were prepared and characterized in terms of droplet size, polydispersity index, zeta potential, long-term stability after storage at 25 and 40 °C, complexation with pDNA, release and cytotoxicity on MDA-MB-231 and MCF-7 cells. The CNEs showed appropriate properties like a small droplet size (<200 nm), a narrow size distribution and a high zeta potential (>+30 mV). Unfortunately, each cationic nanoemulsion showed some disadvantages. Cationic nanoemulsion 1 decreased the viability of cancer cells to only 25 %. Phase separation was observed for cationic nanoemulsion 2 after storage of six months at 40 °C. And cationic nanoemulsion 3 was not able to form a complex with pDNA. To sum up, cationic nanoemulsion 1 is more appropriate than the other cationic nanoemulsions for delivering pDNA.
Açıklama
Anahtar Kelimeler
cytotoxicity, cationic nanoemulsion, pDNA, stability, phytosphingosine, complexation
Kaynak
Celal Bayar Üniversitesi Fen Bilimleri Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
18
Sayı
1
