Boronate affinity nanoparticles for RNA isolation

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dc.contributor.authorToprak, Aykut
dc.contributor.authorGorgun, Cansu
dc.contributor.authorKuru, Cansu Ilke
dc.contributor.authorTurkcan, Ceren
dc.contributor.authorUygun, Murat
dc.contributor.authorAkgol, Sinan
dc.date.accessioned2019-10-27T19:53:47Z
dc.date.available2019-10-27T19:53:47Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this presented paper, boronic acid incorporated poly(HEMA) based nanoparticles were synthesized for RNA adsorption. For this purpose, poly(HEMA) nanopartides were synthesized by using the surfactant free emulsion polymerization technique. Then, nanoparticles were modified with 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) and functionalized with phenylboronic acid (PBA). Prepared nanoparticles were characterized with SEM, FUR and zeta-size. Optimum RNA adsorption conditions were investigated with different pHs, temperatures and initial RNA concentrations in order to determine the maximum RNA adsorption onto poly(HEMA)-IMEO-PBA nanoparticles. It was also studied that, synthesized nanoparticles could be used for 5 successive reuses and adsorption capacity of the nanoparticles decreased only about 5% at the end of the 5 cycles. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.msec.2014.11.033en_US
dc.identifier.endpage256en_US
dc.identifier.issn0928-4931
dc.identifier.issn1873-0191
dc.identifier.pmid25746268en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage251en_US
dc.identifier.urihttps://doi.org/10.1016/j.msec.2014.11.033
dc.identifier.urihttps://hdl.handle.net/11454/40338
dc.identifier.volume50en_US
dc.identifier.wosWOS:000351804100031en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofMaterials Science & Engineering C-Materials For Biological Applicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRNAen_US
dc.subjectBoronate affinityen_US
dc.subjectNanoparticleen_US
dc.titleBoronate affinity nanoparticles for RNA isolationen_US
dc.typeArticleen_US

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