Boronate affinity nanoparticles for RNA isolation
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this presented paper, boronic acid incorporated poly(HEMA) based nanoparticles were synthesized for RNA adsorption. For this purpose, poly(HEMA) nanopartides were synthesized by using the surfactant free emulsion polymerization technique. Then, nanoparticles were modified with 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) and functionalized with phenylboronic acid (PBA). Prepared nanoparticles were characterized with SEM, FUR and zeta-size. Optimum RNA adsorption conditions were investigated with different pHs, temperatures and initial RNA concentrations in order to determine the maximum RNA adsorption onto poly(HEMA)-IMEO-PBA nanoparticles. It was also studied that, synthesized nanoparticles could be used for 5 successive reuses and adsorption capacity of the nanoparticles decreased only about 5% at the end of the 5 cycles. (C) 2014 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
RNA, Boronate affinity, Nanoparticle
Kaynak
Materials Science & Engineering C-Materials For Biological Applications
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
50
