Activity and stability enhancement of ?-amylase treated with sub- and supercritical carbon dioxide

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dc.contributor.authorSenyay-Oncel D.
dc.contributor.authorYesil-Celiktas O.
dc.date.accessioned2019-10-27T08:33:27Z
dc.date.available2019-10-27T08:33:27Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractVarious physical, chemical and genetic approaches have been applied in order to enhance enzyme stability and activity. In this study, the aim was to investigate the capability of sub- and supercritical carbon dioxide to alter the stability and activity of ?-amylase as an alternative technique. The effects of operational parameters such as pressure (50-300bar), temperature (28-80°C), CO 2 flow (2-10gmin -1) and time (60-180min) were evaluated in regard to the activity and stability of fungal based ?-amylase from Aspergillus oryzea. The activity of untreated enzyme was determined as 17,726µmol/ml/min. While both sub- and supercritical conditions enhanced the activity, the increase in flow rate had an adverse effect and the activity was decreased by 28.9% at a flow rate of 10gmin -1 under supercritical conditions. Nuclear magnetic resonance (NMR) spectra of untreated enzyme and treated samples exhibiting the lowest and the highest activities were almost identical except for the chemical shifts observed at the lowest activity sample from 4.0 to 4.4ppm which were assigned to protons of hydrogen-bonded groups. Optimum conditions were determined as 240bar, 41°C, 4gmin -1 CO 2 flow and 150min of process duration yielding 67.7% (29,728µmol/ml/min) higher activity than the untreated enzyme providing fundamental basis for enzymatic applications. © 2011 The Society for Biotechnology, Japan.en_US
dc.identifier.doi10.1016/j.jbiosc.2011.07.012en_US
dc.identifier.endpage440en_US
dc.identifier.issn1389-1723
dc.identifier.issue5en_US
dc.identifier.pmid21824817en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage435en_US
dc.identifier.urihttps://doi.org/10.1016/j.jbiosc.2011.07.012
dc.identifier.urihttps://hdl.handle.net/11454/26778
dc.identifier.volume112en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Bioscience and Bioengineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmylaseen_US
dc.subjectEnzyme activityen_US
dc.subjectNuclear magnetic resonance (NMR)en_US
dc.subjectOptimizationen_US
dc.subjectScanning electron microscopy (SEM)en_US
dc.subjectSupercritical fluid processsingen_US
dc.titleActivity and stability enhancement of ?-amylase treated with sub- and supercritical carbon dioxideen_US
dc.typeArticleen_US

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