Potential neuroprotective effect of ?-glutamylcysteine ethyl ester on rat brain against kainic acid-induced excitotoxicity

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dc.contributor.authorYalcin A.
dc.contributor.authorArmagan G.
dc.contributor.authorTurunc E.
dc.contributor.authorKonyalioglu S.
dc.contributor.authorKanit L.
dc.date.accessioned2019-10-27T08:34:57Z
dc.date.available2019-10-27T08:34:57Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this study was to investigate the effect of ?- Glutamylcysteine Ethyl Ester (GCEE) on the levels of GSH, caspase-3 activity, DNA damage and the expressions of Bcl-2, Bax and p53 mRNAs in rat hippocampus after status epilepticus (SE) induced by systemic kainic acid (KA). The male rats were divided into four groups as controls, KA (10 mg/kg), GCEE (10 mg/kg) and KAGCEE. Glutathione (GSH) levels and caspase-3 activity were determined spectrophotometrically and colourimetrically, respectively. DNA damage and Bcl-2, Bax and p53 mRNA expressions were quantifi ed by comet assay and reverse transcription followed by RT-PCR, respectively. KA treatment signifi cantly depleted GSH levels, induced DNA damage, caspase-3 activity and the expressions of p53 and Bax mRNA. GCEE treatment protected GSH levels, decreased DNA damage and the levels of p53 and Bax/Bcl-2 mRNA against KA injection. These results indicate that GCEE treatment at the dose of 10 mg/kg is capable to protect the depleted levels of GSH and shows an anti-apoptotic activity due to the decreased levels of apoptotic biomarkers in the rat hippocampus after SE induced by KA. © 2010 Informa UK Ltd. (Informa Healthcare, Taylor & Francis AS).en_US
dc.identifier.doi10.3109/10715761003645964en_US
dc.identifier.endpage521en_US
dc.identifier.issn1071-5762
dc.identifier.issue5en_US
dc.identifier.pmid20214503en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage513en_US
dc.identifier.urihttps://doi.org/10.3109/10715761003645964
dc.identifier.urihttps://hdl.handle.net/11454/27143
dc.identifier.volume44en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofFree Radical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject?-glutamylcysteine ethyl ester (GCEE)en_US
dc.subjectBax mRNAen_US
dc.subjectBcl-2 mRNAen_US
dc.subjectCaspase-3 activityen_US
dc.subjectDNA damageen_US
dc.subjectKainic aciden_US
dc.subjectP53 mRNAen_US
dc.titlePotential neuroprotective effect of ?-glutamylcysteine ethyl ester on rat brain against kainic acid-induced excitotoxicityen_US
dc.typeArticleen_US

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