Potential neuroprotective effect of ?-glutamylcysteine ethyl ester on rat brain against kainic acid-induced excitotoxicity
Küçük Resim Yok
Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study was to investigate the effect of ?- Glutamylcysteine Ethyl Ester (GCEE) on the levels of GSH, caspase-3 activity, DNA damage and the expressions of Bcl-2, Bax and p53 mRNAs in rat hippocampus after status epilepticus (SE) induced by systemic kainic acid (KA). The male rats were divided into four groups as controls, KA (10 mg/kg), GCEE (10 mg/kg) and KAGCEE. Glutathione (GSH) levels and caspase-3 activity were determined spectrophotometrically and colourimetrically, respectively. DNA damage and Bcl-2, Bax and p53 mRNA expressions were quantifi ed by comet assay and reverse transcription followed by RT-PCR, respectively. KA treatment signifi cantly depleted GSH levels, induced DNA damage, caspase-3 activity and the expressions of p53 and Bax mRNA. GCEE treatment protected GSH levels, decreased DNA damage and the levels of p53 and Bax/Bcl-2 mRNA against KA injection. These results indicate that GCEE treatment at the dose of 10 mg/kg is capable to protect the depleted levels of GSH and shows an anti-apoptotic activity due to the decreased levels of apoptotic biomarkers in the rat hippocampus after SE induced by KA. © 2010 Informa UK Ltd. (Informa Healthcare, Taylor & Francis AS).
Açıklama
Anahtar Kelimeler
?-glutamylcysteine ethyl ester (GCEE), Bax mRNA, Bcl-2 mRNA, Caspase-3 activity, DNA damage, Kainic acid, P53 mRNA
Kaynak
Free Radical Research
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
44
Sayı
5
