Melatonin tirotoksikozlu sıçanlarda nitrik oksit yanıtını baskılar
Küçük Resim Yok
Tarih
2008
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Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Nitrik oksitin deneysel hipertiroidizmdeki rolünü ve melatoninin nitrik oksit yanıtına etkisini araştırmak. Durum Değerlendirilmesi: Hipertiroidizm, serbest oksijen radikali üretimine, serbest oksijen radikalleri de hücrede hasara neden olmaktadır. Metabolizma düzenlenmesinde etkin rol oynayan bir hormon olan melatonin, direkt antioksidan etkisiyle nitrik oksit (NO) gibi oksidatif strese yol açan serbest radikallerin biyomoleküller üzerindeki zararlı etkilerini önler. Gereç ve Yöntem: Çalışmada 30 adet Wistar Albino dişi sıçan üç grup olarak randomize edildi. Grup A'ya (negatif kontrol grubu) intraperitoneal serum fizyolojik; Grup B'ye (pozitif kontrol grubu) intraperitoneal 0,2 mg/kg/gün L-tiroksin ve Grup C'ye (tedavi grubu) intraperitoneal 0,2 mg/kg/gün L-tiroksinin ile birlikte intraperitoneal 3 mg/kg/gün melatonin uygulandı. Üçüncü hafta sonunda, tüm sıçanlar dekapite edilerek serum, karaciğer ve kalp dokularından örneklemeler yapıldı. Biyokimyasal olarak kan ve dokuda serbest T3 (FT3), serbest T4 (FT4), tiroid stimulan hormon (TSH) ve NO düzeyleri araştırıldı. Bulgular: Kan FT3 düzeyi açısından, Grup A ve B ile Grup B ve C arasında, istatistiksel olarak anlamlı fark izlendi (p<0.001 ve p=0.004, sırasıyla). Grup A ve B ile Grup A ve C arasında, kan FT4 düzeyleri açısından istatistiksel olarak anlamlı fark izlendi (p<0.001 ve p=0.02, sırasıyla). Median değerleri sırasıyla 5,93 ?M/L; 48,41 ?M/L ve 42,69 ?M/L olan kan NO düzeyleri de gruplar arasında anlamlı fark gösterdi (p<0.001). Kalp NO median değerilerine bakıldığında, B grubunda A grubu ile aynı (1,74 µmol/g'a karşın 1,74 µmol/g) olduğu ve bu değerin C grubunda baskılandığı (1,25 µmol/g), ancak bu yanıtın istatistiksel olarak anlamlı olmadığı görüldü (p=0,05). Karaciğer NO düzeyleri arasındaki fark ise, Grup A ve B arasında anlamlı bulunurken (p<0.001) Grup B ve C arasında anlamlı değildi (0,30 µmol/g; 0,46 µmol/g ve 0,41 µmol/g, sırasıyla). Sonuç: Bu çalışmanın, melatoninin değişik dozlarda ve/veya zaman aralıklarında uygulanımıyla yapılacak çalışmalarla doğrulanması ve desteklenmesi halinde; birçok klinik alanda kullanıma girdiği bildirilen melatonin ile hipertiroidizm gibi tüm sistemleri etkileyen patolojilerle savaşımda, yeni açılımlar sağlanabileceği inancındayız.
Purpose: Hyperthyroidism results in free radical production and this free radical production results in cell damage. Melatonin, which is an effective hormone in the management of the metabolism, prevents the harmful effects of free radicals like nitric oxide via its direct antioxidant effect. This study was conducted to investigate the role of nitric oxide in experimental hyperthyroidism and the effect of melatonin on nitric oxide. Materials and Methods: Thirty Wistar Albino female rats were randomized into three groups: Group A (negative control group) underwent saline injection; Group B (positive control group) underwent intraperitoneal L-Thyroxine 0.2 mg/kg/day and Group C (treatment group) underwent intraperitoneal L-Thyroxine 0.2 mg/kg/day + melatonin 3 mg/kg/day. After 3 weeks, all rats were decapitated and blood, liver and heart tissue samples were taken. Biochemically, FT3, FT4, TSH and NO levels were investigated. Results: There was a significant change in FT3 levels between Groups A and B, and Groups B and C (p<0.001 and p=0.004, respectively). Concerning FT4 levels, there was a significant change between Groups A and B and Groups A and C (p<0.001 and p=0.02 respectively). Changes in median blood NO levels of Group A, B and C were significantly different (5,93 &#956;M/L; 48,41 &#956;M/L and 42,69 &#956;M/L, respectively) (p<0.001). Heart NO level of Group B is similar to Group A (1,74 µmol/g vs. 1,74 µmol/g). There was also no statistically significant difference between Group B and C, although heart levels decreased in Group C (1,25 µmol/g), (p=0,05). Liver NO levels showed a significant difference between Group A and B, while there was no significant difference between Groups B and C (0,30 µmol/g; 0,46 µmol/g ve 0,41 µmol/g, respectively). Conclusion: Results of this study should be confirmed with future studies conducted with different doses and application time intervals of melatonin. We believe that these future studies will lead melatonin to a major role in the fight against pathologies like hyperthyroidism that affect many systems.
Purpose: Hyperthyroidism results in free radical production and this free radical production results in cell damage. Melatonin, which is an effective hormone in the management of the metabolism, prevents the harmful effects of free radicals like nitric oxide via its direct antioxidant effect. This study was conducted to investigate the role of nitric oxide in experimental hyperthyroidism and the effect of melatonin on nitric oxide. Materials and Methods: Thirty Wistar Albino female rats were randomized into three groups: Group A (negative control group) underwent saline injection; Group B (positive control group) underwent intraperitoneal L-Thyroxine 0.2 mg/kg/day and Group C (treatment group) underwent intraperitoneal L-Thyroxine 0.2 mg/kg/day + melatonin 3 mg/kg/day. After 3 weeks, all rats were decapitated and blood, liver and heart tissue samples were taken. Biochemically, FT3, FT4, TSH and NO levels were investigated. Results: There was a significant change in FT3 levels between Groups A and B, and Groups B and C (p<0.001 and p=0.004, respectively). Concerning FT4 levels, there was a significant change between Groups A and B and Groups A and C (p<0.001 and p=0.02 respectively). Changes in median blood NO levels of Group A, B and C were significantly different (5,93 &#956;M/L; 48,41 &#956;M/L and 42,69 &#956;M/L, respectively) (p<0.001). Heart NO level of Group B is similar to Group A (1,74 µmol/g vs. 1,74 µmol/g). There was also no statistically significant difference between Group B and C, although heart levels decreased in Group C (1,25 µmol/g), (p=0,05). Liver NO levels showed a significant difference between Group A and B, while there was no significant difference between Groups B and C (0,30 µmol/g; 0,46 µmol/g ve 0,41 µmol/g, respectively). Conclusion: Results of this study should be confirmed with future studies conducted with different doses and application time intervals of melatonin. We believe that these future studies will lead melatonin to a major role in the fight against pathologies like hyperthyroidism that affect many systems.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Ulusal Cerrahi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
24
Sayı
4
