Design of radiolabeled gelatinase inhibitor peptide (99mTc-CLP) and evaluation in rats

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dc.contributor.authorAltiparmak B.
dc.contributor.authorLambrecht F.Y.
dc.contributor.authorCitak A.
dc.date.accessioned2019-10-27T08:22:17Z
dc.date.available2019-10-27T08:22:17Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractIn malignant tissues, MMP-9 (gelatinase B, 92kDa type IV collagenase) and MMP-2 (gelatinase A, 72kDa type IV collagenase) are the most prevalent matrix metalloproteinases related to the tumor aggressiveness and metastatic potential. Since elevated levels of gelatinases are associated with poor prognosis in cancer patients, these enzymes are potential targets for tumor imaging to possibly predict metastases. In the present study, a cyclic decapeptide, CLP (Cys-Leu-Pro-Gly-His-Trp-Gly-Phe-Pro-Ser-Cys), was selected as a basic peptide because of its selective inhibitory activity toward gelatinases. The peptide was labelled with 99mTc with a radiolabelling efficiency of 94.6±4.1%. After determining the appropriate conditions for radiolabelling, a biodistribution study of radiolabelled peptide in Albino Wistar rats was done. According to biodistribution data, 99mTc-CLP showed high uptake in the lung, liver, uterus and spleen. The amount of normal tissue MMPs enzymes is known to be lower than a tumor tissue. In this connection, our findings show that matrix metalloproteinases inhibitory peptide which is CLP is labeled with 99mTc with high yield and radiolabeled peptide might be might be utilized for the imaging of gelatinase activity due to overexpression of MMP-2 and MMP-9 in tumor tissue. © 2014 Elsevier Ltd.en_US
dc.description.sponsorshipEge Üniversitesi: 2011 NBE 001 111S253en_US
dc.description.sponsorshipThe authors gratefully acknowledge the financial support received from Department of Scientific Projects at Ege University, Izmir, Turkey (2011 NBE 001) and the Scientific and Technological Research Council of Turkey , Scientific Project ( 111S253 ). --en_US
dc.identifier.doi10.1016/j.apradiso.2014.02.012en_US
dc.identifier.endpage133en_US
dc.identifier.issn0969-8043
dc.identifier.pmid24631744en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage130en_US
dc.identifier.urihttps://doi.org/10.1016/j.apradiso.2014.02.012
dc.identifier.urihttps://hdl.handle.net/11454/26221
dc.identifier.volume89en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofApplied Radiation and Isotopesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGelatinaseen_US
dc.subjectHWGF-containing peptideen_US
dc.subjectMatrix metalloproteinasesen_US
dc.subjectRadiolabeled peptideen_US
dc.titleDesign of radiolabeled gelatinase inhibitor peptide (99mTc-CLP) and evaluation in ratsen_US
dc.typeArticleen_US

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