Comparative analysis of protein profiles in skin secretions of some Rana species: Preliminary insights into antimicrobial activity
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pergamon-Elsevier Science Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Protein profiles of skin secretions of Rana dalmatina (Agile Frog), Rana macrocnemis (Uludag Frog), Rana tavasensis (Tavas Frog) and Rana holtzi (Taurus Frog) frog species belonging to the Rana genus distributed in the Anatolian region of Turkiye were determined for the first time using the Tricine-SDS-PAGE Electrophoresis method and Coomassie Brilliant Blue (CBB) staining. By the results, some peptides with mass <5 kDa were detected. Just one peptide with mass <5 kDa was found in the secretion of each R. dalmatina, R. macrocnemis, and R. tavasensis while there was two in R. holtzi secretion. The antibacterial activity of secretions was determined using plate well diffusion assay on E. coli, S. typhimurium, S. aureus, B. cereus and L. monocytogenes bacteria. R. dalmatina created the inhibition zone for S. typhimurium, S. aureus, B. cereus, and L. monocytogenes. The zones of inhibition by R. tavasensis and R. macrocnemis species secretions were observed on S. aureus, B. cereus, and L. monocytogenes. It was found that R. holtzi creates an inhibition zone only on B. cereus. The results showed that the secretion of none of the species doesn't have antibacterial activity on E. coli. The skin secretion of R. dalmatina showed the most activity against bacteria, while R. holtzi had the least.
Açıklama
Anahtar Kelimeler
Antimicrobial peptides, R. dalmatina, R. holtzi, R. macrocnemis, R. tavasensis, Tricine-SDS- PAGE
Kaynak
Toxicon
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
Sayı
Nov
Künye
Tanrıverdi O, E., Ayaz, D., & Terzi, Y. (2024). Comparative analysis of protein profiles in skin secretions of some rana species: Preliminary insights into antimicrobial activity. Toxicon (Oxford), 250, 108110.
