Laser-printed paper ELISA and hydroxyapatite immobilization for colorimetric congenital anomalies screening in saliva

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dc.authoridGhorbanizamani, Faezeh/0000-0001-8541-0540
dc.authoridMOULAHOUM, Hichem/0000-0003-3934-6415
dc.contributor.authorMoulahoum, Hichem
dc.contributor.authorGhorbanizamani, Faezeh
dc.contributor.authorTimur, Suna
dc.date.accessioned2024-08-31T07:49:23Z
dc.date.available2024-08-31T07:49:23Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: Alpha-fetoprotein (AFP) is a fetal protein that can indicate congenital anomalies such as Down syndrome and spinal canal blockage when detected at abnormal levels in pregnant women. Current AFP detection methods rely on invasive blood or serum samples, which require sophisticated equipment. From the many solutions proposed, colorimetric paper -based assays excel in point -of -care settings. The concept of paperbased ELISA (p-ELISA) enhances traditional methods, aligning with the ASSURED criteria for diagnostics in resource -limited regions. Despite success in microfluidic paper -based assay devices, laser printing remains underexplored for p-ELISA. Additionally, modifying the paper surface provides an additional layer of sensitivity enhancement. Results: In this study, we developed a novel laser -printed paper -based ELISA (LP-pELISA) for rapid, sensitive, and noninvasive detection of AFP in saliva samples. The LP-pELISA platform was fabricated by printing hydrophobic barriers on filter paper using a laser printer, followed by depositing hydroxyapatite (HAp) as an immobilization material for the antibodies. The colorimetric detection was achieved using AuNPs functionalized with anti-AFP antibodies and silver nitrate enhancement. The LP-pELISA exhibited a linear response for AFP detection in both buffer and saliva samples over a range of 1.0-800 ng mL -1 , with a limit of detection (LOD) reaching 1.0 ng mL -1 . The assay also demonstrated good selectivity, repeatability, reproducibility, and stability. The LP-pELISA was further validated by testing spiked human saliva samples, showing its potential for point -of -care diagnosis of congenital disabilities. Significance: The LP-pELISA is a noninvasive platform showcasing simplicity, cost-effectiveness, and userfriendliness, utilizing laser printing, hydroxyapatite modification, and saliva samples to efficiently detect AFP. Beyond its application for AFP, this method's versatility extends to other biomarkers, positioning it as a catalyst for the evolution of paper -based biosensors. The LP-pELISA holds promise as a transformative tool for point -ofcare diagnostics, fostering advancements in healthcare with its innovative technology.en_US
dc.description.sponsorshipRepublic of Turkey, Ministry of Development [2016K121190]; Scientific and Technological Research Institution of Turkey (TUBITAK) [123O186, 123Y190]en_US
dc.description.sponsorshipThe Republic of Turkey, Ministry of Development supported the infrastructure of EGE MATAL (Ege University/Izmir) via the 2016K121190 grant. The study was partially supported by the Scientific and Technological Research Institution of Turkey (TUBITAK grant numbers 123O186 and 123Y190) .en_US
dc.identifier.doi10.1016/j.aca.2024.342617
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.pmid38692789en_US
dc.identifier.scopus2-s2.0-85190823836en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.aca.2024.342617
dc.identifier.urihttps://hdl.handle.net/11454/104844
dc.identifier.volume1306en_US
dc.identifier.wosWOS:001233534800001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240831_Uen_US
dc.subjectHydroxyapatiteen_US
dc.subjectAunpen_US
dc.subjectPaper-Based Elisaen_US
dc.subjectBiosensoren_US
dc.subjectColorimetricen_US
dc.subjectSalivaen_US
dc.titleLaser-printed paper ELISA and hydroxyapatite immobilization for colorimetric congenital anomalies screening in salivaen_US
dc.typeArticleen_US

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