Laser-printed paper ELISA and hydroxyapatite immobilization for colorimetric congenital anomalies screening in saliva

Küçük Resim Yok

Tarih

2024

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Elsevier

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Background: Alpha-fetoprotein (AFP) is a fetal protein that can indicate congenital anomalies such as Down syndrome and spinal canal blockage when detected at abnormal levels in pregnant women. Current AFP detection methods rely on invasive blood or serum samples, which require sophisticated equipment. From the many solutions proposed, colorimetric paper -based assays excel in point -of -care settings. The concept of paperbased ELISA (p-ELISA) enhances traditional methods, aligning with the ASSURED criteria for diagnostics in resource -limited regions. Despite success in microfluidic paper -based assay devices, laser printing remains underexplored for p-ELISA. Additionally, modifying the paper surface provides an additional layer of sensitivity enhancement. Results: In this study, we developed a novel laser -printed paper -based ELISA (LP-pELISA) for rapid, sensitive, and noninvasive detection of AFP in saliva samples. The LP-pELISA platform was fabricated by printing hydrophobic barriers on filter paper using a laser printer, followed by depositing hydroxyapatite (HAp) as an immobilization material for the antibodies. The colorimetric detection was achieved using AuNPs functionalized with anti-AFP antibodies and silver nitrate enhancement. The LP-pELISA exhibited a linear response for AFP detection in both buffer and saliva samples over a range of 1.0-800 ng mL -1 , with a limit of detection (LOD) reaching 1.0 ng mL -1 . The assay also demonstrated good selectivity, repeatability, reproducibility, and stability. The LP-pELISA was further validated by testing spiked human saliva samples, showing its potential for point -of -care diagnosis of congenital disabilities. Significance: The LP-pELISA is a noninvasive platform showcasing simplicity, cost-effectiveness, and userfriendliness, utilizing laser printing, hydroxyapatite modification, and saliva samples to efficiently detect AFP. Beyond its application for AFP, this method's versatility extends to other biomarkers, positioning it as a catalyst for the evolution of paper -based biosensors. The LP-pELISA holds promise as a transformative tool for point -ofcare diagnostics, fostering advancements in healthcare with its innovative technology.

Açıklama

Anahtar Kelimeler

Hydroxyapatite, Aunp, Paper-Based Elisa, Biosensor, Colorimetric, Saliva

Kaynak

Analytica Chimica Acta

WoS Q Değeri

N/A

Scopus Q Değeri

Q1

Cilt

1306

Sayı

Künye