The effects of different intraabdominal pressure protocols in laparoscopic procedures on oxidative stress markers and morphology in rat ovaries
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wroclaw University of Medicine
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: To determine the effects of different intraabdominal pressure (IAP) on the ovaries in a laparoscopic rat model. Objectives: The aim of the study was to determine the effects on the ovaries of different intraabdominal pressures (IAP) in laparoscopic surgery in a rat model. Material and Methods: Thirty-two post-pubertal nonpregnant Sprague-Dawley rats were divided randomly into four groups. In the control group, no intraabdominal pressure (IAP) was applied. In Group Pp10 and Group Ppl5, an IAP of 10 and 15 mm Hg, respectively, were applied by carbon dioxide insufflation for 60 min, and a 30-min desufflation was carried out. In Group IPp15, a 15 mm Hg IAP was applied for 10 min, and then CO2 was desufflated for 10 min. After this ischemic preconditioning, IAP was established at 15 mm Hg for 60 min, after which CO2 was desufflated for 30 min. Erythrocyte and ovarian tissue malondialdehyde (MDA) and histopathologic damage scores were evaluated. Results: In Groups Pp10 and Ppl5, ovarian tissue MDA values were significantly increased compared to the control group. In Groups Pp10 and Pp15, erythrocyte MDA values were significantly increased when compared to Group IPp15 and the control group. Ovarian histopatological assesment scores were significantly higher in Group Ppl5 than in Groups Pp10 and IPp15. Conclusions: Pneumoperitoneum causes injuries to abdominal organ such as the ovaries. The ischemic preconditioning method is more effective in reducing oxidative stress due to laparoscopic pneumoperitoneum than lowpressure pneumoperitoneum methods.
Açıklama
Anahtar Kelimeler
Ischemic preconditioning, Laparoscopy, Oxidative stress, Pneumoperitoneum, Reperfusion
Kaynak
Advances in Clinical and Experimental Medicine
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
23
Sayı
6
