Investigation of miltefosine-model membranes interactions at the molecular level for two different PS levels modeling cancer cells

Küçük Resim Yok

Tarih

2024

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Springer/Plenum Publishers

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Miltefosine (MLT) is a broad-spectrum drug included in the alkylphospholipids (APL) used against leishmania and various types of cancer. The most crucial feature of APLs is that they are thought to only kill cancerous cells without harming normal cells. However, the molecular mechanism of action of APLs is not completely understood. The increase in the phosphatidylserine (PS) ratio is a marker showing the stage of cancer and even metastasis. The goal of this research was to investigate the molecular effects of miltefosine at the molecular level in different PS ratios. The effects of MLT on membrane phase transition, membrane orders, and dynamics were studied using DPPC/DPPS (3:1) and DPPC/DPPS (1:1) multilayer (MLV) vesicles mimicking DPPS ratio variation, Differential Scanning Calorimetry (DSC), and Fourier Transform Infrared spectroscopy (FTIR). Our findings indicate that miltefosine is evidence at the molecular level that it is directed towards the tumor cell and that the drug's effect increases with the increase of anionic lipids in the membrane depending on the stage of cancer.

Açıklama

Anahtar Kelimeler

Fourier Transformation Infrared Spectroscopy, Differential Scanning Calorimetry, Miltefosine, Dipalmitoyl Phosphatidylserine Level, Cancer

Kaynak

Journal of Bioenergetics and Biomembranes

WoS Q Değeri

N/A

Scopus Q Değeri

Q2

Cilt

56

Sayı

4

Künye