Investigation of class-d beta-lactamases causing carbapenem resistance in clinical Acinetobacter baumannii isolates
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technical Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background/aim: Acinetobacter baumannii is an important causative agent of nosocomial infections, and carbapenems have been frequently used in the treatment of these infections. This study was designed to investigate the prevalence of primary carbapenem hydrolyzing oxacillinase (CHO) types in clinical A. bumannii strains. Materials and methods: Minimum inhibitory concentration (MIC) values of 76 imipenem nonsusceptible A. baumannii strains, isolated from a tertiary care hospital, were determined by microdilution method. The clonal relationship of the isolates was analyzed with enterobacterial repetitive intergenic consensus (ERIC)-PCR, and the presence of CHO major groups (OXA-23; OXA-24, OXA-51, and OXA-58 groups) was investigated with multiplex PCR. Results: According to the ERIC-PCR patterns, the isolates were distributed in 13 different clones, the largest of which had 40 members. bla(OXA-51-group) was detected in representatives of all clones, whereas bla(OXA-23-group) was detected in representatives of all but two small clones. Additionally, the presence of bla(OXA-58-group) was discovered in the members of two small clones, whereas bla(OXA-24-group) was not encountered in any of the examined strains. Conclusion: Molecular fingerprinting revealed that most imipenem-resistant A. baumannii strains were clonally related. bla(OXA-23-group) and bla(OXA-51-group) were mostly responsible for the imipenem resistance of the examined A. baumannii strains.
Açıklama
Anahtar Kelimeler
Acinetobacter baumannii, carbapenemase, enterobacterial repetitive intergenic consensus PCR, OXA-genes
Kaynak
Turkish Journal of Medical Sciences
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
47
Sayı
5
