An injectable alginate-based hydrogel for microfluidic applications

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dc.contributor.authorAkay, Seref
dc.contributor.authorHeils, Rene
dc.contributor.authorTrieu, Hoc Khiem
dc.contributor.authorSmirnova, Irina
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2019-10-27T11:09:26Z
dc.date.available2019-10-27T11:09:26Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractThe objective of this study was to develop an injectable alginate based formulation for immobilizing enzymes into microfluidic systems. The gelation was induced upon lowering the pH by addition of D-glucono-delta-lactone (GDL) and release of Ca+ ions from solid CaCO3. The effects of GDL concentration on enzymatic activity and gelation time were investigated. The results indicated that increasing the GDL concentration increased both surface area and enzymatic activity. Also, chitosan was added to the formulation at different ratios to enhance the stability of enzyme during immobilization. For microfluidic application, 100 mu l spiral coil single channel microchip was fabricated and alginate GDL mixture containing beta-glucosidase was injected to the microchannel prior to gelation. Enzymatic conversion was performed by pumping substrate (pNPG) through the microchannel. The results indicated that the entire substrate was converted continuously during 24 h without any leakage or deactivation of immobilized enzyme. (C) 2017 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113M050]; BMBFFederal Ministry of Education & Research (BMBF) [01DL14002]; International Doctoral Research Fellowship Program of TUBITAK [2214/A]en_US
dc.description.sponsorshipThe research supports by the Scientific and Technical Research Council of Turkey (TUBITAK) through 113M050 project and BMBF through 01DL14002 project are highly appreciated. Additionally, Seref Akay acknowledges 2214/A International Doctoral Research Fellowship Program of TUBITAK.en_US
dc.identifier.doi10.1016/j.carbpol.2017.01.004en_US
dc.identifier.endpage234en_US
dc.identifier.issn0144-8617
dc.identifier.issn1879-1344
dc.identifier.pmid28189233en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage228en_US
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2017.01.004
dc.identifier.urihttps://hdl.handle.net/11454/32287
dc.identifier.volume161en_US
dc.identifier.wosWOS:000395224600026en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofCarbohydrate Polymersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlginateen_US
dc.subjectChitosanen_US
dc.subjectHydrogelen_US
dc.subjectMonolithen_US
dc.subjectMicrofabricationen_US
dc.subjectMicrofluidicsen_US
dc.titleAn injectable alginate-based hydrogel for microfluidic applicationsen_US
dc.typeArticleen_US

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