An injectable alginate-based hydrogel for microfluidic applications
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The objective of this study was to develop an injectable alginate based formulation for immobilizing enzymes into microfluidic systems. The gelation was induced upon lowering the pH by addition of D-glucono-delta-lactone (GDL) and release of Ca+ ions from solid CaCO3. The effects of GDL concentration on enzymatic activity and gelation time were investigated. The results indicated that increasing the GDL concentration increased both surface area and enzymatic activity. Also, chitosan was added to the formulation at different ratios to enhance the stability of enzyme during immobilization. For microfluidic application, 100 mu l spiral coil single channel microchip was fabricated and alginate GDL mixture containing beta-glucosidase was injected to the microchannel prior to gelation. Enzymatic conversion was performed by pumping substrate (pNPG) through the microchannel. The results indicated that the entire substrate was converted continuously during 24 h without any leakage or deactivation of immobilized enzyme. (C) 2017 Elsevier Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
Alginate, Chitosan, Hydrogel, Monolith, Microfabrication, Microfluidics
Kaynak
Carbohydrate Polymers
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
161
