Role of functionalized self-assembled peptide hydrogels inin vitrovasculogenesis

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dc.authorscopusid57226123637
dc.authorscopusid57206479915
dc.authorscopusid57221554598
dc.authorscopusid55804374300
dc.contributor.authorOnak Pulat G.
dc.contributor.authorGökmen O.
dc.contributor.authorÇevik Z.B.Y.
dc.contributor.authorKaraman O.
dc.date.accessioned2023-01-12T20:23:02Z
dc.date.available2023-01-12T20:23:02Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractFabrication of vascularized tissue constructs plays an integral role in creating clinically relevant tissues. Scaffold materials should be sufficiently vascularized to mimic functional and complex native tissues. Herein, we report the development of bioactive and biomimetic self-assembled peptide (SAP) hydrogels that allow the rapid formation of a vascular structurein vitro. The KLDLKLDLKLDL (KLD peptide) SAP was functionalized with laminin derived peptides IKVAV (V1) and YIGSR (V2) through direct coupling to mimic the natural extracellular matrix (ECM) and human umbilical endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cultured in 0.5% and 1% SAP hydrogels organized into vascularized structures. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) images proved the molecular integration of the nanofibrous structure in SAP hydrogels. The stability of SAP hydrogels was confirmed by rheological and degradation measurements. Bioactive peptide scaffolds enhanced significantly HUVEC/hMSC proliferation depicted by MTT analysis compared to KLD. Furthermore, the real time quantitative polymerase chain reaction (rt-PCR) was performed to analyse vascular gene expressions such as platelet/endothelial cell adhesion molecule-1 (PECAM-1), von Willebrand factor (vWF), and vascular endothelial cadherin (VE-cadherin). The results indicated that the KLD-V2 hydrogel significantly induced vasculogenesis in hMSC/HUVEC co-culture compared to KLD-V1, Biogelx and KLD because YIGSR in KLD-V2 promoted cell population and ECM secretion by the interaction with cells and increased vasculogenesis. Overall, the designed SAP hydrogel represents an effective scaffold for vascularization of tissue constructs with useful tissue engineering applications. © The Royal Society of Chemistry 2021.en_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK: 117S429en_US
dc.description.sponsorshipThe authors acknowledge funding from TÜBİTAK (The Scientific and Technological Research Council of Turkey) through the Research Project 117S429.en_US
dc.identifier.doi10.1039/d1sm00680k
dc.identifier.endpage6626en_US
dc.identifier.issn1744-683X
dc.identifier.issue27en_US
dc.identifier.pmid34143171en_US
dc.identifier.scopus2-s2.0-85110444327en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage6616en_US
dc.identifier.urihttps://doi.org/10.1039/d1sm00680k
dc.identifier.urihttps://hdl.handle.net/11454/79625
dc.identifier.volume17en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofSoft Matteren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAtomic force microscopyen_US
dc.subjectBiomimeticsen_US
dc.subjectCell adhesionen_US
dc.subjectCell cultureen_US
dc.subjectCell proliferationen_US
dc.subjectEndothelial cellsen_US
dc.subjectGene expressionen_US
dc.subjectGlycoproteinsen_US
dc.subjectHistologyen_US
dc.subjectHydrogelsen_US
dc.subjectNanofibersen_US
dc.subjectPeptidesen_US
dc.subjectPolymerase chain reactionen_US
dc.subjectScanning electron microscopyen_US
dc.subjectStem cellsen_US
dc.subjectTissueen_US
dc.subjectCell adhesion moleculesen_US
dc.subjectExtracellular matricesen_US
dc.subjectHuman umbilical endothelial cells (HUVECs)en_US
dc.subjectMolecular integrationsen_US
dc.subjectQuantitative polymerase chain reactionen_US
dc.subjectScanning electron microscopy imageen_US
dc.subjectSelf-assembled peptidesen_US
dc.subjectTissue engineering applicationsen_US
dc.subjectScaffolds (biology)en_US
dc.subjectpeptideen_US
dc.subjectcell differentiationen_US
dc.subjecthumanen_US
dc.subjecthydrogelen_US
dc.subjectmesenchymal stem cellen_US
dc.subjecttissue engineeringen_US
dc.subjecttissue scaffolden_US
dc.subjectCell Differentiationen_US
dc.subjectHumansen_US
dc.subjectHydrogelsen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.subjectPeptidesen_US
dc.subjectTissue Engineeringen_US
dc.subjectTissue Scaffoldsen_US
dc.titleRole of functionalized self-assembled peptide hydrogels inin vitrovasculogenesisen_US
dc.typeArticleen_US

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