Role of functionalized self-assembled peptide hydrogels inin vitrovasculogenesis
Küçük Resim Yok
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Royal Society of Chemistry
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Fabrication of vascularized tissue constructs plays an integral role in creating clinically relevant tissues. Scaffold materials should be sufficiently vascularized to mimic functional and complex native tissues. Herein, we report the development of bioactive and biomimetic self-assembled peptide (SAP) hydrogels that allow the rapid formation of a vascular structurein vitro. The KLDLKLDLKLDL (KLD peptide) SAP was functionalized with laminin derived peptides IKVAV (V1) and YIGSR (V2) through direct coupling to mimic the natural extracellular matrix (ECM) and human umbilical endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cultured in 0.5% and 1% SAP hydrogels organized into vascularized structures. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) images proved the molecular integration of the nanofibrous structure in SAP hydrogels. The stability of SAP hydrogels was confirmed by rheological and degradation measurements. Bioactive peptide scaffolds enhanced significantly HUVEC/hMSC proliferation depicted by MTT analysis compared to KLD. Furthermore, the real time quantitative polymerase chain reaction (rt-PCR) was performed to analyse vascular gene expressions such as platelet/endothelial cell adhesion molecule-1 (PECAM-1), von Willebrand factor (vWF), and vascular endothelial cadherin (VE-cadherin). The results indicated that the KLD-V2 hydrogel significantly induced vasculogenesis in hMSC/HUVEC co-culture compared to KLD-V1, Biogelx and KLD because YIGSR in KLD-V2 promoted cell population and ECM secretion by the interaction with cells and increased vasculogenesis. Overall, the designed SAP hydrogel represents an effective scaffold for vascularization of tissue constructs with useful tissue engineering applications. © The Royal Society of Chemistry 2021.
Açıklama
Anahtar Kelimeler
Atomic force microscopy, Biomimetics, Cell adhesion, Cell culture, Cell proliferation, Endothelial cells, Gene expression, Glycoproteins, Histology, Hydrogels, Nanofibers, Peptides, Polymerase chain reaction, Scanning electron microscopy, Stem cells, Tissue, Cell adhesion molecules, Extracellular matrices, Human umbilical endothelial cells (HUVECs), Molecular integrations, Quantitative polymerase chain reaction, Scanning electron microscopy image, Self-assembled peptides, Tissue engineering applications, Scaffolds (biology), peptide, cell differentiation, human, hydrogel, mesenchymal stem cell, tissue engineering, tissue scaffold, Cell Differentiation, Humans, Hydrogels, Mesenchymal Stem Cells, Peptides, Tissue Engineering, Tissue Scaffolds
Kaynak
Soft Matter
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
17
Sayı
27
