Genotypic analysis of Escherichia coli strains that cause urosepsis in the Aegean region

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dc.contributor.authorGiray, Betul
dc.contributor.authorUcar, Fusun Bahriye
dc.contributor.authorAydemir, Sabire Sohret
dc.date.accessioned2019-10-27T22:57:45Z
dc.date.available2019-10-27T22:57:45Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground/aim: The aim of this study was to characterize strains genotypically, to determine their phylogenetic relationships, to investigate the presence of the papG gene, and to compare their antibiotic susceptibility test results. Materials and methods: Seventy pathogenic E. coli strains were isolated from both urine and blood cultures of patients with the preliminary diagnosis of urosepsis who were referred to the Ege University Faculty of Medicine, Bacteriology Laboratory of Medical Microbiology Department in Izmir. All of these strains were examined for the papG gene and phylogenetic groups with the multiplex polymerase chain reaction technique. Pulsed-field gel electrophoresis and multilocus sequence typing (MLST) were used for epidemiologic analysis. Results: Phylogenetically, it was found that 16 belonged to group B2, 31 belonged to group D, 15 belonged to group A, and 7 belonged to group B1. One strain was not identified as belonging to a group. papG genes were found in 26 of 70 E. coli strains. Thirty urosepsis pathogenic E. coli strains were analyzed with MLST. Twenty-two strains were identified as new STs. Conclusion: These findings are extremely important for Turkey and these new 22 strains should be investigated in more detail because they are new and have the potential to lead to infections.en_US
dc.description.sponsorshipEge University Scientific Research Project (BAP)Ege University [2013-FEN-009]en_US
dc.description.sponsorshipThis study was supported by the Ege University Scientific Research Project (BAP), 2013-FEN-009. We would like to extend our thanks to Prof Dr James R Johnson, who provided us with type species, and to Mert Sudagidan, who helped us with PFGE analysis. The molecular research was conducted at the Institute of High Technology in Izmir.en_US
dc.identifier.doi10.3906/sag-1507-114
dc.identifier.endpage1527en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue5en_US
dc.identifier.pmid27966323en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1518en_US
dc.identifier.urihttps://doi.org/10.3906/sag-1507-114
dc.identifier.urihttps://hdl.handle.net/11454/51314
dc.identifier.volume46en_US
dc.identifier.wosWOS:000389053000037en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectE. colien_US
dc.subjectpapGen_US
dc.subjecturosepsisen_US
dc.subjectphylogenetic groupingen_US
dc.subjectPFGEen_US
dc.titleGenotypic analysis of Escherichia coli strains that cause urosepsis in the Aegean regionen_US
dc.typeArticleen_US

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