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Öğe Design and Development of a Novel Electrochemical DNA Biosensor for Rapid Molecular Identification of Enterococcus faecium(Elsevier Sci Ltd, 2008) Chan, Y. Y.; Balqis, K.; Dilsat, A. O.; Lee, S. Y.; Lalitha, P.; Ozsoz, M.; Ravichandran, M.Öğe Effect of Surface Chemistry Method in Electrochemical Genosensing for Optimum Detection of Pathogens(Electrochemical Soc Inc, 2016) Kara, P.; Yilmaz, O.; Kilickaya, O.; Ozsoz, M.Herein we describe the effect of surface chemistry method for rapid, label free and direct electrochemical detection of microbiological diseases in a long amplicon sequence. Electrochemical Impedance Spectrometry (EIS) was used as signal transduction. E. coli amplicon was used as model case. Hybridization detection was performed by using several surface chemistry methods including, adsorbtion, carbodiimide chemistry, self-assemble monolayer (SAM), polypyrrole polymerization, polylysine coating, silane mediated and gold nanoparticles (AuNP) at disposable graphite (DGE) and gold (AuE) electrode surfaces. Designed genosensors' selectivities and the detection limits were compared to determine optimum pathogen detection. AuNP modified surface was chosen as optimum sensor at the detection limit of E. coli amplicon was 160.3 pM and sensor sensitivity based on Full Match/Non Complementary (FM/NC) ratio of 6.3. (C) 2016 The Electrochemical Society. All rights reserved.Öğe Electrochemical aptasensor design for label free cytosensing of human non-small cell lung cancer(Elsevier Science Sa, 2016) Kara, P.; Erzurumlu, Y.; Kirmizibayrak, P. Ballar; Ozsoz, M.A roboust aptamer based electrochemical cytosensing platform for detection of adenocarcinoma type lung cancer based on electrochemical impedance spectrometry (EIS) was developed with high sensitivity, selectivity and devoted to medical applications. Screen printed carbon electrodes (SCPEs) were used as sensor surface, 5'amino linked aptamer sequence was immobilized onto the SPCEs, interaction with cancer cells at 37.5 degrees C was monitored by EIS transduction of the Rct in the presence of 5 mM [Fe(CN)(6)](3-/4-). Designed aptasensor's selectivity was determined by using Human liver hepatocellular carcinoma (HepG2) and human cervical cancer (HeLA) cells. The purposed cytosensor showed high sensitivity with a detection limit of 163.7 cells/mL. This simple, rapid and low cost electrochemical approach offered a label free detection with capable of early detection in cancer diagnosis with interest for future applications. (C) 2016 Published by Elsevier B.V.Öğe Electrochemical DNA Biosensors for Detection of Compound-DNA Interactions(Pan Stanford Publishing Pte Ltd, 2012) Ozkan-Ariksoysal, D.; Kara, P.; Ozsoz, M.; Ozsoz, MÖğe Electrochemical Nucleic Acid Biosensors Based on Hybridization Detection for Clinical Analysis(Pan Stanford Publishing Pte Ltd, 2012) Kara, P.; Ariksoysal, D.; Ozsoz, M.; Ozsoz, MÖğe Response Surface Methodology for Optimization of Food Borne Pathogen Detection in Real Samples Based on Label Free Electrochemical Nucleic Acid Biosensors(Wiley-Blackwell, 2011) Urkut, Z.; Kara, P.; Goksungur, Y.; Ozsoz, M.The response surface methodology (RSM) is used for optimization of foodborne pathogen detection based on label free electrochemical nucleic acid biosensors. Listeria monocytogenes amplicons obtained from food samples are used as model case. The extent of hybridization is determined by using guanine oxidation signals obtained with Differential Pulse Voltammetry and Electrochemical Impedance Spectroscopy. RSM is used to investigate the effects of hybridization parameters including target and salt concentration and hybridization time on the biosensor selectivity. The ratio of electrochemical transductions after hybridization with complementary and noncomplementary targets is the response of the statistical analysis obtaining a detection limit of 267 pM.
