Effect of Surface Chemistry Method in Electrochemical Genosensing for Optimum Detection of Pathogens
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Electrochemical Soc Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Herein we describe the effect of surface chemistry method for rapid, label free and direct electrochemical detection of microbiological diseases in a long amplicon sequence. Electrochemical Impedance Spectrometry (EIS) was used as signal transduction. E. coli amplicon was used as model case. Hybridization detection was performed by using several surface chemistry methods including, adsorbtion, carbodiimide chemistry, self-assemble monolayer (SAM), polypyrrole polymerization, polylysine coating, silane mediated and gold nanoparticles (AuNP) at disposable graphite (DGE) and gold (AuE) electrode surfaces. Designed genosensors' selectivities and the detection limits were compared to determine optimum pathogen detection. AuNP modified surface was chosen as optimum sensor at the detection limit of E. coli amplicon was 160.3 pM and sensor sensitivity based on Full Match/Non Complementary (FM/NC) ratio of 6.3. (C) 2016 The Electrochemical Society. All rights reserved.
Açıklama
Anahtar Kelimeler
Kaynak
Journal of the Electrochemical Society
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
163
Sayı
7
