Response Surface Methodology for Optimization of Food Borne Pathogen Detection in Real Samples Based on Label Free Electrochemical Nucleic Acid Biosensors
Küçük Resim Yok
Tarih
2011
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-Blackwell
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The response surface methodology (RSM) is used for optimization of foodborne pathogen detection based on label free electrochemical nucleic acid biosensors. Listeria monocytogenes amplicons obtained from food samples are used as model case. The extent of hybridization is determined by using guanine oxidation signals obtained with Differential Pulse Voltammetry and Electrochemical Impedance Spectroscopy. RSM is used to investigate the effects of hybridization parameters including target and salt concentration and hybridization time on the biosensor selectivity. The ratio of electrochemical transductions after hybridization with complementary and noncomplementary targets is the response of the statistical analysis obtaining a detection limit of 267 pM.
Açıklama
Anahtar Kelimeler
Nucleic acid biosensor, Voltammetry, Electrochemical impedance spectroscopy (EIS), Listeria monocytogenes, Response surface methodology (RSM)
Kaynak
Electroanalysis
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
23
Sayı
11
