Yazar "Ghorbanizamani, Faezeh" seçeneğine göre listele
Listeleniyor 1 - 20 / 35
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe A combinatorial approach to chicken meat spoilage detection using color-shifting silver nanoparticles, smartphone imaging, and artificial neural network (ANN)(Elsevier Sci Ltd, 2024) Ghorbanizamani, FaezehEnsuring food freshness is crucial for public health. Biogenic amines (like histamine) are reliable spoilage indicators in protein-rich foods such as meat. This study presents a label-free colorimetric sensor using green- colored silver nanoparticles (AgNPs) functionalized with carboxylated polyvinylpyrrolidone (PVP-COOH) for sensitive BA detection. After optimizing pH, time, and temperature, the modified AgNPs achieved a detection limit (LOD) of 0.21 mu g/mL and an analytical dynamic range of 10-100 mu g/mL for histamine. Smartphone imaging was employed to capture colorimetric changes, and the extracted data were used to train an artificial neural network (ANN), enhancing the LOD to 0.09 mu g/mL and extending the dynamic range to 0.5-200 mu g/mL. The sensor was validated with real food samples, successfully monitoring histamine levels in chicken meat over three days, detecting spoilage-related changes with high sensitivity. This integrative approach combining AgNPs, smartphone imaging, and AI offers a powerful tool for advanced food freshness monitoring.Öğe Artemisia alleviates AGE-induced liver complications via MAPK and RAGE signaling pathways modulation: a combinatorial study(Springer, 2022) Moulahoum, Hichem; Ghorbanizamani, Faezeh; Khiari, Zineb; Toumi, Mohamed; Benazzoug, Yasmina; Tok, Kerem; Timur, SunaArtemisia herba-alba (AHA) is a traditionally used plant to treat various diseases, including diabetes and metabolic dysfunctions. Plant extracts are generally explored empirically without a deeper assessment of their mechanism of action. Here, we describe a combinatorial study of biochemical, molecular, and bioinformatic (metabolite-protein pharmacology network) analyses to elucidate the mechanism of action of AHA and shed light on its multilevel effects in the treatment of diabetes-related advanced glycation end-products (AGE)-induced liver damages. The extract's polyphenols and flavonoids content were measured and then identified via LC-Q-TOF-MS/MS. Active compounds were used to generate a metabolite-target interaction network via Swiss Target Prediction and other databases. The extract was tested for its antiglycation and aggregation properties. Next, THLE-2 liver cells were challenged with AGEs, and the mechanistic markers were measured [TNF-alpha, IL-6, nitric oxide, total antioxidant capacity, lipid peroxidation (LPO), and caspase 3]. Metabolite and network screening showed the involvement of AHA in diabetes, glycation, liver diseases, aging, and apoptosis. Experimental confirmation showed that AHA inhibited protein modification and AGE formation. Additionally, AHA reduced inflammatory mediators (IL-6, TNF alpha), oxidative stress markers (NO, LPO), and apoptosis (Caspase 3). On the other hand, cellular total antioxidant capacity was restored to normal levels. The combinatorial study showed that AHA regulates AGE-induced liver damages through MAPK-AKT and AGE-RAGE signaling pathways. This report highlights the combination of experimental and network pharmacology for the exact elucidation of AHA mechanism of action as a multitarget option in the therapy of diabetes and AGEs-related diseases.Öğe Carbon dots and curcumin-loaded CD44-Targeted liposomes for imaging and tracking cancer chemotherapy: A multi-purpose tool for theranostics(Elsevier, 2021) Demir, Bilal; Moulahoum, Hichem; Ghorbanizamani, Faezeh; Barlas, F. Baris; Yesiltepe, Ozan; Gumus, Z. Pinar; Timur, SunaGrowing needs in biomedical applications have driven the development of multifunctional nanoparticles towards theranostically-engineered multimodal platforms. Many strategies for point-effective cellular uptake of theranostics have been described. in this report, multifunctional nanostructures were designed and synthesized as a novel theranostic platform. Liposomes were loaded with curcumin as a therapeutic agent and carbon dots (CDs) as a contrast molecule. Anti-CD44 antibodies were bioconjugated on the liposomal surface to obtain an active targeting nanocarrier. After synthesis, the relative characterization of the vesicles was performed using DLS and TEM techniques. in vitro analysis was conducted to investigate the multimodal properties of the theranostic via testing its toxicity, cell uptake, bioimaging, and chemo/radiotherapy applications on two different cell lines. 3D holographic microscopy was employed to track the treatment effects during experimentation. Results indicated that these particles are efficient tools for clinical applications due to their fluorescent characteristic and increased bioperformance.Öğe Combination of LC-Q-TOF-MS/MS, network pharmacology, and nanoemulsion approaches identifies active compounds of two Artemisia species responsible for tackling early diabetes-related metabolic complications in the liver(Wiley, 2022) Moulahoum, Hichem; Ghorbanizamani, Faezeh; Khiari, Zineb; Toumi, Mohamed; Benazzoug, Yasmina; Timur, Suna; Zihnioglu, FigenIntroduction The chronicity of advanced glycation end-products (AGEs) imparts various damages resulting in metabolic dysfunction and diseases involving inflammation and oxidative stress. The use of plant extracts is of high interest in complementary medicine. Yet, extracts are multicomponent mixtures, and difficult to pinpoint their exact mechanism. Objectives We hypothesise that network pharmacology and bioinformatics can help experimental findings depict the exact active components and mechanism of action by which they induce their effects. Additionally, the toxicity and variability can be lowered and standardised with proper encapsulation methods. Methodology Here, we propose the formulation of phytoniosomes encapsulating two Artemisia species (Artemisia dracunculus and Artemisia absinthium) to mitigate AGEs and their induced cell redox dysregulation in the liver. Extracts from different solvents were identified via liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS/MS). Phytoniosomes were explored for their anti-glycating effect and modulation of AGE-induced damages in THLE-2 liver cells. Network pharmacology tools were used to identify possible targets and signalling pathways implicated. Results Data demonstrated that A. absinthium phytoniosomes had a significant anti-AGE effect comparable to reference molecules and higher than A. dracunculus. They were able to restore cell dysfunction through the restoration of tumour necrosis alpha (TNF-alpha), interleukin 6 (IL-6), nitric oxide, and total antioxidant capacity. Phytoniosomes were able to protect cells from apoptosis by decreasing caspase 3 activity. Network pharmacology and bioinformatic analysis confirmed the induction of the effect via Akt-PI3K-MAPK and AGE-RAGE signalling pathways through quercetin and luteolin actions. Conclusion The current report highlights the potential of Artemisia phytoniosomes as strong contenders in AGE-related disease therapy.Öğe Design of Polymeric Surfaces as Platforms for Streamlined Cancer Diagnostics in Liquid Biopsies(MDPI, 2023) Ghorbanizamani, Faezeh; Moulahoum, Hichem; Celik, Emine Guler; Zihnioglu, Figen; Beduk, Tutku; Goksel, Tuncay; Turhan, KutsalMinimally invasive approaches for cancer diagnosis are an integral step in the quest to improve cancer survival. Liquid biopsies such as blood samples are matrices explored to extract valuable information about the tumor and its state through various indicators, such as proteins, peptides, tumor DNA, or circulating tumor cells. Although these markers are scarce, making their isolation and detection in complex matrices challenging, the development in polymer chemistry producing interesting structures, including molecularly imprinted polymers, branched polymers, nanopolymer composites, and hybrids, allowed the development of enhanced platforms with impressive performance for liquid biopsies analysis. This review describes the latest advances and developments in polymer synthesis and their application for minimally invasive cancer diagnosis. The polymer structures improve the operational performances of biosensors through various processes, such as increased affinity for enhanced sensitivity, improved binding, and avoidance of non-specific interactions for enhanced specificity. Furthermore, polymer-based materials can be a tremendous help in signal amplification of usually low-concentrated targets in the sample. The pros and cons of these materials, how the synthesis process affects their performance, and the device applications for liquid biopsies diagnosis will be critically reviewed to show the essentiality of this technology in oncology and clinical biomedicine.Öğe Dye-Loaded Polymersome-Based Lateral Flow Assay: Rational Design of a COVID-19 Testing Platform by Repurposing SARS-CoV-2 Antibody Cocktail and Antigens Obtained from Positive Human Samples(Amer Chemical Soc, 2021) Ghorbanizamani, Faezeh; Tok, Kerem; Moulahoum, Hichem; Harmanci, Duygu; Hanoglu, Simge Balaban; Durmus, Ceren; Zihnioglu, FigenThe global pandemic of COVID-19 continues to be an important threat, especially with the fast transmission rate observed after the discovery of novel mutations. In this perspective, prompt diagnosis requires massive economical and human resources to mitigate the disease. The current study proposes a rational design of a colorimetric lateral flow immunoassay (LFA) based on the repurposing of human samples to produce COVID-19-specific antigens and antibodies in combination with a novel dye-loaded polymersome for naked-eye detection. A group of 121 human samples (61 serums and 60 nasal swabs) were obtained and analyzed by RT-PCR and ELISA. Pooled samples were used to purify antibodies using affinity chromatography, while antigens were purified via magnetic nanoparticles-based affinity. The purified proteins were confirmed for their specificity to COVID-19 via commercial LFA, ELISA, and electrochemical tests in addition to sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Polymersomes were prepared using methoxy polyethylene glycol-b-polycaprolactone (mPEG-b-PCL) diblock copolymers and loaded with a Coomassie Blue dye. The polymersomes were then functionalized with the purified antibodies and applied for the preparation of two types of LFA (antigen test and antibody test). Overall, the proposed diagnostic tests demonstrated 93 and 92.2% sensitivity for antigen and antibody tests, respectively. The repeatability (92-94%) and reproducibility (96-98%) of the tests highlight the potential of the proposed LFA. The LFA test was also analyzed for stability, and after 4 weeks, 91-97% correct diagnosis was observed. The current LFA platform is a valuable assay that has great economical and analytical potential for widespread applications.Öğe Emerging trends in nanomaterial design for the development of point-of-care platforms and practical applications(Elsevier, 2023) Moulahoum, Hichem; Ghorbanizamani, Faezeh; Beduk, Tutku; Bedük, Duygu; Özufuklar, Özge; Güler Çelik, Emine; Timur, SunaNanomaterials and nanotechnology offer promising opportunities in point-of-care (POC) diagnostics and therapeutics due to their unique physical and chemical properties. POC platforms aim to provide rapid and portable diagnostic and therapeutic capabilities at the site of patient care, offering cost-effective solutions. Incorporating nanomaterials with distinct optical, electrical, and magnetic properties can revolutionize the POC industry, significantly enhancing the effectiveness and efficiency of diagnostic and theragnostic devices. By leveraging nanoparticles and nanofibers in POC devices, nanomaterials have the potential to improve the accuracy and speed of diagnostic tests, making them more practical for POC settings. Technological advancements, such as smartphone integration, imagery instruments, and attachments, complement and expand the application scope of POCs, reducing invasiveness by enabling analysis of various matrices like saliva and breath. These integrated testing platforms facilitate procedures without compromising diagnosis quality. This review provides a summary of recent trends in POC technologies utilizing nanomaterials and nanotechnologies for analyzing disease biomarkers. It highlights advances in device development, nanomaterial design, and their applications in POC. Additionally, complementary tools used in POC and nanomaterials are discussed, followed by critical analysis of challenges and future directions for these technologies.Öğe Fluorescent bioassay for SARS-CoV-2 detection using polypyrene-g-poly(epsilon-caprolactone) prepared by simultaneous photoinduced step-growth and ring-opening polymerizations(Springer Wien, 2022) Celiker, Tugba; Ghorbanizamani, Faezeh; Moulahoum, Hichem; Guler Celik, Emine; Tok, Kerem; Zihnioglu, Figen; Cicek, CandanThe construction of a rapid and easy immunofluorescence bioassay for SARS-CoV-2 detection is described. We report for the first time a novel one-pot synthetic approach for simultaneous photoinduced step-growth polymerization of pyrene (Py) and ring-opening polymerization of epsilon-caprolactone (PCL) to produce a graft fluorescent copolymer PPy-g-PCL that was conjugated to SARS-CoV-2-specific antibodies using EDC/NHS chemistry. The synthesis steps and conjugation products were fully characterized using standard spectral analysis. Next, the PPy-g-PCL was used for the construction of a dot-blot assay which was calibrated for applications to human nasopharyngeal samples. The analytical features of the proposed sensor showed a detection range of 6.03-8.7 LOG viral copy mL(-1) (Ct Scores: 8-25), the limit of detection (LOD), and quantification (LOQ) of 1.84 and 6.16 LOG viral copy mL(-1), respectively. The repeatability and reproducibility of the platform had a coefficient of variation (CV) ranging between 1.2 and 5.9%. The fluorescence-based dot-blot assay was tested with human samples. Significant differences were observed between the fluorescence intensity of the negative and positive samples, with an overall correct response of 93.33%. The assay demonstrated a high correlation with RT-PCR data. This strategy opens new insights into simplified synthesis procedures of the reporter molecules and their high potential sensing and diagnosis applications.Öğe Glutathione Encapsulation in Core-Shell Drug Nanocarriers (Polymersomes and Niosomes) Prevents Advanced Glycation End-products Toxicities(Springer, 2021) Ghorbanizamani, Faezeh; Moulahoum, Hichem; Bayir, Ece; Zihnioglu, Figen; Timur, SunaThe clinical application of some natural molecules in therapy is usually limited due to the lack of feasible delivery systems. Core-shell nanocarriers (polymersomes and niosomes) are interesting stable nanocarriers that are biocompatible, biodegradable, and able to produce sustainable delivery. They can be modified and functionalized according to the application needed. In this report, we describe the synthesis of a smart and pH-responsive poly(ethylene oxide)-poly(lactide) polymersome (PEO-PL) and niosomes (NIO) loaded with GSH for efficient peptide delivery and potent application against advanced glycation end-products-related damages and toxicities. PEO-PL was constructed using the one-pot sequential anionic ring-opening polymerization method, while the niosomes were prepared via the thin film layer technique. The nanocarriers were characterized for their size and morphology by DLS and SEM. The formulations demonstrated a high encapsulation rate reaching 95% and showed increased sensitivity to release their content in acidic conditions (pH 5.5) compared with physiological media. PEO-PL showed a higher retention rate compared with the NIO. The nanocarriers showed no apparent toxicity even at high concentrations (400 mu g/mL). The MTT test demonstrated that HeLa cell lines were more sensitive to GSH than U87 cell lines starting from 100 and 400 mu g/mL, respectively. Testing the synthesized core-shell nanocarriers on altered proteins showed prolonged and high prevention rates of glycation, aggregation, and oxidation without hindering the effect of GSH. These bioresponsive nanocarriers appear as an interesting platform for biomedicine and therapy.Öğe How should diagnostic kits development adapt quickly in COVID 19-like pandemic models? Pros and cons of sensory platforms used in COVID-19 sensing(Elsevier, 2021) Moulahoum, Hichem; Ghorbanizamani, Faezeh; Zihnioglu, Figen; Turhan, Kutsal; Timur, SunaAs COVID-19 has reached pandemic status and the number of cases continues to grow, widespread availability of diagnostic testing is critical in helping identify and control the emergence of this rapidly spreading and serious illness. However, a lacking in making a quick reaction to the threat and starting early development of diagnostic sensing tools has had an important impact globally. in this regard, here we will review critically the current developed diagnostic tools in response to the COVID-19 pandemic and compare the different types through the discussion of their pros and cons such as nucleic acid detection tests (including PCR and CRISPR), antibody and protein-based diagnosis tests. in addition, potential technologies that are under development such as on-site diagnosis platforms, lateral flow, and portable PCR units are discussed. Data collection and epidemiological analysis could also be an interesting factor to incorporate with the emerging technologies especially with the wide access to smartphones. Lastly, a SWOT analysis and perspectives on how the development of novel sensory platforms should be treated by the different decision-makers are analyzed.Öğe Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing(Elsevier, 2022) Durmus, Ceren; Harmanci, Duygu; Moulahoum, Hichem; Tok, Kerem; Ghorbanizamani, Faezeh; Sanli, Serdar; Zihnioglu, FigenThe increasing mutation frequency of the SARS-CoV-2 virus and the emergence of successive variants have made correct diagnosis hard to perform. Developing efficient and accurate methods to diagnose infected patients is crucial to effectively mitigate the pandemic. Here, we developed an electrochemical immunosensor based on SARS-CoV-2 antibody cocktail-conjugated magnetic nanoparticles for the sensitive and accurate detection of the SARS-CoV-2 virus and its variants in nasopharyngeal swabs. The application of the antibody cocktail was compared with commercially available anti-SARS-CoV-2 S1 (anti-S1) and anti-S2 monoclonal antibodies. After optimization and calibration, the limit of detection (LOD) determination demonstrated a LOD = 0.53-0.75 ng/ mL for the antibody cocktail-based sensor compared with 0.93 ng/mL and 0.99 ng/mL for the platforms using anti-S1 and anti-S2, respectively. The platforms were tested with human nasopharyngeal swab samples pre diagnosed with RT-PCR (10 negatives and 40 positive samples). The positive samples include the original, alpha, beta, and delta variants (n = 10, for each). The polyclonal antibody cocktail performed better than commercial anti-S1 and anti-S2 antibodies for all samples reaching 100% overall sensitivity, specificity, and accuracy. It also showed a wide range of variants detection compared to monoclonal antibody-based platforms. The present work proposes a versatile electrochemical biosensor for the indiscriminate detection of the different variants of SARS-CoV-2 using a polyclonal antibody cocktail. Such diagnostic tools allowing the detection of variants can be of great efficiency and economic value in the fight against the ever-changing SARS-CoV-2 virus.Öğe Ionic liquid-hydrogel hybrid material for enhanced electron transfer and sensitivity towards electrochemical detection of methamphetamine(Elsevier, 2022) Ghorbanizamani, Faezeh; Moulahoum, Hichem; Celik, Emine Guler; Timur, SunaReliable onsite biosensors for detecting drugs of abuse are of critical importance for the current society. Electrochemical sensors, especially screen-printed electrode (SPE)-based sensors, are a strong candidate for onsite drug detection. Although many SPE-based sensors are being developed, enhancing these tools' sensitivity and analytical capacity is a continuous endeavor. Here, we propose a novel approach for SPE surface modification using a combination of hydrogel, ionic liquid, and specific antibodies to detect methamphetamine in human saliva samples. The hydrogel-ionic liquid (ionogel) was synthesized from gelatin, PEG, and 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide. FTIR and SEM were used to characterize the material before it was applied over a screen-printed gold electrode for electrochemical measurements using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). After optimization, the calibration of the proposed sensor showed a detection range between 5.0 and 1000 ng/mL with a LOD = 0.56 ng/mL in PBS. Application of the sensor with spiked human saliva demonstrated enhanced sensitivity with a LOD = 0.72 ng/mL. The repeatability and reproducibility of the proposed biosensor demonstrated a coefficient of variance (CV%) reaching 6.92% and 2.53%, respectively. The biosensor showed a high specificity toward methamphetamine compared to other interferents. The current ionogel-based electrochemical immunosensor offers a promising and versatile tool for sensitive onsite detection of substance abuse. (C) 2022 Elsevier B.V. All rights reserved.Öğe Ionic liquid-reinforced Hydroxyapatite@nano-TiO2 as a green platform for Immuno-electrochemical sensing applications(Elsevier, 2024) Ghorbanizamani, Faezeh; Moulahoum, Hichem; Timur, SunaIn contemporary society, developing dependable point-of-care (POC) biosensors for the timely detection of cancer markers is crucial. Among various sensor types, screen-printed electrode (SPE)-based sensors, particularly electrochemical ones, stand out as promising candidates for POC applications. Despite ongoing efforts to create numerous SPE-based sensors, there is a continuous pursuit to enhance their sensitivity and analytical capabilities. This study presents an advanced electrochemical sensor designed to sensitively detect the hepatocellular carcinoma (HCC) marker Alpha-fetoprotein (AFP) in saliva. The sensor employs a gold SPE modified with hydroxyapatite, TiO2 2 nanoparticles, 1-butyl-3-methylimidazolium bis(trifluoromethyl sulfonyl)imide ionic liquid (IL), and AFP monoclonal antibodies. After thorough characterization and optimization using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), the biosensor exhibited a broad detection range (0.01-400 ng/ mL), a low limit of detection (LOD) at 0.058 ng/mL, and demonstrated high selectivity, repeatability, reproducibility, and stability. Furthermore, when tested with spiked human saliva samples, the biosensor displayed excellent recovery and robustness, showcasing its potential for noninvasive and POC diagnosis of HCC. In an environmentally conscious evaluation, the biosensor's greenness was assessed using the AGREE metric, yielding a high score of 0.85. This score indicates the biosensor's alignment with the principles of green analytical chemistry, underlining its eco-friendly attributes. This innovative electrochemical sensor contributes to the ongoing efforts for efficient and reliable POC diagnostic tools and aligns with a broader commitment to developing environmentally friendly solutions.Öğe Ionic liquids enhancement of hydrogels and impact on biosensing applications(Elsevier, 2022) Ghorbanizamani, Faezeh; Moulahoum, Hichem; Celik, Emine Guler; Timur, SunaThe pursuit of novel materials to enhance specific features and the analytical efficiency of sensors is a hot topic in the biomaterial engineering field. Hydrogels are an important material category that has a good value in biosensing applications due to their immobilization and protection capabilities that enhance their biocompatibility. Progress in development allowed the production of intelligent hydrogel materials that can function as a stimuli-responsive medium in sensing signal actuation. A novel concept in hydrogel-enabled biosensing development is the addition of ionic liquids (ILs) to create unique structures called ionic gels that possess both the intrinsic features of hydrogels and ILs. The current review is a straightforward overview of the two materials (hydrogels and ion gels) with a strong interest in their potential in biosensing applications. A highlight of the most recent and impactful research is analyzed to provide a solid standing reference point for future development.(c) 2022 Elsevier B.V. All rights reserved.Öğe Ionic Liquids from Biocompatibility and Electrochemical Aspects toward Applying in Biosensing Devices(Amer Chemical Soc, 2018) Ghorbanizamani, Faezeh; Timur, SunaThe introduction of a novel ionic environment, which is composed of a large, asymmetric organic cation and inorganic (or organic) anion that loosely fit together, is extending the properties and classical applications of chemical/biochemical and industrial performances. In this Feature, we discuss the recent uses of ionic liquids in enzyme activation and their combination with nanosized materials and electrode structures to enhance the sensing performance of biobased sensing devices.Öğe Laser-printed paper ELISA and hydroxyapatite immobilization for colorimetric congenital anomalies screening in saliva(Elsevier, 2024) Moulahoum, Hichem; Ghorbanizamani, Faezeh; Timur, SunaBackground: Alpha-fetoprotein (AFP) is a fetal protein that can indicate congenital anomalies such as Down syndrome and spinal canal blockage when detected at abnormal levels in pregnant women. Current AFP detection methods rely on invasive blood or serum samples, which require sophisticated equipment. From the many solutions proposed, colorimetric paper -based assays excel in point -of -care settings. The concept of paperbased ELISA (p-ELISA) enhances traditional methods, aligning with the ASSURED criteria for diagnostics in resource -limited regions. Despite success in microfluidic paper -based assay devices, laser printing remains underexplored for p-ELISA. Additionally, modifying the paper surface provides an additional layer of sensitivity enhancement. Results: In this study, we developed a novel laser -printed paper -based ELISA (LP-pELISA) for rapid, sensitive, and noninvasive detection of AFP in saliva samples. The LP-pELISA platform was fabricated by printing hydrophobic barriers on filter paper using a laser printer, followed by depositing hydroxyapatite (HAp) as an immobilization material for the antibodies. The colorimetric detection was achieved using AuNPs functionalized with anti-AFP antibodies and silver nitrate enhancement. The LP-pELISA exhibited a linear response for AFP detection in both buffer and saliva samples over a range of 1.0-800 ng mL -1 , with a limit of detection (LOD) reaching 1.0 ng mL -1 . The assay also demonstrated good selectivity, repeatability, reproducibility, and stability. The LP-pELISA was further validated by testing spiked human saliva samples, showing its potential for point -of -care diagnosis of congenital disabilities. Significance: The LP-pELISA is a noninvasive platform showcasing simplicity, cost-effectiveness, and userfriendliness, utilizing laser printing, hydroxyapatite modification, and saliva samples to efficiently detect AFP. Beyond its application for AFP, this method's versatility extends to other biomarkers, positioning it as a catalyst for the evolution of paper -based biosensors. The LP-pELISA holds promise as a transformative tool for point -ofcare diagnostics, fostering advancements in healthcare with its innovative technology.Öğe Lightless catalytic layered chitosan coating film using doped TiO2@metal ions nanoparticles for highly efficient dye degradation in aqueous media and disinfection applications(Elsevier Science Sa, 2022) Moulahoum, Hichem; Ghorbanizamani, Faezeh; Sakarya, Serhan; Timur, SunaChitosan-based materials are widely used in various industries due to their environmental friendliness (biodegradable and non-toxicity) and other antimicrobial properties. Improving the chitosan capacities using additional components such as titanium dioxide (TiO2) and other nanoparticles proves beneficial. Here, we propose the combination of chitosan-TiO2 nanocomposites (ChiTiO) with various metal ions (silver, zinc, copper, and iron), forming novel chitosan-based films to improve the capacities of the material further. The ChiTiO-ion metals nanocomposites and films were synthesized and characterized using dynamic light scattering (DLS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), ultraviolet-visible spectroscopy (UV-Vis), Fourier transforms infrared spectroscopy (FT-IR), X-ray fluorescence (XRF), and cyclic voltammetry techniques. The biological activities of the metal-doped formulations showed increased antioxidant activities led by ChiTiO@Ag. Additionally, the methylene blue (MB) dye degradation capabilities were dominated by ChiTiO@Cu. The material showed a higher specificity towards MB than other dyes (methyl orange and bromophenol blue). The photocatalytic degradation at high temperatures (60 degrees C) was faster than at ambient (25 degrees C) and cold (4 degrees C) temperatures. The reusability of the film showed sustained photocatalytic capacities even after three cycles with a low loss of 16.7%. The antimicrobial activities of the films were tested against three pathogens (S. aureus, C. albicans, and P. aeruginosa) under different light conditions. Data showed ChiTiO@Ag with a concentration of 0.5 g composite having the best activity compared with the other materials. The most important finding is the ability of the proposed films to perform their activities without the need for any light activation. Lastly, ChiTiO@ion metals provide promising applications as ambient light packaging materials, coating materials, and photocatalysts.Öğe Material Design in Implantable Biosensors toward Future Personalized Diagnostics and Treatments(MDPI, 2023) Ghorbanizamani, Faezeh; Moulahoum, Hichem; Celik, Emine Guler; Timur, SunaThe growing demand for personalized treatments and the constant observation of vital signs for extended periods could positively solve the problematic concerns associated with the necessity for patient control and hospitalization. The impressive development in biosensing devices has led to the creation of man-made implantable devices that are temporarily or permanently introduced into the human body, and thus, diminishing the pain and discomfort of the person. Despite all promising achievements in this field, there are some critical challenges to preserve reliable functionality in the complex environment of the human body over time. Biosensors in the in vivo environment are required to have specific features, including biocompatibility (minimal immune response or biofouling), biodegradability, reliability, high accuracy, and miniaturization (flexible, stretchable, lightweight, and ultra-thin). However, the performance of implantable biosensors is limited by body responses and insufficient power supplies (due to minimized batteries/electronics and data transmission without wires). In addition, the current processes and developments in the implantable biosensors field will open new routes in biomedicine and diagnostic systems that monitor occurrences happening inside the body in a certain period. This topical paper aims to give an overview of the state-of-the-art implantable biosensors and their design methods. It also discusses the latest developments in material science, including nanomaterials, hydrogel, hydrophilic, biomimetic, and other polymeric materials to overcome failures in implantable biosensors' reliability. Lastly, we discuss the main challenges faced and future research prospects toward the development of dependable implantable biosensors.Öğe Molecularly imprinted polymers-based biosensors for gynecological diagnostics and monitoring(Elsevier, 2024) Ghorbanizamani, Faezeh; Moulahoum, Hichem; Zihnioglu, Figen; Timur, SunaMolecularly Imprinted Polymers (MIPs) offer promising advancements in gynecological diagnostics due to their high selectivity, stability, and cost-effectiveness. This review explores the application of MIP-based biosensors in detecting biomarkers for gynecological cancer, infections, and hormonal monitoring. Despite significant progress in MIP technology, its integration into clinical gynecology remains limited. The review provides a deep dive into the synthesis and characterization process of MIPs, current diagnostic methods, and the potential of emerging diagnostic approaches such as microfluidics and nanotechnology. Then, an overview of the various conditions, diseases, and potential biomarkers is explored. Emphasizing the importance of women's health, the review analyzes the latest research in MIP-based biosensing of gynecological conditions and calls for increased research and development to bridge the gap between laboratory innovation and clinical application. The goal is to enhance early detection, improve patient outcomes, and reduce healthcare costs. This advancement is essential for better disease management and personalized treatment in gynecology.Öğe Nano-Scaled Materials and Polymer Integration in Biosensing Tools(Mdpi, 2022) Moulahoum, Hichem; Ghorbanizamani, Faezeh; Guler Celik, Emine; Timur, SunaThe evolution of biosensors and diagnostic devices has been thriving in its ability to provide reliable tools with simplified operation steps. These evolutions have paved the way for further advances in sensing materials, strategies, and device structures. Polymeric composite materials can be formed into nanostructures and networks of different types, including hydrogels, vesicles, dendrimers, molecularly imprinted polymers (MIP), etc. Due to their biocompatibility, flexibility, and low prices, they are promising tools for future lab-on-chip devices as both manufacturing materials and immobilization surfaces. Polymers can also allow the construction of scaffold materials and 3D structures that further elevate the sensing capabilities of traditional 2D biosensors. This review discusses the latest developments in nano-scaled materials and synthesis techniques for polymer structures and their integration into sensing applications by highlighting their various structural advantages in producing highly sensitive tools that rival bench-top instruments. The developments in material design open a new door for decentralized medicine and public protection that allows effective onsite and point-of-care diagnostics.
