The effect of the ATM inhibitor AZD0156 on the radiosensitivity of human breast cancer and lung fibroblast cells

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dc.authorscopusid7005388789
dc.authorscopusid58165647600
dc.authorscopusid56441719900
dc.authorscopusid58166898000
dc.authorscopusid6603599022
dc.authorscopusid6508205483
dc.contributor.authorYilmaz, U.
dc.contributor.authorKamer, D.
dc.contributor.authorAsik, A.
dc.contributor.authorKara, H.G.
dc.contributor.authorGündüz, C.
dc.contributor.authorKamer, S.
dc.date.accessioned2024-08-25T18:48:08Z
dc.date.available2024-08-25T18:48:08Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractAims: To evaluate the effect of the combination of irradiation and AZD0156 on apoptosis, cell cycle progression, and clonogenic survival in human breast cancer and fibroblast cells. Methods and Material: Estrogen receptor-positive breast cancer cell line MCF-7 and healthy lung fibroblast cell line WI-38 were obtained. Following employing proliferation analysis, cytotoxicity analysis was done to calculate the IC50 values of AZD0156 in MCF-7 and WI-38 cell lines. Following the application of AZD0156 and irradiation, flow cytometry analysis was performed for evaluating cell cycle distribution and the extent of apoptosis. Plating efficiency and surviving fraction were calculated for the clonogenic assay. Statistical Analysis Used: SPSS Statistics for Windows, Version 17.0. (SPSS Inc. Chicago) and GraphPad Prism Version 6.0 for Windows (GraphPad Software, San Diego, California USA) softwares were used to analyze data. Results: AZD0156 and irradiation dose of 2-10 Gy had no effect on apoptosis on MCF-7 cells. The combination treatment of AZD0156 and 2 Gy, 4 Gy, 6 Gy, 8 Gy, and 10 Gy irradiation induced G0/G1 phase arrest by 1.79, 1.79, 1.50, 1.25, and 1.52-fold compared to the control group, respectively on MCF-7 cell lines. Combination treatment of AZD0156 and each different irradiation dose affected clonogenic survival owing to increased radiosensitivity (p: 0.02). AZD0156 and irradiation dose of 2 Gy, 4 Gy, 6 Gy, 8 Gy, and 10 Gy decreased the cell viability rate of WI-38 cells by 1.05, 1.18, 1.22, 1.04, and 1.05-fold compared to the control group, respectively. No efficacy was detected on cell cycle analysis, and clonogenic survival was not significantly decreased in WI-38 cells. Conclusion: The combination use of irradiation and AZD0156 has improved efficacy of tumor cell-specific cell cycle arrest and decreasing clonogenic survival.en_US
dc.identifier.doi10.4103/jcrt.JCRT_1540_20
dc.identifier.endpage207en_US
dc.identifier.issn1998-4138
dc.identifier.issue2en_US
dc.identifier.pmid37006058en_US
dc.identifier.scopus2-s2.0-85151341849en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage203en_US
dc.identifier.urihttps://doi.org/10.4103/jcrt.JCRT_1540_20
dc.identifier.urihttps://hdl.handle.net/11454/102167
dc.identifier.volume19en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNLM (Medline)en_US
dc.relation.ispartofJournal of cancer research and therapeuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240825_Gen_US
dc.subjectAZD0156en_US
dc.subjectbreast canceren_US
dc.subjectirradiationen_US
dc.subjectradiosensitivityen_US
dc.subjectATM proteinen_US
dc.subjectATM protein, humanen_US
dc.subjectAZD0156en_US
dc.subjectapoptosisen_US
dc.subjectbreast tumoren_US
dc.subjectcell survivalen_US
dc.subjectfemaleen_US
dc.subjectfibroblasten_US
dc.subjecthumanen_US
dc.subjectlungen_US
dc.subjectpathologyen_US
dc.subjectradiation toleranceen_US
dc.subjecttumor cell lineen_US
dc.subjectApoptosisen_US
dc.subjectAtaxia Telangiectasia Mutated Proteinsen_US
dc.subjectBreast Neoplasmsen_US
dc.subjectCell Line, Tumoren_US
dc.subjectCell Survivalen_US
dc.subjectFemaleen_US
dc.subjectFibroblastsen_US
dc.subjectHumansen_US
dc.subjectLungen_US
dc.subjectRadiation Toleranceen_US
dc.titleThe effect of the ATM inhibitor AZD0156 on the radiosensitivity of human breast cancer and lung fibroblast cellsen_US
dc.typeArticleen_US

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